| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Pecuária Sudeste. |
|
Data corrente: |
01/11/2010 |
|
Data da última atualização: |
29/06/2023 |
|
Tipo da produção científica: |
Artigo em Anais de Congresso |
|
Autoria: |
BRITO, L. G.; BARBIERI, F. da S.; OLIVEIRA, M. C. de S.; GUERRERO, F. D. |
|
Afiliação: |
LUCIANA GATTO BRITO, CPAF-RO; FABIO DA SILVA BARBIERI, CPAF-RO; MARCIA CRISTINA DE SENA OLIVEIRA, CPPSE; F. D. GUERRERO, LIVESTOCK INSECTS RESEARCH LABORATORY/KERRVILLE-TX. |
|
Título: |
Pasa assay for diagnosing pyrethroide resistance in the cattle tick populations in Rondonia. |
|
Ano de publicação: |
2010 |
|
Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE PARASITOLOGIA VETERINÁRIA, 16., Campo Grande. Anais... Campo Grqande: CBPV, 2010. |
|
Páginas: |
p. 125 - 126. |
|
Idioma: |
Português |
|
Conteúdo: |
Knockdown (kdr) resistance is caused by a reduction in the sensitivity of the arthropod nervous system to pyrethroids. Kdr resistance in field populations of cattle tick can severely limit pyrethroid usefulness in tick control programs. Early detection and characterization of kdr resistance are critical to the development of resistance management strategies. Cattle tick samples collected in Porto Velho and Presidente Medici, Rondonia were analyzed at Knipling-Bushland U.S. Livestock Insects Research Laboratory, USDA/ARS, Kerrville, TX, to verify the genotypes of these cattle tick populations. First, the populations were assessed using commercial cypermethrin solution by Adult Immersion Test (AIT) conducted at Embrapa Rondonia Animal Health Laboratory. Engorgement females ticks were exposed for 30 minutes in commercial cypermethrin solution prepared according to the manufacturer recommendations. The control group was formed with engorged females cattle ticks belonging to each of the populations tested, which were immersed in distilled water. Three groups of ten ticks were used in each treatment. Ten females by Presidente Medici population and three females by Porto Velho population immersed in commercial cypermethrin solution survived. The survived females were placed in B.O.D to obtain the postures. After the eggs hatch, the larvae were collected and immediately frozen at ultra-low temperature.Genomic DNA was isolated from individual larvae cattle tick and 30 larvae of each population tested by PASA (PCR amplification of specific alleles) assay for the presence of a specific nucleotide substitution in the sodium channel gene sequence that has been associated with kdr resistance in cattle tick. The primers, FG 221 and FG 227 (reaction 1) or FG 222 and FG 227 (reaction 2) produced diagnostic products for genotyping the kdr allele. Reaction products were visualized after 4% agarose gel electrophoresis followed by UV illumination after staining with Syber Green. The kdr allele was detected only in Presidente Medici population, which was considered a pyrethroid heterozygous (SR) population. This result was expected for this cattle tick population as there is report of resistance to pyrethroid pesticides, since the population showed a resistance factor of 18.35 for the pesticide evaluated. MenosKnockdown (kdr) resistance is caused by a reduction in the sensitivity of the arthropod nervous system to pyrethroids. Kdr resistance in field populations of cattle tick can severely limit pyrethroid usefulness in tick control programs. Early detection and characterization of kdr resistance are critical to the development of resistance management strategies. Cattle tick samples collected in Porto Velho and Presidente Medici, Rondonia were analyzed at Knipling-Bushland U.S. Livestock Insects Research Laboratory, USDA/ARS, Kerrville, TX, to verify the genotypes of these cattle tick populations. First, the populations were assessed using commercial cypermethrin solution by Adult Immersion Test (AIT) conducted at Embrapa Rondonia Animal Health Laboratory. Engorgement females ticks were exposed for 30 minutes in commercial cypermethrin solution prepared according to the manufacturer recommendations. The control group was formed with engorged females cattle ticks belonging to each of the populations tested, which were immersed in distilled water. Three groups of ten ticks were used in each treatment. Ten females by Presidente Medici population and three females by Porto Velho population immersed in commercial cypermethrin solution survived. The survived females were placed in B.O.D to obtain the postures. After the eggs hatch, the larvae were collected and immediately frozen at ultra-low temperature.Genomic DNA was isolated from individual larvae cattle tick and 30 larvae of each ... Mostrar Tudo |
|
Palavras-Chave: |
Allele characterization; KDR; Pyrethroid resistance; Rondonia. |
|
Thesaurus Nal: |
Rhipicephalus microplus. |
|
Categoria do assunto: |
G Melhoramento Genético |
|
Marc: |
LEADER 03025nam a2200217 a 4500
001 1865776
005 2023-06-29
008 2010 bl uuuu u00u1 u #d
100 1 $aBRITO, L. G.
