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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
01/11/2010 |
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Data da última atualização: |
29/06/2023 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
BRITO, L. G.; BARBIERI, F. da S.; OLIVEIRA, M. C. de S.; GUERRERO, F. D. |
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Afiliação: |
LUCIANA GATTO BRITO, CPAF-RO; FABIO DA SILVA BARBIERI, CPAF-RO; MARCIA CRISTINA DE SENA OLIVEIRA, CPPSE; F. D. GUERRERO, LIVESTOCK INSECTS RESEARCH LABORATORY/KERRVILLE-TX. |
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Título: |
Pasa assay for diagnosing pyrethroide resistance in the cattle tick populations in Rondonia. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE PARASITOLOGIA VETERINÁRIA, 16., Campo Grande. Anais... Campo Grqande: CBPV, 2010. |
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Páginas: |
p. 125 - 126. |
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Idioma: |
Português |
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Conteúdo: |
Knockdown (kdr) resistance is caused by a reduction in the sensitivity of the arthropod nervous system to pyrethroids. Kdr resistance in field populations of cattle tick can severely limit pyrethroid usefulness in tick control programs. Early detection and characterization of kdr resistance are critical to the development of resistance management strategies. Cattle tick samples collected in Porto Velho and Presidente Medici, Rondonia were analyzed at Knipling-Bushland U.S. Livestock Insects Research Laboratory, USDA/ARS, Kerrville, TX, to verify the genotypes of these cattle tick populations. First, the populations were assessed using commercial cypermethrin solution by Adult Immersion Test (AIT) conducted at Embrapa Rondonia Animal Health Laboratory. Engorgement females ticks were exposed for 30 minutes in commercial cypermethrin solution prepared according to the manufacturer recommendations. The control group was formed with engorged females cattle ticks belonging to each of the populations tested, which were immersed in distilled water. Three groups of ten ticks were used in each treatment. Ten females by Presidente Medici population and three females by Porto Velho population immersed in commercial cypermethrin solution survived. The survived females were placed in B.O.D to obtain the postures. After the eggs hatch, the larvae were collected and immediately frozen at ultra-low temperature.Genomic DNA was isolated from individual larvae cattle tick and 30 larvae of each population tested by PASA (PCR amplification of specific alleles) assay for the presence of a specific nucleotide substitution in the sodium channel gene sequence that has been associated with kdr resistance in cattle tick. The primers, FG 221 and FG 227 (reaction 1) or FG 222 and FG 227 (reaction 2) produced diagnostic products for genotyping the kdr allele. Reaction products were visualized after 4% agarose gel electrophoresis followed by UV illumination after staining with Syber Green. The kdr allele was detected only in Presidente Medici population, which was considered a pyrethroid heterozygous (SR) population. This result was expected for this cattle tick population as there is report of resistance to pyrethroid pesticides, since the population showed a resistance factor of 18.35 for the pesticide evaluated. MenosKnockdown (kdr) resistance is caused by a reduction in the sensitivity of the arthropod nervous system to pyrethroids. Kdr resistance in field populations of cattle tick can severely limit pyrethroid usefulness in tick control programs. Early detection and characterization of kdr resistance are critical to the development of resistance management strategies. Cattle tick samples collected in Porto Velho and Presidente Medici, Rondonia were analyzed at Knipling-Bushland U.S. Livestock Insects Research Laboratory, USDA/ARS, Kerrville, TX, to verify the genotypes of these cattle tick populations. First, the populations were assessed using commercial cypermethrin solution by Adult Immersion Test (AIT) conducted at Embrapa Rondonia Animal Health Laboratory. Engorgement females ticks were exposed for 30 minutes in commercial cypermethrin solution prepared according to the manufacturer recommendations. The control group was formed with engorged females cattle ticks belonging to each of the populations tested, which were immersed in distilled water. Three groups of ten ticks were used in each treatment. Ten females by Presidente Medici population and three females by Porto Velho population immersed in commercial cypermethrin solution survived. The survived females were placed in B.O.D to obtain the postures. After the eggs hatch, the larvae were collected and immediately frozen at ultra-low temperature.Genomic DNA was isolated from individual larvae cattle tick and 30 larvae of each ... Mostrar Tudo |
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Palavras-Chave: |
Allele characterization; KDR; Pyrethroid resistance; Rondonia. |
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Thesaurus Nal: |
Rhipicephalus microplus. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 03025nam a2200217 a 4500
001 1865776
005 2023-06-29
008 2010 bl uuuu u00u1 u #d
100 1 $aBRITO, L. G.
