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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
06/01/2016 |
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Data da última atualização: |
20/03/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GUIMARÃES, A. L. S.; PEREIRA, S. A.; KUSSANO, N. R.; DODE, M. A. N. |
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Afiliação: |
A. L. S. GUIMARÃES, UnB; S. A. PEREIRA, UnB; N. R. KUSSANO, UnB; MARGOT ALVES NUNES DODE, CENARGEN. |
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Título: |
The effect of pre-maturation culture using phosphodiesterase type 3 inhibitor and insulin, transferrin and selenium on nuclear and cytoplasmic maturation of bovine oocytes. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Zygote, p. 1-11, 2015. |
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DOI: |
10.1017/S0967199415000064 |
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Idioma: |
Inglês |
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Conteúdo: |
This study aims to evaluate if a pre-maturation culture (PMC) using cilostamide as a meiotic inhibitor in combination with insulin, transferrin and selenium (ITS) for 8 or 24 h increases in vitro embryo production. To evaluate the effects of PMC on embryo development, cleavage rate, blastocyst rate, embryo size and total cell number were determined. When cilostamide (20 M) was used in PMC for 8 or 24 h, 98% of oocytes were maintained in germinal vesicles. Although the majority of oocytes resumed meiosis after meiotic arrest, the cleavage and blastocyst rates were lower than the control (P < 0.05). When the cilostamide concentration was lowered (10 M) and oocytes were arrested for 8 h, embryo development was improved (P < 0.05) and was similar (P > 0.05) to the control. The deleterious effect of 20 M cilostamide treatment for 24 h on a PMC was confirmed by lower cumulus cell viability, determined by trypan blue staining, in that group compared with the other groups. A lower concentration (10 M) and shorter exposure time (8 h) minimized that effect but did not improve embryo production. More studies should be performed to determine the |
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Palavras-Chave: |
Cilostamide; Cultura pré-maturação; Meiotic arrest; Oócitos bovinos. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/182435/1/effect-of-prematuration-culture-using-phosphodiesterase-type-3-inhibitor-and-insulin-transferrin-and-selenium-on-nuclear-and-cytoplasmic-maturation-of-bovine-oocytes.pdf
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Marc: |
LEADER 01878naa a2200217 a 4500
001 2033071
005 2023-03-20
008 2015 bl uuuu u00u1 u #d
024 7 $a10.1017/S0967199415000064$2DOI
100 1 $aGUIMARÃES, A. L. S.
245 $aThe effect of pre-maturation culture using phosphodiesterase type 3 inhibitor and insulin, transferrin and selenium on nuclear and cytoplasmic maturation of bovine oocytes.$h[electronic resource]
260 $c2015
520 $aThis study aims to evaluate if a pre-maturation culture (PMC) using cilostamide as a meiotic inhibitor in combination with insulin, transferrin and selenium (ITS) for 8 or 24 h increases in vitro embryo production. To evaluate the effects of PMC on embryo development, cleavage rate, blastocyst rate, embryo size and total cell number were determined. When cilostamide (20 M) was used in PMC for 8 or 24 h, 98% of oocytes were maintained in germinal vesicles. Although the majority of oocytes resumed meiosis after meiotic arrest, the cleavage and blastocyst rates were lower than the control (P < 0.05). When the cilostamide concentration was lowered (10 M) and oocytes were arrested for 8 h, embryo development was improved (P < 0.05) and was similar (P > 0.05) to the control. The deleterious effect of 20 M cilostamide treatment for 24 h on a PMC was confirmed by lower cumulus cell viability, determined by trypan blue staining, in that group compared with the other groups. A lower concentration (10 M) and shorter exposure time (8 h) minimized that effect but did not improve embryo production. More studies should be performed to determine the
653 $aCilostamide
653 $aCultura pré-maturação
653 $aMeiotic arrest
653 $aOócitos bovinos
700 1 $aPEREIRA, S. A.
700 1 $aKUSSANO, N. R.
700 1 $aDODE, M. A. N.