245 $aPasa assay for diagnosing pyrethroide resistance in the cattle tick populations in Rondonia.
260 $aIn: CONGRESSO BRASILEIRO DE PARASITOLOGIA VETERINÁRIA, 16., Campo Grande. Anais... Campo Grqande: CBPV$c2010
300 $ap. 125 - 126.
520 $aKnockdown (kdr) resistance is caused by a reduction in the sensitivity of the arthropod nervous system to pyrethroids. Kdr resistance in field populations of cattle tick can severely limit pyrethroid usefulness in tick control programs. Early detection and characterization of kdr resistance are critical to the development of resistance management strategies. Cattle tick samples collected in Porto Velho and Presidente Medici, Rondonia were analyzed at Knipling-Bushland U.S. Livestock Insects Research Laboratory, USDA/ARS, Kerrville, TX, to verify the genotypes of these cattle tick populations. First, the populations were assessed using commercial cypermethrin solution by Adult Immersion Test (AIT) conducted at Embrapa Rondonia Animal Health Laboratory. Engorgement females ticks were exposed for 30 minutes in commercial cypermethrin solution prepared according to the manufacturer recommendations. The control group was formed with engorged females cattle ticks belonging to each of the populations tested, which were immersed in distilled water. Three groups of ten ticks were used in each treatment. Ten females by Presidente Medici population and three females by Porto Velho population immersed in commercial cypermethrin solution survived. The survived females were placed in B.O.D to obtain the postures. After the eggs hatch, the larvae were collected and immediately frozen at ultra-low temperature.Genomic DNA was isolated from individual larvae cattle tick and 30 larvae of each population tested by PASA (PCR amplification of specific alleles) assay for the presence of a specific nucleotide substitution in the sodium channel gene sequence that has been associated with kdr resistance in cattle tick. The primers, FG 221 and FG 227 (reaction 1) or FG 222 and FG 227 (reaction 2) produced diagnostic products for genotyping the kdr allele. Reaction products were visualized after 4% agarose gel electrophoresis followed by UV illumination after staining with Syber Green. The kdr allele was detected only in Presidente Medici population, which was considered a pyrethroid heterozygous (SR) population. This result was expected for this cattle tick population as there is report of resistance to pyrethroid pesticides, since the population showed a resistance factor of 18.35 for the pesticide evaluated.
650 $aRhipicephalus microplus
653 $aAllele characterization
653 $aKDR
653 $aPyrethroid resistance
653 $aRondonia
700 1 $aBARBIERI, F. da S.
700 1 $aOLIVEIRA, M. C. de S.
