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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
26/01/1996 |
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Data da última atualização: |
26/01/1996 |
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Autoria: |
KARASEV, A. V.; BOYKO, V. P.; GOWDA, S.; HILF, M. E.; KOONIN, E. V.; NIBLETT, C. L.; CLINE, K.; GUMPF, D. J.; LEE, R. F.; GARNSEY, S. M.; LEWANDOWSKI, D. J; DAWSON, W. O. |
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Afiliação: |
Citrus Reseaech an Education Center, University of Florida, Lake Alfred, Florida 33850-2299; USDA-ARS, Horticultural Research Laboratory, 2120 Camden Road, Orlando, Florida 32803. |
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Título: |
Complete sequence of the citrus tristeza virus RNA genome. |
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Ano de publicação: |
1995 |
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Fonte/Imprenta: |
Virology, v. 208, p.511-520, 1995. |
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Idioma: |
Inglês |
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Conteúdo: |
The sequence of the entire genome of citrus tristeza virus (CTV), Florida isolate T36, was completed. The 19,296-nt CTV genome encodes 12 open reading frames (ORFs) potentially coding for at least 17 protein products. The 5'-proximal ORF 1a starts at nucleotid 108 and encodes a large polyprotein with calculated MW of 349 kDa containing domains characteristic of (from 5' to 3') two papain-like proteases (P-PRO), a methyltransferase (MT), and a helicase (HEL). Alignment of the putative P-PRO sequences of CTV with the related proteases of beet yellws closrerovirus (BYV) and potyviruses allowed the prediction of catalytic cysteine and and histidine residues as well as two cleavage sites, namely Val-Gly/Gly for the 5' proximal P-PRO domain and Met-Gly/Gly for the 5' distal P-PRO domain. The autoproteolytic cleavage of the polyprotein at these sites would release two N-terminal leader proteins of 54 and 55 kDa, respectively, and a 240-kDa C-terminal fragment containing MT and HEL domains. The apparent duplication of the leader domain distingishes CTV from BYV and accounts for most of the size increase in theORF 1a product of CTV. The downstream ORF 1b encodes a 57-kDa putative RNA-dependent RNA polymerase (RdRp)m which is probably expressed via a +1 ribsomal frameshift. Sequence analysis of the frameshif region suggests that this +1 frameshift probably occurs at a rare arginine codon CGG and that elements of the RNA-dependent RNA secondary structere are unilikely to be involved in this process. MenosThe sequence of the entire genome of citrus tristeza virus (CTV), Florida isolate T36, was completed. The 19,296-nt CTV genome encodes 12 open reading frames (ORFs) potentially coding for at least 17 protein products. The 5'-proximal ORF 1a starts at nucleotid 108 and encodes a large polyprotein with calculated MW of 349 kDa containing domains characteristic of (from 5' to 3') two papain-like proteases (P-PRO), a methyltransferase (MT), and a helicase (HEL). Alignment of the putative P-PRO sequences of CTV with the related proteases of beet yellws closrerovirus (BYV) and potyviruses allowed the prediction of catalytic cysteine and and histidine residues as well as two cleavage sites, namely Val-Gly/Gly for the 5' proximal P-PRO domain and Met-Gly/Gly for the 5' distal P-PRO domain. The autoproteolytic cleavage of the polyprotein at these sites would release two N-terminal leader proteins of 54 and 55 kDa, respectively, and a 240-kDa C-terminal fragment containing MT and HEL domains. The apparent duplication of the leader domain distingishes CTV from BYV and accounts for most of the size increase in theORF 1a product of CTV. The downstream ORF 1b encodes a 57-kDa putative RNA-dependent RNA polymerase (RdRp)m which is probably expressed via a +1 ribsomal frameshift. Sequence analysis of the frameshif region suggests that this +1 frameshift probably occurs at a rare arginine codon CGG and that elements of the RNA-dependent RNA secondary structere are unilikely to be involved i... Mostrar Tudo |
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Palavras-Chave: |
Diseases; Doencas; Polimerase; Transmissao; Transmission. |
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Thesagro: |
Proteína; RNA. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02331naa a2200337 a 4500
001 1634211
005 1996-01-26
008 1995 bl uuuu u00u1 u #d
100 1 $aKARASEV, A. V.
245 $aComplete sequence of the citrus tristeza virus RNA genome.
260 $c1995
520 $aThe sequence of the entire genome of citrus tristeza virus (CTV), Florida isolate T36, was completed. The 19,296-nt CTV genome encodes 12 open reading frames (ORFs) potentially coding for at least 17 protein products. The 5'-proximal ORF 1a starts at nucleotid 108 and encodes a large polyprotein with calculated MW of 349 kDa containing domains characteristic of (from 5' to 3') two papain-like proteases (P-PRO), a methyltransferase (MT), and a helicase (HEL). Alignment of the putative P-PRO sequences of CTV with the related proteases of beet yellws closrerovirus (BYV) and potyviruses allowed the prediction of catalytic cysteine and and histidine residues as well as two cleavage sites, namely Val-Gly/Gly for the 5' proximal P-PRO domain and Met-Gly/Gly for the 5' distal P-PRO domain. The autoproteolytic cleavage of the polyprotein at these sites would release two N-terminal leader proteins of 54 and 55 kDa, respectively, and a 240-kDa C-terminal fragment containing MT and HEL domains. The apparent duplication of the leader domain distingishes CTV from BYV and accounts for most of the size increase in theORF 1a product of CTV. The downstream ORF 1b encodes a 57-kDa putative RNA-dependent RNA polymerase (RdRp)m which is probably expressed via a +1 ribsomal frameshift. Sequence analysis of the frameshif region suggests that this +1 frameshift probably occurs at a rare arginine codon CGG and that elements of the RNA-dependent RNA secondary structere are unilikely to be involved in this process.
650 $aProteína
650 $aRNA
653 $aDiseases
653 $aDoencas
653 $aPolimerase
653 $aTransmissao
653 $aTransmission
700 1 $aBOYKO, V. P.
700 1 $aGOWDA, S.
700 1 $aHILF, M. E.
700 1 $aKOONIN, E. V.
700 1 $aNIBLETT, C. L.
700 1 $aCLINE, K.
700 1 $aGUMPF, D. J.
700 1 $aLEE, R. F.
700 1 $aGARNSEY, S. M.
700 1 $aLEWANDOWSKI, D. J
700 1 $aDAWSON, W. O.
773 $tVirology$gv. 208, p.511-520, 1995.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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| 21. |  | TEIXEIRA, J. de C.; COELHO, E. F.; OLIVEIRA, P. M. de; OLIVEIRA, R. C. de; SILVA, T. S. M. da; GOMES FILHO, O. Secamento parcial do sistema radicular diferenciado na fase produtiva da mangueira nas condições do Norte de Minas. In: CONGRESSO BRASILEIRO DE FRUTICULTURA, 22., 2012, Bento Gonçalves. Anais... Bento Gonçalves: SBF, 2012. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| Registros recuperados : 21 | |
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