Data/hora: 13/06/2024 08:08:24

Biblioteca(s): | Embrapa Mandioca e Fruticultura.
Data corrente: | 01/10/1993
Data da última atualização: | 01/10/1993
Autoria: | LEVY, L.; GUMPF, D. J.
Afiliação: | University of California, Department of Plant Pathology, Riverside, CA 92521, USA.
Título: | Studies on the psorosis disease of citrus and preliminary characterization of a flexuous virus associated with the disease.
Ano de publicação: | 1991
Fonte/Imprenta: | Riverside, California: International Organization of Citrus Virologists, 1991.
Páginas: | p.319-336
Idioma: | Inglês
Notas: | Conference of the International Organization of Citrus Virologists, 11., 1991, Riverside, California, Proceedings...
Conteúdo: | Citrus seedlings were graft-inoculated with psorosis (isolate 90-1-1; Concordia, Argentina). When symptomatic young leaves were ground, homogenized and centrifuged at low speed, infectivity (local lesions on Chenopodium quinoa) remained in the supernatant. Polyethylene glycol precipitation and high-speed centrifugation yielded infective pellets. Infectivity was abolished by RNase treatment, even in 0.3 M NaCl, indicating that ssRNA(s) is essential for biological activity. Protein(s) is also required for infectivity, since it was sensitive to phenol extration and proteinase K treatment. When high speed pellets were subjected to sucrose density gradient centrifugation, none of the single fractions accounted for loaded infectivity, but some of the two-fold combinations were much more highly infectious than expected from the contribution of single fractions. Therefore two components activate each other for infectivity. Protein analysis of slow- and fast-sedimenting components suggested that a protein of about 50 Kd MW is associated with infectivity. We interprete these and other results a indicating that a bipartite virus is associated with psorosis disease. The virus has ssRNA as its genetic material and a 50 Kd polypeptide as one of its structural protein(s).
Thesagro: | Chenopodium Quinoa; Laranja; Laranja Doce; Vírus.
Categoria do assunto: | --
Registro original: | Embrapa Mandioca e Fruticultura (CNPMF)
Exemplares: | CNPMF - (13319-1) - ADC - PL - -- - 634.3040601 - C748p - 1992000183 - DIS