700 1 $aGUERRERO, F. D.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Pesca e Aquicultura; Embrapa Unidades Centrais. |
|
Data corrente: |
14/11/2016 |
|
Data da última atualização: |
17/11/2016 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
A - 2 |
|
Autoria: |
BORTOLON, L.; ERNANI, P. R.; BORTOLON, E. S. O.; GIANELO, C.; ALMEIDA, R. G. O. de; WELTER, S.; ROGERI, D. A. |
|
Afiliação: |
LEANDRO BORTOLON, CNPASA; PAULO ROBERTO ERNANI, UESC; ELISANDRA SOLANGE OLIVEIRA BORTOLON, CNPASA; CLESIO GIANELLO, UFRGS; RODRIGO GABRIEL OLIVEIRA DE ALMEIDA, UFRGS; SAMUEL WELTER, UFRGS; DOUGLAS ANTONIO ROGERI, Faculdade de Itapiranga. |
|
Título: |
Degree of phosphorus saturation threshold for minimizing P losses by runoff in cropland soil of Southern Brazil. |
|
Ano de publicação: |
2016 |
|
Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, Brasília, DF, v. 51, n. 9, p. 1088-1098, set. 2016. |
|
Idioma: |
Inglês |
|
Conteúdo: |
The objective of this work was to assess the risk of phosphorus losses by runoff through an index based on the degree of P saturation (DPS), in cropland soils of Southern Brazil. Sixty-five highly representative cropland soils from the region were evaluated. Three labile P forms were measured (Mehlich-1, Mehlich-3, and ammonium oxalate), and four P sorption indexes were tested (phosphorus single sorption point and Fe+Al determined with the three extractors). Water-extractable P (WEP) was used as an index of P susceptibility to losses by surface runoff. The DPS was determined from the ratio between labile P and each sorption index. DPS values obtained from the ratio between Mehlich-1 P and the single P sorption point ranged from 1 to 25%, whereas those from Mehlich-1 P and Fe+Al (ammonium oxalate) ranged from 1 to 55%. All DPS types were highly correlated with WEP. From a practical stand point, the DPS obtained with both P and Fe+Al extracted with Mehlich-1 can be used to estimate the risk of P losses by runoff in soils of Southern Brazil. |
|
Palavras-Chave: |
Escorrimento superficial; P em único ponto; Qualidade de água; Single P sorption point. |
|
Thesagro: |
Eutrofização. |
|
Thesaurus NAL: |
Eutrophication; Runoff; Water quality. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/150006/1/Degree-of-phosphorus-saturation.pdf
|
|
Marc: |
LEADER 01951naa a2200289 a 4500
001 2056553
005 2016-11-17
008 2016 bl uuuu u00u1 u #d
100 1 $aBORTOLON, L.
245 $aDegree of phosphorus saturation threshold for minimizing P losses by runoff in cropland soil of Southern Brazil.$h[electronic resource]
260 $c2016
520 $aThe objective of this work was to assess the risk of phosphorus losses by runoff through an index based on the degree of P saturation (DPS), in cropland soils of Southern Brazil. Sixty-five highly representative cropland soils from the region were evaluated. Three labile P forms were measured (Mehlich-1, Mehlich-3, and ammonium oxalate), and four P sorption indexes were tested (phosphorus single sorption point and Fe+Al determined with the three extractors). Water-extractable P (WEP) was used as an index of P susceptibility to losses by surface runoff. The DPS was determined from the ratio between labile P and each sorption index. DPS values obtained from the ratio between Mehlich-1 P and the single P sorption point ranged from 1 to 25%, whereas those from Mehlich-1 P and Fe+Al (ammonium oxalate) ranged from 1 to 55%. All DPS types were highly correlated with WEP. From a practical stand point, the DPS obtained with both P and Fe+Al extracted with Mehlich-1 can be used to estimate the risk of P losses by runoff in soils of Southern Brazil.
650 $aEutrophication
650 $aRunoff
650 $aWater quality
650 $aEutrofização
653 $aEscorrimento superficial
653 $aP em único ponto
653 $aQualidade de água
653 $aSingle P sorption point
700 1 $aERNANI, P. R.
700 1 $aBORTOLON, E. S. O.
700 1 $aGIANELO, C.
700 1 $aALMEIDA, R. G. O. de
700 1 $aWELTER, S.
700 1 $aROGERI, D. A.
773 $tPesquisa Agropecuária Brasileira, Brasília, DF$gv. 51, n. 9, p. 1088-1098, set. 2016.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Pesca e Aquicultura (CNPASA) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
