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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão; Embrapa Soja. |
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Data corrente: |
27/07/2017 |
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Data da última atualização: |
14/08/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GUIMARÃES, C. M.; STONE, L. F.; ZITO, R. K. |
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Afiliação: |
CLEBER MORAIS GUIMARAES, CNPAF; LUIS FERNANDO STONE, CNPAF; ROBERTO KAZUHIKO ZITO, CNPSo. |
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Título: |
Susceptibility of common bean and soybean to water stress evaluated at the sitis phenotyping platform. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Bioscience Journal, Uberlândia, v. 33, n. 4, p. 871-880, July/Aug. 2017. |
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ISSN: |
1981-3163 |
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Idioma: |
Inglês |
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Conteúdo: |
The great challenge of breeding programs focused on tolerance to water stress is the precise, in large scale, and automated phenotyping. Therefore, the objective of this study was to assess whether the controlled conditions of SITIS Automated Phenotyping Platform and the protocol used are suitable for discriminating cultivars of common bean and soybean for tolerance to this stress. Two experiments were carried out in randomized block design, in a split plot scheme, with four replications. The main plots had five water regimes, applied after flowering: daily replacement of 100 (control), 80, 60, 40, and 20% of water evapotranspired in control. The subplots consisted of two common bean cultivars (BRS Pontal and BRS Pérola) in the 1st experiment, and two soybean cultivars (MG/BR 46 Conquista and BR-16) in the 2nd one. In each species, the first cultivar is more tolerant to water stress, and the second one is more susceptible. It is possible to use the SITIS Platform and the proposed protocol to evaluate common bean and soybean cultivars for tolerance to water stress. The common bean cultivars evaluated were more sensitive to this stress than soybeans. The best water status of cultivars BRS Pontal and MG/BR 46 Conquista under water stress confirmed their greater tolerance. |
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Palavras-Chave: |
Fenotipagem; Plataforma SITIS; Temperatura das folhas. |
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Thesagro: |
Deficiência hídrica; Evapotranspiração; Feijão; Glycine max; Phaseolus vulgaris; Soja. |
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Thesaurus Nal: |
Soybeans. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/162125/1/CNPAF-2017-bj.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/162147/1/871.pdf
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Marc: |
LEADER 02106naa a2200277 a 4500
001 2073231
005 2017-08-14
008 2017 bl uuuu u00u1 u #d
022 $a1981-3163
100 1 $aGUIMARÃES, C. M.
245 $aSusceptibility of common bean and soybean to water stress evaluated at the sitis phenotyping platform.$h[electronic resource]
260 $c2017
520 $aThe great challenge of breeding programs focused on tolerance to water stress is the precise, in large scale, and automated phenotyping. Therefore, the objective of this study was to assess whether the controlled conditions of SITIS Automated Phenotyping Platform and the protocol used are suitable for discriminating cultivars of common bean and soybean for tolerance to this stress. Two experiments were carried out in randomized block design, in a split plot scheme, with four replications. The main plots had five water regimes, applied after flowering: daily replacement of 100 (control), 80, 60, 40, and 20% of water evapotranspired in control. The subplots consisted of two common bean cultivars (BRS Pontal and BRS Pérola) in the 1st experiment, and two soybean cultivars (MG/BR 46 Conquista and BR-16) in the 2nd one. In each species, the first cultivar is more tolerant to water stress, and the second one is more susceptible. It is possible to use the SITIS Platform and the proposed protocol to evaluate common bean and soybean cultivars for tolerance to water stress. The common bean cultivars evaluated were more sensitive to this stress than soybeans. The best water status of cultivars BRS Pontal and MG/BR 46 Conquista under water stress confirmed their greater tolerance.
650 $aSoybeans
650 $aDeficiência hídrica
650 $aEvapotranspiração
650 $aFeijão
650 $aGlycine max
650 $aPhaseolus vulgaris
650 $aSoja
653 $aFenotipagem
653 $aPlataforma SITIS
653 $aTemperatura das folhas
700 1 $aSTONE, L. F.