773 $tZygote, p. 1-11, 2015.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
22/08/2017 |
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Data da última atualização: |
17/05/2022 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
OLIVEIRA, M. E. F.; NOCITI, R. P.; CAMELA, E. S. C.; PADILHA-NAKAGHI, L. C.; MACIEL, G. S.; RODRIGUEZ, M. G. K.; SANTOS, V. J. C.; FONSECA, J. F. da; VICENTE, W. R. R. |
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Afiliação: |
São Paulo State University (USP) - Jaboticabal, São Paulo, Brazil; USP - Jaboticabal, São Paulo, Brazil; USP - Jaboticabal, São Paulo, Brazil; USP - Jaboticabal, São Paulo, Brazil; USP - Jaboticabal, São Paulo, Brazil; USP - Jaboticabal, São Paulo, Brazil; USP - Jaboticabal, São Paulo, Brazil; JEFERSON FERREIRA DA FONSECA, CNPC; USP - Jaboticabal, São Paulo, Brazil. |
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Título: |
Dynamic of synchronized follicular wave in ewes subjected to different doses of 17b-oestradiol given at the beginning of the progesterone protocol. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Reproduction, Fertility and Development, v. 29, n. 1, p. 118, 2017. |
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Idioma: |
Inglês |
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Notas: |
Proceedings of the 43rd Annual Conference of the International Embryo Technology Society, Austin, Texas, 14-17, January 2017. |
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Conteúdo: |
Abstract: This study was designed to evaluate the effect of different doses of 17beta-oestradiol injection at the beginning of the progesterone protocol on follicular wave dynamic in ewes. In a random day of the oestrous cycle (D0), twenty-four Santa Ines ewes received an intravaginal progesterone device (CIDR®) and a injection of 17beta-oestradiol (E2, Sincrodiol®, Ourofino, Brazil) in different doses (350, 500, and 1000 µg) for G-350E2, G-500E2, and G-1000E2, respectively (n = 8 per group). Ultrasound examinations were performed daily during the CIDR permanence (10 days) using MyLab 30Vet equipment (Esaote, Italy) connected to transrectal linear transducer (frequency of 7.5 MHz). Follicular wave was defined as a follicle or a group of follicles 2 to 3 mm in diameter that grew to 4.5 mm in size before regression or ovulation. The day of wave emergence was based on the day on which the largest follicle of a wave was first detected at 2 or 3?mm (retrospective analysis). Data were analysed by ANOVA followed by a Tukey test (mean ± SEM; P < 0.05) using SAS software. The emergence of a new follicular wave after the beginning of protocols was not detected in 8 out of 24 ewes (33.3%), being 3 from G-350E2, 2 from G-500E2, and 3 from G-1000E2. There was difference (abP = 0.04) for follicular wave emergence day (3.00?±?0.32b, 4.00?±?0.45ab and 5.20?±?0.73a) and averaged day of maximal follicle diameter (8.20 ± 0.58b, 9.50 ± 0.34ab and 9.80 ± 0.20a) for G-350E2, G-500E2 , and G-1000E2, respectively. However, the growing period and the maximum diameter of the largest follicle of this wave did not differ between groups (124.80?±?8.98?h and 5.42 ± 0.25 mm for G-350E2; 132.00 ± 5.37 h and 5.75 ± 0.23 mm for G-500E2; and 110.40 ± 16.28 h and 5.20 ± 0.73 mm for G-1000E2, respectively). In conclusion, the 17beta-oestradiol injection at the beginning of the progesterone protocol was not able to induce the emergence of a new follicular wave in all of females, regardless of dose. However, the synchronized follicular wave occurred earlier in females treated with the lowest dose of the 17beta-oestradiol than those that received the highest dose. MenosAbstract: This study was designed to evaluate the effect of different doses of 17beta-oestradiol injection at the beginning of the progesterone protocol on follicular wave dynamic in ewes. In a random day of the oestrous cycle (D0), twenty-four Santa Ines ewes received an intravaginal progesterone device (CIDR®) and a injection of 17beta-oestradiol (E2, Sincrodiol®, Ourofino, Brazil) in different doses (350, 500, and 1000 µg) for G-350E2, G-500E2, and G-1000E2, respectively (n = 8 per group). Ultrasound examinations were performed daily during the CIDR permanence (10 days) using MyLab 30Vet equipment (Esaote, Italy) connected to transrectal linear transducer (frequency of 7.5 MHz). Follicular wave was defined as a follicle or a group of follicles 2 to 3 mm in diameter that grew to 4.5 mm in size before regression or ovulation. The day of wave emergence was based on the day on which the largest follicle of a wave was first detected at 2 or 3?mm (retrospective analysis). Data were analysed by ANOVA followed by a Tukey test (mean ± SEM; P < 0.05) using SAS software. The emergence of a new follicular wave after the beginning of protocols was not detected in 8 out of 24 ewes (33.3%), being 3 from G-350E2, 2 from G-500E2, and 3 from G-1000E2. There was difference (abP = 0.04) for follicular wave emergence day (3.00?±?0.32b, 4.00?±?0.45ab and 5.20?±?0.73a) and averaged day of maximal follicle diameter (8.20 ± 0.58b, 9.50 ± 0.34ab and 9.80 ± 0.20a) for G-350E2, G-500E2 , and G-1000E... Mostrar Tudo |
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Palavras-Chave: |
Synchronized females. |
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Thesagro: |
Estradiol; Injeção; Ovelha; Ovino; Progesterona; Reprodução animal. |
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Thesaurus NAL: |
Ewes; Injection; Progesterone; Reproduction; Sheep. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 03361nam a2200361 a 4500
001 2074258
005 2022-05-17
008 2017 bl uuuu u00u1 u #d
100 1 $aOLIVEIRA, M. E. F.
