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| Registros recuperados : 193 | |
| 41. |  | FERNANDES, F. D.; GUIMARAES JUNIOR, R.; VIEIRA, E. A.; FIALHO, J. de F.; MALAQUIAS, J. V. Produtividade e valor nutricional da parte aérea e de raízes tuberosas de oito genótipos de mandioca de indústria. Revista Brasileira de Saúde e Produção Animal, Salvador, v.17, n.1, p.1-12 jan./mar., 2016.| Biblioteca(s): Embrapa Cerrados. |
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| 47. | | RODRIGUES, J. A. S.; MENEZES, C. B. de; GUIMARAES JUNIOR, R.; TABOSA, J. N. Utilização do sorgo na nutrição animal. In: PEREIRA FILHO, I. A.; RODRIGUES, J. A. S. (Ed.). Sorgo: o produtor pergunta, a Embrapa responde. Brasília, DF: Embrapa, 2015. cap. 14, p. 229-246. (Coleção 500 perguntas, 500 respostas).| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 50. | | PULROLNIK, K.; VILELA, L.; MARCHAO, R. L.; SOUZA, K. W. de; GUIMARAES JUNIOR, R.; CARVALHO, A. M. de. Soil carbon contents in integrated crop-livestock-forest and crop-livestock system in the Cerrado region. In: INTERNATIONAL SYMPOSIUM ON GREENHOUSE GASES IN AGRICULTURE, 2., 2016, Campo Grande, MS. Proceedings... Brasília, DF: Embrapa, 2016. p. 492-495.| Biblioteca(s): Embrapa Cerrados. |
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| 56. | | PEREIRA, L. G. R.; MORAES, S. A. de; GUIMARÃES JÚNIOR, R.; ARAUJO, G. G. L. de; VOLTOLINI, T. V. Uso de co-produtos da agroenergia na alimentação animal. In: MUNIZ, E. N.; GOMIDE, C. A. de M.; RANGEL, J. H. de A.; ALMEIDA, S. A.; SÁ, C. O. de; SÁ, J. L. de. (Ed.). Alternativas alimentares para ruminantes II. Aracaju: Embrapa Tabuleiros Costeiros, 2008. cap. 5, p.139-171.| Biblioteca(s): Embrapa Semiárido. |
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| 58. | | OLIVEIRA, E. M. de; GUIMARÃES JÚNIOR, R.; RAMOS, A. K. B.; FERNANDES, F. D.; MARTHA JÚNIOR, G. B.; VILELA, L. Concentração mineral de genótipos de Panicum maximum cultivados em solos de Cerrado. In: SIMPÓSIO NACIONAL CERRADO, 9.; SIMPÓSIO INTERNACIONAL SAVANAS TROPICAIS, 2., 2008, Brasília, DF. Desafios e estratégias para o equilíbrio entre sociedade, agronegócio e recursos naturais: anais... Planaltina, DF: Embrapa Cerrados, 2008. 1 CD-ROM.| Biblioteca(s): Embrapa Cerrados. |
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| 59. | | CARVALHO, A. M. de; SOUZA, L. L. P. de; GUIMARAES JUNIOR, R.; ALVES, P. C. A. C.; VIVALDI, L. J. Cover plants with potential use for crop-livestock integrated systems in the Cerrado region. Pesquisa agropecuária Brasileira, Brasília, DF, v. 46, n. 10, p. 1200-1205, out. 2011 Título em inglês: Plantas de cobertura com potencial de uso para a integração lavoura?pecuária na região do Cerrado.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 60. | | CARVALHO, A. M. de; SOUZA, L. L. P. de; GUIMARAES JUNIOR, R.; ALVES, P. C. A. C.; VIVALDI, L. J. Cover plants with potential use for crop-livestock integrated systems in the Cerrado region. Pesquisa agropecuária Brasileira, Brasília, v. 46, n. 10, p. 1200-1205, out. 2011| Biblioteca(s): Embrapa Cerrados. |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
29/03/2019 |
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Data da última atualização: |
21/06/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 3 |
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Autoria: |
CAMARGO, L. S. de A.; MUNK, M.; SALES, J. N.; WOHLRES-VIANA, S.; QUINTAO, C. C. R.; VIANA, J. H. M. |
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Afiliação: |
LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; MICHELE MUNK, UFJF; JOSE NELIO SALES, UFLA; SABINE WOHLRES-VIANA, UFJF; CAROLINA CAPOBIANGO ROMANO QUINTAO, CNPGL; JOAO HENRIQUE MOREIRA VIANA, Cenargen. |
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Título: |
Differential gene expression between in vivo and in vitro maturation: a comparative study with bovine oocytes derived from the same donor pool. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
JBRA Assisted Reproduction, v. 23, n. 1, p. 7-14, 2019. |
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DOI: |
10.5935/1518-0557.20180084 |
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Idioma: |
Inglês |
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Notas: |
Na publicação: Luiz Sergio Almeida Camargo. |
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Conteúdo: |