700 1 $aZITO, R. K.
773 $tBioscience Journal, Uberlândia$gv. 33, n. 4, p. 871-880, July/Aug. 2017.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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Registro Completo
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
25/08/2017 |
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Data da última atualização: |
23/09/2019 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
OLIVARES, C. C. S.; SOUZA-FABJAN, J. M. G.; PENEIRAS, A. B. V.; BALARO, M. F. A.; FREITAS, V. J. F.; FONSECA, J. F. da; BRANDÃO, F. Z. |
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Afiliação: |
Universidade Federal Fluminense (UFF); Universidade Estadual do Ceará (UECe) - Fortaleza, CE.; JEFERSON FERREIRA DA FONSECA, CNPC. |
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Título: |
The influence of different methods of frozen-thawed ovine spermatozoa selection on sperm capacitation and viability after incubation. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Animal Reproduction, v. 12, n. 3, p. 554, Jul./Sept. 2015. |
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Idioma: |
Inglês |
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Notas: |
Proceedings of the 29th Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Gramado, RS, Brazil, August 20 to 23, 2015. Abstracts. |
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Conteúdo: |
Abstract: Sperm capacitation is an essential event for fertilization; however, it decreases the sperm lifespan and viability. The aim of this study was to evaluate the effects of four sperm selection techniques on sperm capacitation and viability after incubation. A pool of frozen-thawed sperm from 10 Santa Inês rams was used. The samples were submitted to one of the following sperm selection techniques: sperm washing, Percoll gradient, mini-Percoll gradient, Swim-up and control group. At mini-Percoll technique, was used 400 microliters of 90% and 45% gradients and a centrifugation at 500 xg for 5 minutes. In Percoll, was used 1 mL of each gradient and a centrifugation at 700 xg for 10 minutos. During Swim-up, the sperm was incubated in 1 ml of SPERM-TALP for 45 minutos in humidified atmosphere at 37.5oC. Finaly, at sperm washing the sample suffered centrifugation at 300 xg for 8 minutes, using SPERM-TALP. At the end of each treatment, the selected spermatozoa were incubated at 37oC for 1 h, 2 h, and 3 h. Viability was assessed using acridine orange-propidium iodide combination by computer-assisted sperm analysis. Capacitation status was evaluated using chlortetracycline staining and observed under epifluorescence microscopy. Data were analyzed by ANOVA, followed by Tukey test (P<0.05). After 3 h of incubation, the capacitated sperm was decreased (P<0.05) in all treatments. The capacitated sperm rate was similar (P>0.05) among Percoll (36%), mini-Percoll (34%) and Swim-up (30%), and were lower (P<0.05) than control group (47%) and sperm washing (41%), regardless of the time of incubation. The non-capacitated sperm percentage was higher (P<0.05) at 0 h (12%) and decreased after 3 h (1.5%), in all treatments. Regarding to acrosome reacted cells, there was an interaction (P<0.05) between incubation and sperm selection treatment. The acrosome reacted spermatozoa showed a lower percentage (P<0.05) at 0 h (50%) and 1 h (53%) and higher after 3 h (64%). Percoll and mini-Percoll were higher about acrosome reacted spermatozoa (P<0.05; 60% vs. 61%), whereas control group was the lowest (49%). There was an interaction (P<0.05) between incubation and treatment in sperm viability. Viability assays revealed that 0 h resulted in a higher rate (17.5%; P<0.05) of membrane integrity, after all treatments. Swim-up treatment showed a higher membrane integrity rate (17.4%; P<0.05), regardless of time of incubation. In conclusion, the incubation affects the capacitation status and viability of frozen-thawed ovine sperm. Sperm selection increases the acrosome reacted cells rate and Swim-up allows better viability during incubation. [Influência de diferentes métodos de seleção de espermatozoides ovinos congelados sobre a capacitação e vitalidade espermática após incubação]. Resumo: Apesar de necessária para a fecundação, a capacitação espermática diminui a longevidade e vitalidade dos espermatozoides. Objetivou-se analisar os efeitos de quatro métodos de seleção espermática sobre a capacitação e vitalidade dos espermatozoides após incubação. Foi utilizado pool de sêmen congelado comercial de 10 carneiros da raça Santa Inês. As amostras foram submetidas aos diferentes métodos de