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Registro Completo |
Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
22/04/2010 |
Data da última atualização: |
05/02/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
FONSECA, I.; SILVA, P. V.; LANGE, C. C.; GUIMARAES, M. F. M.; WELLER, M. M. D. C. A.; SOUSA, K. R. S.; LOPES, P. S.; GUIMARÃES, J. D.; GUIMARÃES, S. E. F. |
Afiliação: |
ISABELA FONSECA, UFV; PRISCILA VENDRAMINI SILVA, UFV; CARLA CHRISTINE LANGE, CNPGL; MARTA FONSECA MARTINS GUIMARAES, CNPGL; MAYARA MORENA DEL CAMBRE AMARAL WELLER, UFV; KATIENE RÉGIA SILVA SOUSA, UFV; PAULO SÁVIO LOPES, UFV; JOSÉ DOMINGOS GUIMARÃES, UFV; SIMONE E. F. GUIMARÃES, UFV. |
Título: |
Expression profile of genes associated with mastitis in dairy cattle. |
Ano de publicação: |
2009 |
Fonte/Imprenta: |
Genetics and Molecular Biology, Ribeirão Preto, v. 32, n. 4, p. 776-781, 2009. |
DOI: |
https://doi.org/10.1590/S1415-47572009005000074 |
Idioma: |
Inglês |
Conteúdo: |
In order to characterize the expression of genes associated with immune response mechanisms to mastitis, we quantified the relative expression of the IL-2, IL-4, IL-6, IL-8, IL-10, IFN-γ and TNF-α genes in milk cells of healthy cows and cows with clinical mastitis. Total RNA was extracted from milk cells of six Black and White Holstein (BW) cows and six Gyr cows, including three animals with and three without mastitis per breed. Gene expression was analyzed by real-time PCR. IL-10 gene expression was higher in the group of BW and Gyr cows with mastitis compared to animals free of infection from both breeds (p < 0.05). It was also higher in BW Holstein animals with clinical mastitis (p < 0.001), but it was not significant when Gyr cows with and without mastitis were compared (0.05 < p < 0.10). Among healthy cows, BW Holstein animals tended to present a higher expression of all genes studied, with a significant difference for the IL-2 and IFN-γ genes (p < 0.001). For animals with mastitis no significant difference in gene expression was observed between the two breeds. These findings suggest that animals with mastitis develop a preferentially cell-mediated immune response. Further studies including larger samples are necessary to better characterize the gene expression profile in cows with mastitis. |
Palavras-Chave: |
Cytokine; Real-time PCR. |
Thesaurus Nal: |
gene expression; immune response. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/23937/1/Lange-2009-GenMolBiol.pdf
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Marc: |
LEADER 02177naa a2200277 a 4500 001 1697368 005 2024-02-05 008 2009 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1590/S1415-47572009005000074$2DOI 100 1 $aFONSECA, I. 245 $aExpression profile of genes associated with mastitis in dairy cattle.$h[electronic resource] 260 $c2009 520 $aIn order to characterize the expression of genes associated with immune response mechanisms to mastitis, we quantified the relative expression of the IL-2, IL-4, IL-6, IL-8, IL-10, IFN-γ and TNF-α genes in milk cells of healthy cows and cows with clinical mastitis. Total RNA was extracted from milk cells of six Black and White Holstein (BW) cows and six Gyr cows, including three animals with and three without mastitis per breed. Gene expression was analyzed by real-time PCR. IL-10 gene expression was higher in the group of BW and Gyr cows with mastitis compared to animals free of infection from both breeds (p < 0.05). It was also higher in BW Holstein animals with clinical mastitis (p < 0.001), but it was not significant when Gyr cows with and without mastitis were compared (0.05 < p < 0.10). Among healthy cows, BW Holstein animals tended to present a higher expression of all genes studied, with a significant difference for the IL-2 and IFN-γ genes (p < 0.001). For animals with mastitis no significant difference in gene expression was observed between the two breeds. These findings suggest that animals with mastitis develop a preferentially cell-mediated immune response. Further studies including larger samples are necessary to better characterize the gene expression profile in cows with mastitis. 