Objective: In vitro maturation has been shown to influence gene expression in oocytes, but a common shortcoming in reports on the matter has been the use of different donors in each experimental group thus disregarding donor effects. This study aimed to investigate the abundance of mRNA in oocytes matured in vivo and in vitro obtained from the same group of donors. Methods: A bovine model was used to assess the relative abundance of specific transcripts in in vitro-matured (IN VITRO-OPU) and in vivo-matured (IN VIVO-OPU) oocytes collected from the same donors by transvaginal ovum pick-up (OPU). Transcript abundance in oocytes from the IN VIVO-OPU group and oocytes matured in vitro but retrieved from different cows slaughtered at a commercial abattoir (IN VITRO-Abattoir group) was also compared. Total RNA was extracted from denuded oocytes and cDNA was produced via reverse transcription using an oligo(dT) primer for relative quantification of eight target transcripts by real-time PCR. Results: Oocytes in the IN VITRO-OPU group had lower (p<0.05) abundance of peroxiredoxin 1 (Prdx1), heat shock protein 70.1 (Hsp70.1), growth and differentiation factor 9 (Gdf9), and maternal antigen that embryo requires (Mater) transcripts than the oocytes in the IN VIVO-OPU group, all obtained from the same pool of donor cows. Similar results were seen in the comparisons involving the IN VIVO-OPU and IN VITRO-Abattoir groups (p<0.05). Conclusion: In vitro maturation affected the abundance of polyadenylated transcripts in the oocyte cytoplasm when compared to in vivo maturation induced by exogenous hormones in oocytes collected from the same donor pool. MenosObjective: In vitro maturation has been shown to influence gene expression in oocytes, but a common shortcoming in reports on the matter has been the use of different donors in each experimental group thus disregarding donor effects. This study aimed to investigate the abundance of mRNA in oocytes matured in vivo and in vitro obtained from the same group of donors. Methods: A bovine model was used to assess the relative abundance of specific transcripts in in vitro-matured (IN VITRO-OPU) and in vivo-matured (IN VIVO-OPU) oocytes collected from the same donors by transvaginal ovum pick-up (OPU). Transcript abundance in oocytes from the IN VIVO-OPU group and oocytes matured in vitro but retrieved from different cows slaughtered at a commercial abattoir (IN VITRO-Abattoir group) was also compared. Total RNA was extracted from denuded oocytes and cDNA was produced via reverse transcription using an oligo(dT) primer for relative quantification of eight target transcripts by real-time PCR. Results: Oocytes in the IN VITRO-OPU group had lower (p<0.05) abundance of peroxiredoxin 1 (Prdx1), heat shock protein 70.1 (Hsp70.1), growth and differentiation factor 9 (Gdf9), and maternal antigen that embryo requires (Mater) transcripts than the oocytes in the IN VIVO-OPU group, all obtained from the same pool of donor cows. Similar results were seen in the comparisons involving the IN VIVO-OPU and IN VITRO-Abattoir groups (p<0.05). Conclusion: In vitro maturation affected the abundance of p... Mostrar Tudo |
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Palavras-Chave: |
In vivo maturation; MRNA; Ovum pick-up. |
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Thesaurus NAL: |
Gene expression. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/195183/1/jbra-23-01-0007.pdf
|
|
Marc: |
LEADER 02498naa a2200253 a 4500
001 2107759
005 2022-06-21
008 2019 bl uuuu u00u1 u #d
024 7 $a10.5935/1518-0557.20180084$2DOI
100 1 $aCAMARGO, L. S. de A.
245 $aDifferential gene expression between in vivo and in vitro maturation$ba comparative study with bovine oocytes derived from the same donor pool.$h[electronic resource]
260 $c2019
500 $aNa publicação: Luiz Sergio Almeida Camargo.
520 $aObjective: In vitro maturation has been shown to influence gene expression in oocytes, but a common shortcoming in reports on the matter has been the use of different donors in each experimental group thus disregarding donor effects. This study aimed to investigate the abundance of mRNA in oocytes matured in vivo and in vitro obtained from the same group of donors. Methods: A bovine model was used to assess the relative abundance of specific transcripts in in vitro-matured (IN VITRO-OPU) and in vivo-matured (IN VIVO-OPU) oocytes collected from the same donors by transvaginal ovum pick-up (OPU). Transcript abundance in oocytes from the IN VIVO-OPU group and oocytes matured in vitro but retrieved from different cows slaughtered at a commercial abattoir (IN VITRO-Abattoir group) was also compared. Total RNA was extracted from denuded oocytes and cDNA was produced via reverse transcription using an oligo(dT) primer for relative quantification of eight target transcripts by real-time PCR. Results: Oocytes in the IN VITRO-OPU group had lower (p<0.05) abundance of peroxiredoxin 1 (Prdx1), heat shock protein 70.1 (Hsp70.1), growth and differentiation factor 9 (Gdf9), and maternal antigen that embryo requires (Mater) transcripts than the oocytes in the IN VIVO-OPU group, all obtained from the same pool of donor cows. Similar results were seen in the comparisons involving the IN VIVO-OPU and IN VITRO-Abattoir groups (p<0.05). Conclusion: In vitro maturation affected the abundance of polyadenylated transcripts in the oocyte cytoplasm when compared to in vivo maturation induced by exogenous hormones in oocytes collected from the same donor pool.
650 $aGene expression
653 $aIn vivo maturation
653 $aMRNA
653 $aOvum pick-up
700 1 $aMUNK, M.
700 1 $aSALES, J. N.
700 1 $aWOHLRES-VIANA, S.
700 1 $aQUINTAO, C. C. R.
700 1 $aVIANA, J. H. M.
773 $tJBRA Assisted Reproduction$gv. 23, n. 1, p. 7-14, 2019.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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