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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
05/08/2009 |
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Data da última atualização: |
30/05/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FIGUEIREDO, J. E. F.; GOMES, E. A.; GUIMARAES, C. T.; LANA, U. G. de P.; TEIXEIRA, M. A.; LIMA, G. V. C.; BRESSAN, W. |
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Afiliação: |
JOSE EDSON FONTES FIGUEIREDO, CNPMS; ELIANE APARECIDA GOMES, CNPMS; CLAUDIA TEIXEIRA GUIMARAES, CNPMS; UBIRACI GOMES DE PAULA LANA, CNPMS; Maria Aparecida Teixeira, CNPMS; Guilherme Vitor Correa Lima, Fundação Educacional Monsenhor Messias; WELLINGTON BRESSAN, CNPMS. |
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Título: |
Molecular analysis endophytic bacteria from the genus Bacillus isolated from tropical maize (Zea mays L.). |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
Brazilian Journal of Microbiology, São Paulo, v. 40, n. 3, p. 522-534, 2009. |
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DOI: |
10.1590/S1517-83822009000300014 |
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Idioma: |
Inglês |
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Conteúdo: |
Endophytíc bacteria play an important role in agriculture by improving plant performance and adaptation against biotic and abiotic stresses. In the present study molecular methods were used for identifying Bacillus endophytic bacteria isolated from Brazilian sweet corn. SDS-PAGE of whole-cell protein extract of forty-two isolates revealed a high number of scrutinable bands. Twenty-four isolates were identified in nine different groups of duplicated bacteria and eighteen were identified as unique. Some high-accumulated polipeptides with variable length were observed in almost isolates. Partial sequencing of 16S ribosonal gene revealed that all isolates are Bacillus sp. and among thirteen isolates with similar protein profiles, two were different strains. Among the forty-two isolates identified by rDNA sequencing. Bacillus. subitilis and B. pumilus were the most frequenty species (15 and 12 isolates. respectively) followed by B. licheniforms (7 isolates), B. cereus (5 isolates) and B. uniloliquefascelns (3 isolates). According to present results. SDS-P AGE techínique could be used as a fast and cheap first tool for identifying inter-specific variation in maize endophytic bacterial collections while rDNA sequencing could be applied for analyzing intra-specific variation among isolates with similar protein profile as well as for taxonomic studies. |
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Palavras-Chave: |
Bacillus; Endophytíc bacteria; rDNA sequencing; SDS-PAGE; Sweet corn. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/31303/1/Molecular-analysis.pdf
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Marc: |
LEADER 02190naa a2200265 a 4500
001 1488802
005 2018-05-30
008 2009 bl uuuu u00u1 u #d
024 7 $a10.1590/S1517-83822009000300014$2DOI
100 1 $aFIGUEIREDO, J. E. F.
245 $aMolecular analysis endophytic bacteria from the genus Bacillus isolated from tropical maize (Zea mays L.).$h[electronic resource]
260 $c2009
520 $aEndophytíc bacteria play an important role in agriculture by improving plant performance and adaptation against biotic and abiotic stresses. In the present study molecular methods were used for identifying Bacillus endophytic bacteria isolated from Brazilian sweet corn. SDS-PAGE of whole-cell protein extract of forty-two isolates revealed a high number of scrutinable bands. Twenty-four isolates were identified in nine different groups of duplicated bacteria and eighteen were identified as unique. Some high-accumulated polipeptides with variable length were observed in almost isolates. Partial sequencing of 16S ribosonal gene revealed that all isolates are Bacillus sp. and among thirteen isolates with similar protein profiles, two were different strains. Among the forty-two isolates identified by rDNA sequencing. Bacillus. subitilis and B. pumilus were the most frequenty species (15 and 12 isolates. respectively) followed by B. licheniforms (7 isolates), B. cereus (5 isolates) and B. uniloliquefascelns (3 isolates). According to present results. SDS-P AGE techínique could be used as a fast and cheap first tool for identifying inter-specific variation in maize endophytic bacterial collections while rDNA sequencing could be applied for analyzing intra-specific variation among isolates with similar protein profile as well as for taxonomic studies.
653 $aBacillus
653 $aEndophytíc bacteria
653 $arDNA sequencing
653 $aSDS-PAGE
653 $aSweet corn
700 1 $aGOMES, E. A.
700 1 $aGUIMARAES, C. T.
700 1 $aLANA, U. G. de P.
700 1 $aTEIXEIRA, M. A.
700 1 $aLIMA, G. V. C.
700 1 $aBRESSAN, W.
773 $tBrazilian Journal of Microbiology, São Paulo$gv. 40, n. 3, p. 522-534, 2009.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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| 1. |  | VIGNOLO, A. M. dos S.; PEREIRA, A. A. A.; FAGUNDES, L. F.; SILVA, C. A. da; LOVATO, P. E. A produção de arroz orgânico nos assentamentos da reforma agrária na região perimetropolitana de Porto Alegre, RS. Cadernos de Ciência & Tecnologia, Brasília, DF, v. 28, n. 2, p. 447-466, maio./ago. 2011.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 2. | | FARIA, B. M. de; GENRO, T. C. M.; PRATES, E. R.; MINHO, A. P.; NALERIO, E. S.; GARAGORRY, F. C.; FAGUNDES, L. F. S.; ROSSETTO, J. Características estruturais e desempenho de bovinos de corte em pastagem natural com diferentes níveis de intensificação. In: CONGRESSO BRASILEIRO DE ZOOTECNIA, 25., 2015, Fortaleza. Dimensões tecnológicas e sociais da zootecnia: anais. Fortaleza: Sociedade Brasileira de Zootecnia, 2015.| Tipo: Artigo em Anais de Congresso |
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