Data/hora: 12/06/2024 01:42:11

Biblioteca(s): 	Embrapa Mandioca e Fruticultura.	
Data corrente: 	31/01/2000	
Data da última atualização: 	31/01/2000	
Autoria: 	HALE, S. A.; YOUNG, R. E.; ADELBERG, J. W.; KEESE, R. J.; CAMPER, N. D.	
Afiliação: 	Clemson University Agricultural e Biological Engineering Department Clemson, Clemson University Horticulture Dept. Clemson, Clemson University Plant Pathology e Physiology Clemson, USA.	
Título: 	Bioreactor development for continual-flow, liquid plant tissue culture.	
Ano de publicação: 	1992	
Fonte/Imprenta: 	Acta Horticulturae, n.319, p.107-112, 1992.	
Idioma: 	Inglês	
Conteúdo: 	A continual-flow liquid nutrient plant tissue culture bioreactor was constructed in the form of a Nalgene "Bio-Safe" polycarbonate box that supported 10 Sigma membrane rafts. Polypropylene jars serving as supply and waste reservoirs and flexible silicon tubing connections with the growth chamber permitted autoclavig the bioreactor system as an integral unit. Microbial membrane filters enabled periodic nutrient and gas exchanges without jeorpardizing asepsis of the closed bioreactor system. A septum port permitted sampling of the nutrient medium for composition analyses. Doubling or greater of fresh weight gains have been observed with Rex begonia cv. Peacock, Nicotiana tabacum L. cv. Burley 21 and Citrullus lanatus cv. 'Charlee'. Vitrification is prominent with Citrullus lanatus under current protocols in the bioreactor.	
Palavras-Chave: 	Techniques; Tecnicas de cultura de tecidos.	
Thesagro: 	Automação; Micropropagação.	
Thesaurus Nal: 	automation; micropropagation.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Mandioca e Fruticultura (CNPMF)	
Exemplares: 	CNPMF - (17637-1) - ADD - SP - -- - S11720 -  - S11720 - DIS