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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
13/02/2017 |
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Data da última atualização: |
30/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
WELLER, M. M.; ALBINO, R. L.; MARCONDES, M. I.; SILVA, W.; DANIELS, K. M.; CAMPOS, M. M.; DUARTE, M. S.; MESCOUTO, M. L.; SILVA, F. F.; GUIMARÃES, S. E. F. |
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Afiliação: |
M. M. WELLER, UFV; R. L. ALBINO, UFV; M. I. MARCONDES, UFV; W. SILVA, UFV; K. M. DANIELS, Virginia Polytechnic Institute and State University, Blacksburg; MARIANA MAGALHAES CAMPOS, CNPGL; M. S. DUARTE, UFV; M. L. MESCOUTO, UFV; F. F. SILVA, UFV; S. E. F. GUIMARÃES, UFV. |
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Título: |
Effects of nutrient intake level on mammary parenchyma growth and gene expression in crossbred (Holstein × Gyr) prepubertal heifers. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Journal of Dairy Science, v. 99, n. 12, p. 9962-9973, 2016. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract This study investigated the effects of increased nutrient intake levels on prepubertal mammary parenchyma development in crossbreed (Holstein × Gyr) dairy heifers. Eighteen heifers age 3 to 4 mo were fed 1 of 3 nutrient intake levels (n=6 per treatment) designed to sustain an average daily gain of 0.0kg/d (maintenance, MA), 0.5kg/d (low gain, LG), or 1.0kg/d (high gain, HG). Serum blood samples collected on d 42 and 84 after a 12-h fast were analyzed for triglycerides, leptin, insulin, and insulin-like growth factor 1 (IGF-1). Liver and mammary parenchyma were biopsied on d 42 and harvested on d 84 for gene expression analysis. Parenchyma samples were also used for biochemical and histological analysis. Mammary parenchyma weight was lower in HG than in MA or LG heifers, but mammary extraparenchymal fat was greater in HG heifers than in other groups. Heifers fed the HG diet had a greater fraction of ether extract in their parenchyma than the others and a smaller fraction of crude protein in their parenchyma than MA heifers. Moreover, the HG and LG heifers had greater body fat mass than MA heifers. Nutrient intake level had no effect on the number of intraparenchymal adipocytes. Heifers fed the HG diet had greater serum IGF-1 than the others, and serum insulin was lower in the MA than the HG or LG heifers. Liver GHR, IGF1, and IGFBP3 mRNA expression was higher, but IGFBP2 mRNA was lower in HG heifers than in others. The parenchyma mRNA expression of lipogenic markers, such as CD36, ACCA, FASN, and ADIPOR1, was upregulated by nutrient intake level. Significant nutrient intake × time interactions for lipogenic genes during the experimental period indicated variable gene expression depending on the time point of prepubertal mammary gland development. Overall, our data suggest that enhancing nutrient intake increased body fat accumulation and lipogenesis in the mammary gland to the detriment of parenchyma growth. Moreover, increased lipogenesis in the parenchyma of HG heifers may indicate that fat accumulation occurred because of adipocyte hypertrophy and not differences in adipogenesis. The implications of these results for milk yield needs to be elucidated. MenosAbstract This study investigated the effects of increased nutrient intake levels on prepubertal mammary parenchyma development in crossbreed (Holstein × Gyr) dairy heifers. Eighteen heifers age 3 to 4 mo were fed 1 of 3 nutrient intake levels (n=6 per treatment) designed to sustain an average daily gain of 0.0kg/d (maintenance, MA), 0.5kg/d (low gain, LG), or 1.0kg/d (high gain, HG). Serum blood samples collected on d 42 and 84 after a 12-h fast were analyzed for triglycerides, leptin, insulin, and insulin-like growth factor 1 (IGF-1). Liver and mammary parenchyma were biopsied on d 42 and harvested on d 84 for gene expression analysis. Parenchyma samples were also used for biochemical and histological analysis. Mammary parenchyma weight was lower in HG than in MA or LG heifers, but mammary extraparenchymal fat was greater in HG heifers than in other groups. Heifers fed the HG diet had a greater fraction of ether extract in their parenchyma than the others and a smaller fraction of crude protein in their parenchyma than MA heifers. Moreover, the HG and LG heifers had greater body fat mass than MA heifers. Nutrient intake level had no effect on the number of intraparenchymal adipocytes. Heifers fed the HG diet had greater serum IGF-1 than the others, and serum insulin was lower in the MA than the HG or LG heifers. Liver GHR, IGF1, and IGFBP3 mRNA expression was higher, but IGFBP2 mRNA was lower in HG heifers than in others. The parenchyma mRNA expression of lipogenic markers,... Mostrar Tudo |
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Palavras-Chave: |
Dairy heifer; Mammary lipogenesis. |
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Thesaurus Nal: |
nutrigenomics. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 03014naa a2200265 a 4500
001 2063812
005 2023-01-30
008 2016 bl uuuu u00u1 u #d
100 1 $aWELLER, M. M.
