Data/hora: 21/06/2024 05:28:53

Biblioteca(s): | Embrapa Agricultura Digital.
Data corrente: | 03/06/2009
Data da última atualização: | 30/11/2010
Autoria: | BERTIOLI, S. C. de M. L.; GUIMARÃES, P. M.; BERTIOLI, D. J.
Afiliação: | SORAYA CRISTINA DE M LEAL BERTIOLI, CENARGEN; PATRICIA MESSEMBERG GUIMARÃES, CENARGEN; DAVID JOHN BERTIOLI, Universidade Católica de Brasília.
Título: | Targeting and genotyping RGAs in a mapping population of the AA genome of wild Arachis.
Ano de publicação: | 2007
Fonte/Imprenta: | Crop Breeding and Applied Biotechnology, Londrina, v. 7, n. 2, p. 179-185, Mar. 2007.
Idioma: | Inglês
Conteúdo: | Peanut is an important legume crop. Although it has high levels of morphological diversity, it lacks genetic variability and sources of disease resistance. The transference of resistance genes from wild species is difficult due to the different ploidy level of the wild and cultivated species. Recently, amphidiploids have been produced that can be used as ‘bridges’ to introgress wild genes. Molecular markers are useful to pyramidize desirable genes and track them through generations of backcrossings. Molecular markers based on Resistance Gene Analogs have improved chances to be present in or linked to resistance gene loci. This study describes the development and genotyping of molecular markers based on resistance gene motifs. Specific primers were designed based on unique sequences of an Arachis RGA dataset. The identity of the amplified polymorphic bands was confirmed by sequencing. These markers were genotyped on a F 2 population that segregates for resistance to biotic stress types.
Palavras-Chave: | Abalho descreve o Primers específicos; Análogos a genes de resistência; Genotipagem de marcadores moleculares; Marcadores moleculares; Seleção assistida por marcadores.
Thesagro: | Agricultura; Amendoim; Biotecnologia; Genética.
Thesaurus Nal: | Arachis.
Categoria do assunto: | G Melhoramento Genético
Registro original: | Embrapa Agricultura Digital (CNPTIA)
Exemplares: | CNPTIA - (13089-1) - ADD - AP - PP -  -  -  - DIS