650 $agene expression 650 $aimmune response 653 $aCytokine 653 $aReal-time PCR 700 1 $aSILVA, P. V. 700 1 $aLANGE, C. C. 700 1 $aGUIMARAES, M. F. M. 700 1 $aWELLER, M. M. D. C. A. 700 1 $aSOUSA, K. R. S. 700 1 $aLOPES, P. S. 700 1 $aGUIMARÃES, J. D. 700 1 $aGUIMARÃES, S. E. F. 773 $tGenetics and Molecular Biology, Ribeirão Preto$gv. 32, n. 4, p. 776-781, 2009.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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Biblioteca(s): |
Embrapa Semiárido. |
Data corrente: |
23/01/2024 |
Data da última atualização: |
23/01/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
CARVALHO, H. F. de S.; SILVA, T. G. F. da; GALVINCIO, J. D.; ANTONIO, A. C. D.; MENEZES, R. S. C.; SANTOS, C. V. B. dos; MIRANDA, R. de Q.; NOBREGA, R. L. B.; DOMINGUES, T. F.; SILVA, E. A.; MOURA, M. S. B. de. |
Afiliação: |
HERICA FERNANDA DE SOUSA CARVALHO, UFPE; THIERES GEORGE FREIRE DA SILVA, Federal Rural University of Pernambuco, Serra Talhada Academic Unit, Serra Talhada, PE; JOSICLÊDA DOMICIANO GALVÍNCIO, UFPE; ANTÔNIO CELSO DANTAS ANTONINO, UFPE; ROMULO SIMÕES CEZAR MENEZES, UFPE; CLOVES VILAS BOAS DOS SANTOS, UFPE; RODRIGO DE QUEIROGA MIRANDA, University of Manitoba, Manitoba, Canada; RODOLFO LUIZ BEZERRA NÓBREGA, University of Bristol, Bristol, United Kingdom; TOMAS FERREIRA DOMINGUES, University of São Paulo, Ribeirão Preto, SP; EMANUEL ARAÚJO SILVA, UFRPE; MAGNA SOELMA BESERRA DE MOURA, CPATSA. |
Título: |
Use of terrestrial laser scanner for aboveground biomass estimation in a seasonally dry tropical forest. |
Ano de publicação: |
2023 |
Fonte/Imprenta: |
Revista Brasileira de Geografia Física, v. 16 n. 5, p. 2641-2657, 2023. |
DOI: |
https://doi.org/10.26848/rbgf.v16.5.p2741-2657 |
Idioma: |
Português |
Conteúdo: |
Structural parameters of vegetation and quantification of aboveground biomass (AGB) are important in forest monitoring to understand the vital cycle of ecosystems. This activity is even more challenging in seasonally dry tropical forests such as the Caatinga biome. The feasibility of using the terrestrial laser scanner (TLS) to measure structural parameters of plants and estimate aboveground biomass was investigated as an alternative to traditional methods. The study was conducted in an area of caatinga vegetation in the municipality of Petrolina, PE, Brazil, where three experimental subareas were selected to obtain measurements, cutting, and weighing of plants with a soil surface level diameter greater than 2.5 cm, totaling 97 plants. Scans were performed with the light detection and ranging (LiDAR) technology using TLS to obtain a three-dimensional point cloud. The correlation coefficient (r2) for plant height measured in the field and obtained by TLS was 0.80. The measures of trunk diameter at soil surfacelevel and at heights of 40 and 130 cm showed r2of 0.79, 0.78, and 0.76, respectively. New exponential allometric equations were generated using input variables obtained in the field and point cloud TLS. These results demonstrate the feasibility and potential of using LiDAR-TLS in dry forests, such as caatinga vegetation. This emphasizes the need to deepen approaches, tools, and techniques using this technology throughout the year to detect variations due to senescence and, therefore, the carbon cycle MenosStructural parameters of vegetation and quantification of aboveground biomass (AGB) are important in forest monitoring to understand the vital cycle of ecosystems. This activity is even more challenging in seasonally dry tropical forests such as the Caatinga biome. The feasibility of using the terrestrial laser scanner (TLS) to measure structural parameters of plants and estimate aboveground biomass was investigated as an alternative