Data/hora: 12/06/2024 19:27:32

Biblioteca(s): | Embrapa Mandioca e Fruticultura.
Data corrente: | 21/09/1993
Data da última atualização: | 21/09/1993
Autoria: | SEMANCIK, J. S.; GUMPF, D. J.; BASH, J. A.
Afiliação: | Department of Plant Patholgy, University of California, Riverside, CA 92521, USA.
Título: | Interactions among the group II citrus viroids: A potential for protection from the cachexia disease?
Ano de publicação: | 1991
Fonte/Imprenta: | Riverside, California: International Organization of Citrus Virologists, 1991.
Páginas: | p.189-195
Idioma: | Inglês
Notas: | Conference of the International Organization of Citrus Virologists, 11., 1991, Riverside, California, Proceedings...
Conteúdo: | Viroids classified in Group II of the Citrus Viroid Catalogue contain agents of both the exocortis and cachexia diseases as determined by reactions on the indexing hosts Etrog citron and Parson's Special mandarin. In spite of these distinct biological expressions, CV-IIa and -IIb display a high degree of nucleotide sequence homolgy. A third nember of the CV-II Group, designated as CV-IIc, has been defined on the basis of relative electrophoretic migration. Because of a 2-4 nucleotide differential within a size range of 296-302 nucleotides, the three Group II viroids can by resolved by sequential polyacrylamide gel electrophoresis (sPAGE). All of the CV-II viroids react positively in homologous and heterologous hybridization tests with cDNA probes. To study interactions among the Group ii viroids, buds from established citron sources containing single viroids, along with a healthy bud were grafted to a cammon seedling citron and forced as four equivalent growing points. The viroid content of successive growth was monitored at 4-6 week intervals. All tissues remained symptomless throughout the experiment. In tissues derived from the healthy bud, the three viroids rapidly became established in equal titers as a mixed infection. By contrast, tissues from the CV-IIc source bud contained either CV-II a or CV-IIb as a predominant second viroid component but not both CV-IIa plus CV-IIb. This demonstration of a possible interference or mutual antagonism between CV-IIa and CV-IIb was most pronounced in tissues derived ...
Palavras-Chave: | Citrus viroids; Crossprotection; Etrog citron; Exocortis; Interference; Sequential PAGE.
Thesaurus Nal: | cachexia.
Categoria do assunto: | --
Registro original: | Embrapa Mandioca e Fruticultura (CNPMF)
Exemplares: | CNPMF - (13294-1) - ADC - PL - -- - 634.3040601 - C748p - 1992000183 - DIS