seleção: lavagem por centrifugação, gradiente de Percoll, mini-Percoll, Swim-up e grupo controle. Na técnica de mini-Percoll, foi utilizado 400 microlitros dos gradientes de 90% e 45% e a amostra foi submetida a centrifugação por 5 minutos a 5000 xg. Para a técnica de Percoll, foi utilizado 1 mL de cada gradiente e os espermatozoides foram submetidos à força de 700 xg por 10 minutos. Durante o Swim-up, os espermatozoides foram incubados em 1 mL de SPERM-TALP por 45 minutos em atmosfera humidificada a 37,5oC. Já na lavagem por centrifugação, a amostra foi submetida a centrifugação por 8 minutos a 300 x g em SPERM-TALP. Posteriormente, os espermatozoides selecionados foram incubados a 37º C por 1 h, 2 h e 3 h. Foi avaliada a vitalidade por meio de iodedo de propídeo e laranja de acridina pelo sistema de avaliação seminal computadorizada. A capacitação espermática foi analisada pela coloração de hidroclorido de clortetraciclina em microscópio de epifluorescência. O efeito do método sobre os parâmetros foi avaliado pela ANOVA, seguido pelo teste de Tukey (P<0,05). Após 3 h, houve decréscimo da taxa de espermatozoides capacitados (P<0,05) em todos os métodos de seleção. Independente do momento de incubação, a taxa de capacitados foi similar (P>0,05) entre Percoll (36%), mini-Percoll (34%) e Swim-up (30%), as quais foram inferiores (P<0,05) ao grupo controle (47%) e lavagem por centrifugação (41%). A taxa de espermatozoides não capacitados foi superior (P<0,05) no momento 0 h (12%) e diminuiu após 3 h (1,5%), independentemente do método. Houve interação (P<0,05) entre intervalo de incubação e método na taxa de espermatozoides reagidos. O índice de reagidos foi menor (P<0,05) em 0 h (50%) e 1 h (53%) e maior após 3 h (64%). Percoll (60%) e mini-Percoll (61%) apresentaram maiores valores para reagidos (P<0,05) enquanto o controle apresentou o menor (49%). Observou-se interação (P<0,05) entre intervalo de incubação e método na vitalidade espermática. A taxa de íntegros em 0 h foi a maior (17,5%; P<0,05), após os diferentes métodos. O Swim-up apresentou maior taxa de íntegros (17,4%; P<0,05), independentemente do intervalo de incubação. Em conclusão, o intervalo de incubação interfere nos padrões de capacitação e vitalidade de espermatozoides ovinos congelados. A seleção espermática aumenta a taxa de células reagidas e o Swim-up permite maior vitalidade durante a incubação. MenosAbstract: Sperm capacitation is an essential event for fertilization; however, it decreases the sperm lifespan and viability. The aim of this study was to evaluate the effects of four sperm selection techniques on sperm capacitation and viability after incubation. A pool of frozen-thawed sperm from 10 Santa Inês rams was used. The samples were submitted to one of the following sperm selection techniques: sperm washing, Percoll gradient, mini-Percoll gradient, Swim-up and control group. At mini-Percoll technique, was used 400 microliters of 90% and 45% gradients and a centrifugation at 500 xg for 5 minutes. In Percoll, was used 1 mL of each gradient and a centrifugation at 700 xg for 10 minutos. During Swim-up, the sperm was incubated in 1 ml of SPERM-TALP for 45 minutos in humidified atmosphere at 37.5oC. Finaly, at sperm washing the sample suffered centrifugation at 300 xg for 8 minutes, using SPERM-TALP. At the end of each treatment, the selected spermatozoa were incubated at 37oC for 1 h, 2 h, and 3 h. Viability was assessed using acridine orange-propidium iodide combination by computer-assisted sperm analysis. Capacitation status was evaluated using chlortetracycline staining and observed under epifluorescence microscopy. Data were analyzed by ANOVA, followed by Tukey test (P<0.05). After 3 h of incubation, the capacitated sperm was decreased (P<0.05) in all treatments. The capacitated sperm rate was similar (P>0.05) among Percoll (36%), mini-Percoll (34%) and Swim-up (3... Mostrar Tudo |
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Palavras-Chave: |
Embryo Technology; Longevidade espermática; Raça Santa Inês; Seleção espermática; Sperm longevity; Sperm selection. |
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Thesagro: |
Carneiro; Espermatozóide; Ovino; Reprodução animal; Sêmen; Transferência de Embrião. |
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Thesaurus NAL: |
Rams; Reproduction; Sheep. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/163050/1/cnpc-2015-The-influence.pdf
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Marc: |
LEADER 07025nam a2200373 a 4500
001 2074502
005 2019-09-23
008 2015 bl uuuu u00u1 u #d
100 1 $aOLIVARES, C. C. S.