245 $aEffects of nutrient intake level on mammary parenchyma growth and gene expression in crossbred (Holstein × Gyr) prepubertal heifers.$h[electronic resource]
260 $c2016
520 $aAbstract This study investigated the effects of increased nutrient intake levels on prepubertal mammary parenchyma development in crossbreed (Holstein × Gyr) dairy heifers. Eighteen heifers age 3 to 4 mo were fed 1 of 3 nutrient intake levels (n=6 per treatment) designed to sustain an average daily gain of 0.0kg/d (maintenance, MA), 0.5kg/d (low gain, LG), or 1.0kg/d (high gain, HG). Serum blood samples collected on d 42 and 84 after a 12-h fast were analyzed for triglycerides, leptin, insulin, and insulin-like growth factor 1 (IGF-1). Liver and mammary parenchyma were biopsied on d 42 and harvested on d 84 for gene expression analysis. Parenchyma samples were also used for biochemical and histological analysis. Mammary parenchyma weight was lower in HG than in MA or LG heifers, but mammary extraparenchymal fat was greater in HG heifers than in other groups. Heifers fed the HG diet had a greater fraction of ether extract in their parenchyma than the others and a smaller fraction of crude protein in their parenchyma than MA heifers. Moreover, the HG and LG heifers had greater body fat mass than MA heifers. Nutrient intake level had no effect on the number of intraparenchymal adipocytes. Heifers fed the HG diet had greater serum IGF-1 than the others, and serum insulin was lower in the MA than the HG or LG heifers. Liver GHR, IGF1, and IGFBP3 mRNA expression was higher, but IGFBP2 mRNA was lower in HG heifers than in others. The parenchyma mRNA expression of lipogenic markers, such as CD36, ACCA, FASN, and ADIPOR1, was upregulated by nutrient intake level. Significant nutrient intake × time interactions for lipogenic genes during the experimental period indicated variable gene expression depending on the time point of prepubertal mammary gland development. Overall, our data suggest that enhancing nutrient intake increased body fat accumulation and lipogenesis in the mammary gland to the detriment of parenchyma growth. Moreover, increased lipogenesis in the parenchyma of HG heifers may indicate that fat accumulation occurred because of adipocyte hypertrophy and not differences in adipogenesis. The implications of these results for milk yield needs to be elucidated.
650 $anutrigenomics
653 $aDairy heifer
653 $aMammary lipogenesis
700 1 $aALBINO, R. L.
700 1 $aMARCONDES, M. I.
700 1 $aSILVA, W.
700 1 $aDANIELS, K. M.
700 1 $aCAMPOS, M. M.
700 1 $aDUARTE, M. S.
700 1 $aMESCOUTO, M. L.
700 1 $aSILVA, F. F.
700 1 $aGUIMARÃES, S. E. F.