245 $aDynamic of synchronized follicular wave in ewes subjected to different doses of 17b-oestradiol given at the beginning of the progesterone protocol.$h[electronic resource]
260 $aReproduction, Fertility and Development, v. 29, n. 1, p. 118$c2017
500 $aProceedings of the 43rd Annual Conference of the International Embryo Technology Society, Austin, Texas, 14-17, January 2017.
520 $aAbstract: This study was designed to evaluate the effect of different doses of 17beta-oestradiol injection at the beginning of the progesterone protocol on follicular wave dynamic in ewes. In a random day of the oestrous cycle (D0), twenty-four Santa Ines ewes received an intravaginal progesterone device (CIDR®) and a injection of 17beta-oestradiol (E2, Sincrodiol®, Ourofino, Brazil) in different doses (350, 500, and 1000 µg) for G-350E2, G-500E2, and G-1000E2, respectively (n = 8 per group). Ultrasound examinations were performed daily during the CIDR permanence (10 days) using MyLab 30Vet equipment (Esaote, Italy) connected to transrectal linear transducer (frequency of 7.5 MHz). Follicular wave was defined as a follicle or a group of follicles 2 to 3 mm in diameter that grew to 4.5 mm in size before regression or ovulation. The day of wave emergence was based on the day on which the largest follicle of a wave was first detected at 2 or 3?mm (retrospective analysis). Data were analysed by ANOVA followed by a Tukey test (mean ± SEM; P < 0.05) using SAS software. The emergence of a new follicular wave after the beginning of protocols was not detected in 8 out of 24 ewes (33.3%), being 3 from G-350E2, 2 from G-500E2, and 3 from G-1000E2. There was difference (abP = 0.04) for follicular wave emergence day (3.00?±?0.32b, 4.00?±?0.45ab and 5.20?±?0.73a) and averaged day of maximal follicle diameter (8.20 ± 0.58b, 9.50 ± 0.34ab and 9.80 ± 0.20a) for G-350E2, G-500E2 , and G-1000E2, respectively. However, the growing period and the maximum diameter of the largest follicle of this wave did not differ between groups (124.80?±?8.98?h and 5.42 ± 0.25 mm for G-350E2; 132.00 ± 5.37 h and 5.75 ± 0.23 mm for G-500E2; and 110.40 ± 16.28 h and 5.20 ± 0.73 mm for G-1000E2, respectively). In conclusion, the 17beta-oestradiol injection at the beginning of the progesterone protocol was not able to induce the emergence of a new follicular wave in all of females, regardless of dose. However, the synchronized follicular wave occurred earlier in females treated with the lowest dose of the 17beta-oestradiol than those that received the highest dose.
650 $aEwes
650 $aInjection
650 $aProgesterone
650 $aReproduction
650 $aSheep
650 $aEstradiol
650 $aInjeção
650 $aOvelha
650 $aOvino
650 $aProgesterona
650 $aReprodução animal
653 $aSynchronized females
700 1 $aNOCITI, R. P.
700 1 $aCAMELA, E. S. C.
700 1 $aPADILHA-NAKAGHI, L. C.
700 1 $aMACIEL, G. S.
700 1 $aRODRIGUEZ, M. G. K.
700 1 $aSANTOS, V. J. C.
700 1 $aFONSECA, J. F. da
700 1 $aVICENTE, W. R. R.
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Embrapa Caprinos e Ovinos (CNPC) |
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