245 $aThe influence of different methods of frozen-thawed ovine spermatozoa selection on sperm capacitation and viability after incubation.$h[electronic resource]
260 $aAnimal Reproduction, v. 12, n. 3, p. 554, Jul./Sept. 2015.$c2015
500 $aProceedings of the 29th Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Gramado, RS, Brazil, August 20 to 23, 2015. Abstracts.
520 $aAbstract: Sperm capacitation is an essential event for fertilization; however, it decreases the sperm lifespan and viability. The aim of this study was to evaluate the effects of four sperm selection techniques on sperm capacitation and viability after incubation. A pool of frozen-thawed sperm from 10 Santa Inês rams was used. The samples were submitted to one of the following sperm selection techniques: sperm washing, Percoll gradient, mini-Percoll gradient, Swim-up and control group. At mini-Percoll technique, was used 400 microliters of 90% and 45% gradients and a centrifugation at 500 xg for 5 minutes. In Percoll, was used 1 mL of each gradient and a centrifugation at 700 xg for 10 minutos. During Swim-up, the sperm was incubated in 1 ml of SPERM-TALP for 45 minutos in humidified atmosphere at 37.5oC. Finaly, at sperm washing the sample suffered centrifugation at 300 xg for 8 minutes, using SPERM-TALP. At the end of each treatment, the selected spermatozoa were incubated at 37oC for 1 h, 2 h, and 3 h. Viability was assessed using acridine orange-propidium iodide combination by computer-assisted sperm analysis. Capacitation status was evaluated using chlortetracycline staining and observed under epifluorescence microscopy. Data were analyzed by ANOVA, followed by Tukey test (P<0.05). After 3 h of incubation, the capacitated sperm was decreased (P<0.05) in all treatments. The capacitated sperm rate was similar (P>0.05) among Percoll (36%), mini-Percoll (34%) and Swim-up (30%), and were lower (P<0.05) than control group (47%) and sperm washing (41%), regardless of the time of incubation. The non-capacitated sperm percentage was higher (P<0.05) at 0 h (12%) and decreased after 3 h (1.5%), in all treatments. Regarding to acrosome reacted cells, there was an interaction (P<0.05) between incubation and sperm selection treatment. The acrosome reacted spermatozoa showed a lower percentage (P<0.05) at 0 h (50%) and 1 h (53%) and higher after 3 h (64%). Percoll and mini-Percoll were higher about acrosome reacted spermatozoa (P<0.05; 60% vs. 61%), whereas control group was the lowest (49%). There was an interaction (P<0.05) between incubation and treatment in sperm viability. Viability assays revealed that 0 h resulted in a higher rate (17.5%; P<0.05) of membrane integrity, after all treatments. Swim-up treatment showed a higher membrane integrity rate (17.4%; P<0.05), regardless of time of incubation. In conclusion, the incubation affects the capacitation status and viability of frozen-thawed ovine sperm. Sperm selection increases the acrosome reacted cells rate and Swim-up allows better viability during incubation. [Influência de diferentes métodos de seleção de espermatozoides ovinos congelados sobre a capacitação e vitalidade espermática após incubação]. Resumo: Apesar de necessária para a fecundação, a capacitação espermática