773 $tJournal of Dairy Science$gv. 99, n. 12, p. 9962-9973, 2016.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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| Registros recuperados : 132 | |
| 61. |  | SILVA, F. F.; ROCHA, G. S.; RESENDE, M. D. V. de; GUIMARÃES, S. E. F.; PETERNELLI, L. A.; DUARTE, D. A. S.; AZEVEDO, C. Seleção genômica ampla para curvas de crescimento. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, v. 65, n. 5, p. 1519-1526, 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Florestas. |
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| 62. | | OTTO, P. I.; GUIMARÃES, S. E. F.; CALUS, M. P. L.; VANDENPLAS, J.; MACHADO, M. A.; PANETTO, J. C. do C.; SILVA, M. V. G. B. Single-step genome-wide association studies (GWAS) and post-GWAS analyses to identify genomic regions and candidate genes for milk yield in Brazilian Girolando cattle. Journal of Dairy Science, v. 103, n. 11, p. 10347-10360, 2020.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 63. | | SILVA, A. A.; SILVA, D. A.; GUIMARÃES, S. E. F.; LOPES. P. S.; SILVA, F. F.; THOMPSON, G.; COSTA, C. N.; CARVALHEIRA, J. Single-step genomic evaluation using an autoregressive multiple lactation model for Portuguese Holstein cattle. In: In: WORLD CONGRESS ON GENETICS APPLIED TO LIVESTOCK PRODUCTION, 11., 2018, Auckland, New Zealand. Proceedings... Massey University, 2018.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 66. | | SANTOS, P. M. dos; NASCIMENTO, A. C. C.; NASCIMENTO, M.; SILVA, F. F. e; AZEVEDO, C. F.; MOTA, R. R.; GUIMARÃES, S. E. F.; LOPES, P. S. Use of regularized quantile regression to predict the genetic merit of pigs for asymmetric carcass traits. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 53, n. 9, p. 1011-1017, Sept. 2018. Título em português: Uso da regressão quantílica regularizada para predição de mérito genético em suínos quanto a características assimétricas de carcaça.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 67. | | PEIXOTO, J. de O.; GUIMARÃES, S. E. F.; LOPES, P. S.; SOARES, M. A. M.; PIRES, A. V.; BARBOSA, M. V. G.; TORRES, R. de A.; SILVA, M. de A. e. Associations of leptin gene polymorphisms with production traits in pigs. Journal of Animal Breeding and Genetics, Hamburg - Belin, v. 123, p. 378-383, 2006.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 68. | | SILVA, F. F.; JEREZ, E. A. Z.; RESENDE, M. D. V. de; VIANA, J. M. S.; AZEVEDO, C. F.; LOPES, P. S.; NASCIMENTO, M.; LIMA, R. O. de; GUIMARÃES, S. E. F. Bayesian model combining linkage and linkage disequilibrium analysis for low density-based genomic selection in animal breeding. Journal of Applied Animal Research, v. 46, n. 1, p. 873-878, 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Florestas. |
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| 69. | | SOLLERO, B. P.; PAIVA, S. R.; FARIA, D. A.; GUIMARÃES, S. E. F.; EGITO, A. A.; ALBUQUERQUE, M. S. M.; CASTRO, S. T. R.; MURATA, L. S.; MARIANTE, A. S. Estrutura genética de cinco raças naturalizadas e comerciais de suínos do Brasil. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 11., 2006, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2006. p. 185.| Tipo: Resumo em Anais de Congresso | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 70. | | NASCIMENTO, C. S.; MACHADO, M. A.; GUIMARÃES, S. E. F.; MARTINS, M. F.; PEIXOTO, J. de O.; FURLONG, J.; PRATA, M. C. de A.; VERNEQUE, R. da S.; TEODORO, R. L.; LOPES, P. S. Expressed sequenced tags profiling of resistant and susceptible Gyr x Holstein cattle infested with the tick Rhipicephalus (Boophilus) microplus. Genetics and Molecular Research, v. 10, n. 4, 2011. 14 p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Gado de Leite; Embrapa Suínos e Aves. |
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| 71. | | VIEIRA, F. O.; FONSECA, I.; PINTO, I. S. B.; SANTOS, A. S. O.; MARTINS, S. A. V.; FOGAÇA, G. N.; LIMA, K. R.; CARDOSO, F. F.; GUIMARÃES, S. E. F.; MARTINS, M. F. Expression analysis of CCL20 in cells from the milk of gyr cows before and 24 hours after artificial infection with Streptococcus agalactiae. In: CONGRESSO BRASILEIRO BRASILEIRO DE GENÉTICA, 59., 2013, Águas de Lindóia. Resumos... Águas de Lindóia: Sociedade Brasileira de Genética, 2013.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite; Embrapa Pecuária Sul. |
