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Registro Completo |
Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
10/02/2017 |
Data da última atualização: |
30/01/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SALIMENA, A. P. S.; LANGE, C. C.; CAMUSSONE, C.; SIGNORINI, M.; CALVINHO, L. F.; BRITO, M. A. V. P. e; BORGES, C. A. V.; GUIMARAES, A. S.; RIBEIRO, J. B.; MENDONCA, L. C.; PICCOLI, R. H. |
Afiliação: |
Alessandra P. S. Salimena, UFLA; CARLA CHRISTINE LANGE, CNPGL; Cecilia Camussone, INTA; Marcelo Signorini, INTA; Luis F. Calvinho, INTA; MARIA APARECIDA V PAIVA E BRITO, CNPGL; CRISTIANO AMANCIO VIEIRA BORGES, CNPGL; ALESSANDRO DE SA GUIMARAES, CNPGL; JOAO BATISTA RIBEIRO, CNPGL; LETICIA CALDAS MENDONCA, CNPGL; Roberta H. Piccoli, UFLA. |
Título: |
Genotypic and phenotypic detection of capsular polysaccharide and biofilm formation in Staphylococcus aureus isolated from bovine milk collected from Brazilian dairy farms. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
Veterinary Research Communications, v. 40, n. 3, p. 97-106, 2016. |
Idioma: |
Inglês |
Conteúdo: |
Abstract Staphylococcus aureus is a pathogen that frequently causes mastitis in bovine herds worldwide. This pathogen produces several virulence factors, including cell-associated adhesins, toxic and cytolytic exoproteins, and capsular polysaccharides. The aim of the present study was to test for the presence of genes involved in capsular polysaccharide production and biofilm formation in S. aureus isolated from bovine mastitis samples collected from 119 dairy herds located in three different Brazilian regions, as well as to assay the production of capsular polysaccharides and biofilm, in vitro. The detection of the cap, icaAD, and bap genes was performed using PCR. The detection and quantification of capsular polysaccharide production was performed using ELISA assays. The ability of the isolates to form a biofilm was examined using the polystyrene surface of microtiter plates. All 159 S. aureus isolates investigated harboured the cap gene: 80 % carried the cap5 gene and 20 % carried the cap8 gene. Sixty-nine percent of the isolates expressed capsular polysaccharide (CP) in vitro, 58 % expressed CP5 and 11 % expressed CP8. All of the isolates harboured the icaA and icaD genes, and 95.6 % of the isolates carried the bap gene. Of the 159 isolates analysed, 97.5 % were biofilm producers. A significant association between the capsular genotype and phenotype and the amount of biofilm formation was detected: cap5/CP5 isolates tended to form more biofilm and to produce a thinner CP layer than cap8/CP8 isolates. The results indicate a high potential for pathogenicity among S. aureus isolated from bovine milk collected from three different regions in Brazil. MenosAbstract Staphylococcus aureus is a pathogen that frequently causes mastitis in bovine herds worldwide. This pathogen produces several virulence factors, including cell-associated adhesins, toxic and cytolytic exoproteins, and capsular polysaccharides. The aim of the present study was to test for the presence of genes involved in capsular polysaccharide production and biofilm formation in S. aureus isolated from bovine mastitis samples collected from 119 dairy herds located in three different Brazilian regions, as well as to assay the production of capsular polysaccharides and biofilm, in vitro. The detection of the cap, icaAD, and bap genes was performed using PCR. The detection and quantification of capsular polysaccharide production was performed using ELISA assays. The ability of the isolates to form a biofilm was examined using the polystyrene surface of microtiter plates. All 159 S. aureus isolates investigated harboured the cap gene: 80 % carried the cap5 gene and 20 % carried the cap8 gene. Sixty-nine percent of the isolates expressed capsular polysaccharide (CP) in vitro, 58 % expressed CP5 and 11 % expressed CP8. All of the isolates harboured the icaA and icaD genes, and 95.6 % of the isolates carried the bap gene. Of the 159 isolates analysed, 97.5 % were biofilm producers. A significant association between the capsular genotype and phenotype and the amount of biofilm formation was detected: cap5/CP5 isolates tended to form more biofilm and to produce a thinner CP... Mostrar Tudo |
Palavras-Chave: |
Adhesin; Bap; Cap; Capsule; IcaAD. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
Marc: |