diminui a longevidade e vitalidade dos espermatozoides. Objetivou-se analisar os efeitos de quatro métodos de seleção espermática sobre a capacitação e vitalidade dos espermatozoides após incubação. Foi utilizado pool de sêmen congelado comercial de 10 carneiros da raça Santa Inês. As amostras foram submetidas aos diferentes métodos de seleção: lavagem por centrifugação, gradiente de Percoll, mini-Percoll, Swim-up e grupo controle. Na técnica de mini-Percoll, foi utilizado 400 microlitros dos gradientes de 90% e 45% e a amostra foi submetida a centrifugação por 5 minutos a 5000 xg. Para a técnica de Percoll, foi utilizado 1 mL de cada gradiente e os espermatozoides foram submetidos à força de 700 xg por 10 minutos. Durante o Swim-up, os espermatozoides foram incubados em 1 mL de SPERM-TALP por 45 minutos em atmosfera humidificada a 37,5oC. Já na lavagem por centrifugação, a amostra foi submetida a centrifugação por 8 minutos a 300 x g em SPERM-TALP. Posteriormente, os espermatozoides selecionados foram incubados a 37º C por 1 h, 2 h e 3 h. Foi avaliada a vitalidade por meio de iodedo de propídeo e laranja de acridina pelo sistema de avaliação seminal computadorizada. A capacitação espermática foi analisada pela coloração de hidroclorido de clortetraciclina em microscópio de epifluorescência. O efeito do método sobre os parâmetros foi avaliado pela ANOVA, seguido pelo teste de Tukey (P<0,05). Após 3 h, houve decréscimo da taxa de espermatozoides capacitados (P<0,05) em todos os métodos de seleção. Independente do momento de incubação, a taxa de capacitados foi similar (P>0,05) entre Percoll (36%), mini-Percoll (34%) e Swim-up (30%), as quais foram inferiores (P<0,05) ao grupo controle (47%) e lavagem por centrifugação (41%). A taxa de espermatozoides não capacitados foi superior (P<0,05) no momento 0 h (12%) e diminuiu após 3 h (1,5%), independentemente do método. Houve interação (P<0,05) entre intervalo de incubação e método na taxa de espermatozoides reagidos. O índice de reagidos foi menor (P<0,05) em 0 h (50%) e 1 h (53%) e maior após 3 h (64%). Percoll (60%) e mini-Percoll (61%) apresentaram maiores valores para reagidos (P<0,05) enquanto o controle apresentou o menor (49%). Observou-se interação (P<0,05) entre intervalo de incubação e método na vitalidade espermática. A taxa de íntegros em 0 h foi a maior (17,5%; P<0,05), após os diferentes métodos. O Swim-up apresentou maior taxa de íntegros (17,4%; P<0,05), independentemente do intervalo de incubação. Em conclusão, o intervalo de incubação interfere nos padrões de capacitação e vitalidade de espermatozoides ovinos congelados. A seleção espermática aumenta a taxa de células reagidas e o Swim-up permite maior vitalidade durante a incubação.
650 $aRams
650 $aReproduction
650 $aSheep
650 $aCarneiro
650 $aEspermatozóide
650 $aOvino
650 $aReprodução animal
650 $aSêmen
650 $aTransferência de Embrião
653 $aEmbryo Technology
653 $aLongevidade espermática
653 $aRaça Santa Inês
653 $aSeleção espermática
653 $aSperm longevity
653 $aSperm selection
700 1 $aSOUZA-FABJAN, J. M. G.
700 1 $aPENEIRAS, A. B. V.
700 1 $aBALARO, M. F. A.
700 1 $aFREITAS, V. J. F.
700 1 $aFONSECA, J. F. da
700 1 $aBRANDÃO, F. Z.
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