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| 72. | | VIEIRA, F. O.; PINTO, I. S. B.; SANTOS, A. S. O.; MARTINS, S. A. V.; FOGAÇA, G. N.; LIMA, K. R.; CARDOSO, F. F.; GUIMARÃES, S. E. F.; MARTINS, M. F. Expression analysis of CCL20 in cells from the milk of gyr cows before and 24 hours after artificial infection with Streptococcus agalactiae. In: Congresso Brasileiro de Genética, 59., 2013, Águas de Lindóia. Resumos...| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 73. | | MARTINS, M. F.; FONSECA, I.; PINTO, I. S. B.; PEREIRA, W. P.; GUIMARÃES, S. E. F.; FERREIRA, M. B. D.; BRITO, M. A. V. P. e; BRANDAO, H. de M. Expression analysis of IL-1B in cells from the milk of gyr cows before and after artificial infection with Streptococcus agalactiae. In: CONGRESSO BRASILEIRO BRASILEIRO DE GENÉTICA, 57., 2011, Águas de Lindóia. Resumos... Brasília, DF: Sociedade Brasileira de Genética, 2011.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 74. | | DOMINGUES, R.; WOHLRES-VIANA, S.; REIS, D. R. de L.; TEIXEIRA, H. C.; FERREIRA, A. P.; GUIMARÃES, S. E. F.; PRATA, M. C. de A.; FURLONG, J.; VERNEQUE, R. da S.; MACHADO, M. A. Expression of immune response genes in peripheral blood of cattle infested with Rhipicephalus microplus. Genetics and Molecular Research, v. 13, n. 2, p. 4013-21, 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 75. | | FONSECA, I.; SILVA, P. V.; LANGE, C. C.; GUIMARAES, M. F. M.; WELLER, M. M. D. C. A.; SOUSA, K. R. S.; LOPES, P. S.; GUIMARÃES, J. D.; GUIMARÃES, S. E. F. Expression profile of genes associated with mastitis in dairy cattle. Genetics and Molecular Biology, Ribeirão Preto, v. 32, n. 4, p. 776-781, 2009.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 76. | | SILVA, F. F. e; ZAMBRANO, M. F. B.; VARONA, L.; GLÓRIA, L. S.; LOPES, P. S.; SILVA, M. V. G. B.; ARBEX, W. A.; LÁZARO, S. F.; RESENDE, M. D. V. de; GUIMARÃES, S. E. F. Genome association study through nonlinear mixed models revealed new candidate genes for pig growth curves. Scientia Agricola, v. 74, n. 1, 2017. 7 P.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Florestas; Embrapa Gado de Leite. |
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| 77. | | DUARTE, D. A. S.; FORTES, M. R. S.; DUARTE, M. de S.; GUIMARÃES, S. E. F.; VERARDO, L. L.; VERONEZE, R.; RIBEIRO, A. M. F.; LOPES, P. S.; RESENDE, M. D. V. de; SILVA, F. F. e. Genome-wide association studies, meta-analyses and derived gene network for meat quality and carcass traits in pigs. Animal Production Science, v. 58, n. 6, p. 1100-1008, May 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Florestas. |
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| 78. | | PAIVA, S. R.; FARIA, D. A.; SILVÉRIO, V. C.; MCMANUS, C.; EGITO, A. A.; DERGAM, J. A.; GUIMARÃES, S. E. F.; CASTRO, S. R.; ALBUQUERQUE, M. S. M.; MARIANTE, A. S. Genetic variability among brazilian sheep using microsatellites. In: THE ROLE OF BIOTECHNOLOGY, 2005, Turin, Italy. [Anais...]. [S.l.: s.n.], 2005. p. 195-196.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 79. | | SILVA, F. F. e; RESENDE, M. D. V. de; ROCHA, G. S.; DUARTE, D. A. S.; LOPES, P. S.; BRUSTOLIN, O. J. B.; THUS, S.; VIANA, J. M. S.; GUIMARÃES, S. E. F. Genomic growth curves of an outbred pig population. Genetics and Molecular Biology, v. 36, n. 4, p. 520-527, 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Florestas. |
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| 80. | | AZEVEDO, A. L. S.; GOMES, A. L.; GASPARINI, K.; CAMPOS, A. L.; DOMINGUES, R.; VIEIRA, T. R.; MENDONÇA, P. R. F.; GUIMARÃES, S. E. F.; PEIXOTO, M. G. C. D.; VERNEQUE, R. S.; REGITANO, L. C. A.; MACHADO, M. A. Identificação de novos alelos microssatélites na raça Gir. In: CONGRESSO BRASILEIRO DE GENÉTICA. 54. 2008, Anais. Salvador. SBG, 2008. p. 228| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| Registros recuperados : 132 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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