LEADER 02594naa a2200301 a 4500 001 2063577 005 2023-01-30 008 2016 bl uuuu u00u1 u #d 100 1 $aSALIMENA, A. P. S. 245 $aGenotypic and phenotypic detection of capsular polysaccharide and biofilm formation in Staphylococcus aureus isolated from bovine milk collected from Brazilian dairy farms.$h[electronic resource] 260 $c2016 520 $aAbstract Staphylococcus aureus is a pathogen that frequently causes mastitis in bovine herds worldwide. This pathogen produces several virulence factors, including cell-associated adhesins, toxic and cytolytic exoproteins, and capsular polysaccharides. The aim of the present study was to test for the presence of genes involved in capsular polysaccharide production and biofilm formation in S. aureus isolated from bovine mastitis samples collected from 119 dairy herds located in three different Brazilian regions, as well as to assay the production of capsular polysaccharides and biofilm, in vitro. The detection of the cap, icaAD, and bap genes was performed using PCR. The detection and quantification of capsular polysaccharide production was performed using ELISA assays. The ability of the isolates to form a biofilm was examined using the polystyrene surface of microtiter plates. All 159 S. aureus isolates investigated harboured the cap gene: 80 % carried the cap5 gene and 20 % carried the cap8 gene. Sixty-nine percent of the isolates expressed capsular polysaccharide (CP) in vitro, 58 % expressed CP5 and 11 % expressed CP8. All of the isolates harboured the icaA and icaD genes, and 95.6 % of the isolates carried the bap gene. Of the 159 isolates analysed, 97.5 % were biofilm producers. A significant association between the capsular genotype and phenotype and the amount of biofilm formation was detected: cap5/CP5 isolates tended to form more biofilm and to produce a thinner CP layer than cap8/CP8 isolates. The results indicate a high potential for pathogenicity among S. aureus isolated from bovine milk collected from three different regions in Brazil. 653 $aAdhesin 653 $aBap 653 $aCap 653 $aCapsule 653 $aIcaAD 700 1 $aLANGE, C. C. 700 1 $aCAMUSSONE, C. 700 1 $aSIGNORINI, M. 700 1 $aCALVINHO, L. F. 700 1 $aBRITO, M. A. V. P. e 700 1 $aBORGES, C. A. V. 700 1 $aGUIMARAES, A. S. 700 1 $aRIBEIRO, J. B. 700 1 $aMENDONCA, L. C. 700 1 $aPICCOLI, R. H. 773 $tVeterinary Research Communications$gv. 40, n. 3, p. 97-106, 2016.
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Embrapa Gado de Leite (CNPGL) |
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Registro Completo
Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
22/01/2014 |
Data da última atualização: |
12/03/2024 |
Tipo da produção científica: |
Artigo em Anais de Congresso |
Autoria: |
PACIULLO, D. S. C.; GOMIDE, C. A. de M.; CASTRO, C. R. T. de; CARVALHO, C. A. B. DE; MAURICIO, R. M.; MORENZ, M. J. F. |
Afiliação: |
DOMINGOS SAVIO CAMPOS PACIULLO, CNPGL; CARLOS AUGUSTO DE MIRANDA GOMIDE, CNPGL; CARLOS RENATO TAVARES DE CASTRO, CNPGL; CARLOS AUGUSTO BRANDÃO DE CARVALHO, UFRRJ; ROGÉRIO M. MAURÍCIO, UFSJ; MIRTON JOSE FROTA MORENZ, CNPGL. |
Título: |
Herbage mass, forage accumulation and nutritive value of Brachiaria decumbens in a silvopastoral system. |
Ano de publicação: |
2013 |
Fonte/Imprenta: |
In: INTERNATIONAL GRASSLAND CONGRESS, 22., 2013, Sydney. Revitalising grasslands to sustain our communities: proceedings. Orange New South Wales: New South Wales Department of Primary Industries, 2013. |
Idioma: |
Inglês |
Palavras-Chave: |
Fibra em detergente neutro; Massa de raízes; Porcentagem de sombreamento; Relação parte aérea/raiz. |
Thesagro: |
Proteína Bruta. |
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/976970/1/Herbage-mass-forage-accumulation-and-nutritive-value.pdf
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Marc: |
LEADER 00889nam a2200217 a 4500 001 1976970 005 2024-03-12 008 2013 bl uuuu u00u1 u #d 100 1 $aPACIULLO, D. S. C. 245 $aHerbage mass, forage accumulation and nutritive value of Brachiaria decumbens in a silvopastoral system.$h[electronic resource] 260 $aIn: INTERNATIONAL GRASSLAND CONGRESS, 22., 2013, Sydney. Revitalising grasslands to sustain our communities: proceedings. Orange New South Wales: New South Wales Department of Primary Industries$c2013 650 $aProteína Bruta 653 $aFibra em detergente neutro 653 $aMassa de raízes 653 $aPorcentagem de sombreamento 653 $aRelação parte aérea/raiz 700 1 $aGOMIDE, C. A. de M. 700 1 $aCASTRO, C. R. T. de 700 1 $aCARVALHO, C. A. B. DE 700 1 $aMAURICIO, R. M. 700 1 $aMORENZ, M. J. F.
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