Data/hora: 22/09/2024 21:02:49

Biblioteca(s): 	Embrapa Meio Norte / UEP-Parnaíba.	
Data corrente: 	25/01/1996	
Data da última atualização: 	25/01/1996	
Autoria: 	CARLINI, C. R.; GUIMARAES, J. A.	
Afiliação: 	Universidade Federal Fluminense.	
Título: 	Isolation and characterization of a toxid protein from Canavalia ensiformis (Jack bean) seeds, distinct from concanavalin A.	
Ano de publicação: 	1981	
Fonte/Imprenta: 	Toxicon, v.19, n.5, p.667-675, 1981.	
Idioma: 	Inglês	
Conteúdo: 	A toxic protein present in the crude extract of Canavalia ensiformis seeds induces within 24hr dyspnoea, ataxia, hypothermia, coma, tonic convulsion and death in mice injected i.p. with 100-200mg of protein per kg. The toxing was separated from concanavalin A by affinity adsorption of the latter on sephadex G-100, and further purified by sequentialfractionation by (a) removal of polysaccharide with 30% v/v ethanol; (b) ammonium sulfate precipitation at 0,35-0.55 saturation and (c) DEAE-cellulose chromatography. The purified toxin, within an LD50 of 2-5mg of protein/kg mouse, is unstable. Gel-filtration of the purified toxin on Bio-gel P-200 showed a single protein peak with a molecular weight corresponding to 88,000 daltons. Polyacrylamide gel electrophoresis of this  material indicated the presence of two small contaminants. A central convulsive effect of the toxin can  be proposed since no effects were found on isolated pharmacological preparation. The name CANATOXIN  is suggested for this new toxic protein.	
Palavras-Chave: 	Proteina toxica; Toxic protein.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Meio Norte / UEP-Parnaíba (CPAMN-UEPP)	
Exemplares: 	CPAMN-UEPP - (4817-1) - ADD - SP - -- -  -  - 1996.00027 - DIS