to traditional methods. The study was conducted in an area of caatinga vegetation in the municipality of Petrolina, PE, Brazil, where three experimental subareas were selected to obtain measurements, cutting, and weighing of plants with a soil surface level diameter greater than 2.5 cm, totaling 97 plants. Scans were performed with the light detection and ranging (LiDAR) technology using TLS to obtain a three-dimensional point cloud. The correlation coefficient (r2) for plant height measured in the field and obtained by TLS was 0.80. The measures of trunk diameter at soil surfacelevel and at heights of 40 and 130 cm showed r2of 0.79, 0.78, and 0.76, respectively. New exponential allometric equations were generated using input variables obtained in the field and point cloud TLS. These results demonstrate the feasibility and potential of using LiDAR-TLS in dry forests, such as caatinga vegetation. This emphasizes the need to deepen approaches, tools, and techniques using this technology throughout the year to detect variations due to senescence an... Mostrar Tudo |
Palavras-Chave: |
Florestas tropicais sazonalmente secas; Laser scanner. |
Thesagro: |
Biomassa; Caatinga; Floresta Tropical; Solo; Vegetação. |
Thesaurus NAL: |
Biomass; Tropical forests. |
Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/260982/1/Use-of-terrestrial-laser-scanner-for-aboveground-biomass-estimation-in-a-seasonally-dry-tropical-forest.-2023.pdf
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Marc: |
LEADER 02641naa a2200361 a 4500 001 2161183 005 2024-01-23 008 2023 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.26848/rbgf.v16.5.p2741-2657$2DOI 100 1 $aCARVALHO, H. F. de S. 245 $aUse of terrestrial laser scanner for aboveground biomass estimation in a seasonally dry tropical forest.$h[electronic resource] 260 $c2023 520 $aStructural parameters of vegetation and quantification of aboveground biomass (AGB) are important in forest monitoring to understand the vital cycle of ecosystems. This activity is even more challenging in seasonally dry tropical forests such as the Caatinga biome. The feasibility of using the terrestrial laser scanner (TLS) to measure structural parameters of plants and estimate aboveground biomass was investigated as an alternative to traditional methods. The study was conducted in an area of caatinga vegetation in the municipality of Petrolina, PE, Brazil, where three experimental subareas were selected to obtain measurements, cutting, and weighing of plants with a soil surface level diameter greater than 2.5 cm, totaling 97 plants. Scans were performed with the light detection and ranging (LiDAR) technology using TLS to obtain a three-dimensional point cloud. The correlation coefficient (r2) for plant height measured in the field and obtained by TLS was 0.80. The measures of trunk diameter at soil surfacelevel and at heights of 40 and 130 cm showed r2of 0.79, 0.78, and 0.76, respectively. New exponential allometric equations were generated using input variables obtained in the field and point cloud TLS. These results demonstrate the feasibility and potential of using LiDAR-TLS in dry forests, such as caatinga vegetation. This emphasizes the need to deepen approaches, tools, and techniques using this technology throughout the year to detect variations due to senescence and, therefore, the carbon cycle 650 $aBiomass 650 $aTropical forests 650 $aBiomassa 650 $aCaatinga 650 $aFloresta Tropical 650 $aSolo 650 $aVegetação 653 $aFlorestas tropicais sazonalmente secas 653 $aLaser scanner 700 1 $aSILVA, T. G. F. da 700 1 $aGALVINCIO, J. D. 700 1 $aANTONIO, A. C. D. 700 1 $aMENEZES, R. S. C. 700 1 $aSANTOS, C. V. B. dos 700 1 $aMIRANDA, R. de Q. 700 1 $aNOBREGA, R. L. B. 700 1 $aDOMINGUES, T. F. 700 1 $aSILVA, E. A. 700 1 $aMOURA, M. S. B. de 773 $tRevista Brasileira de Geografia Física$gv. 16 n. 5, p. 2641-2657, 2023.
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