245 $aPasa assay for diagnosing pyrethroide resistance in the cattle tick populations in Rondonia.
260 $aIn: CONGRESSO BRASILEIRO DE PARASITOLOGIA VETERINÁRIA, 16., Campo Grande. Anais... Campo Grqande: CBPV$c2010
300 $ap. 125 - 126.
520 $aKnockdown (kdr) resistance is caused by a reduction in the sensitivity of the arthropod nervous system to pyrethroids. Kdr resistance in field populations of cattle tick can severely limit pyrethroid usefulness in tick control programs. Early detection and characterization of kdr resistance are critical to the development of resistance management strategies. Cattle tick samples collected in Porto Velho and Presidente Medici, Rondonia were analyzed at Knipling-Bushland U.S. Livestock Insects Research Laboratory, USDA/ARS, Kerrville, TX, to verify the genotypes of these cattle tick populations. First, the populations were assessed using commercial cypermethrin solution by Adult Immersion Test (AIT) conducted at Embrapa Rondonia Animal Health Laboratory. Engorgement females ticks were exposed for 30 minutes in commercial cypermethrin solution prepared according to the manufacturer recommendations. The control group was formed with engorged females cattle ticks belonging to each of the populations tested, which were immersed in distilled water. Three groups of ten ticks were used in each treatment. Ten females by Presidente Medici population and three females by Porto Velho population immersed in commercial cypermethrin solution survived. The survived females were placed in B.O.D to obtain the postures. After the eggs hatch, the larvae were collected and immediately frozen at ultra-low temperature.Genomic DNA was isolated from individual larvae cattle tick and 30 larvae of each population tested by PASA (PCR amplification of specific alleles) assay for the presence of a specific nucleotide substitution in the sodium channel gene sequence that has been associated with kdr resistance in cattle tick. The primers, FG 221 and FG 227 (reaction 1) or FG 222 and FG 227 (reaction 2) produced diagnostic products for genotyping the kdr allele. Reaction products were visualized after 4% agarose gel electrophoresis followed by UV illumination after staining with Syber Green. The kdr allele was detected only in Presidente Medici population, which was considered a pyrethroid heterozygous (SR) population. This result was expected for this cattle tick population as there is report of resistance to pyrethroid pesticides, since the population showed a resistance factor of 18.35 for the pesticide evaluated.
650 $aRhipicephalus microplus
653 $aAllele characterization
653 $aKDR
653 $aPyrethroid resistance
653 $aRondonia
700 1 $aBARBIERI, F. da S.
700 1 $aOLIVEIRA, M. C. de S.
700 1 $aGUERRERO, F. D.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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| Registros recuperados : 19 | |
| 8. |  | GUERRERO, F. D.; LEÓN, A. A. P. de; RODRIGUEZ- VIVAS, R. I.; JONSSON, N.; MILLER, R. J.; ANDREOTTI, R. Acaricide research and development, resistance, and resistance monitoring. In: SONENSHINE, D. E.; ROE, R. M. (Ed.). Biology of ticks. 2nd ed. New York: Oxford, 2014.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Gado de Corte. |
|    |
| 9. | | ANDREOTTI, R.; GUERRERO, F. D.; SOARES, M. A.; BARROS, J. C.; MILLER, R. J.; PÉREZ DE LEÓN, A. Acaricide resistance of Rhipicephalus (Boophilus) microplus in State of Mato Grosso do Sul, Brazil. Revista Brasileira de Parasitologia Veterinária, Jaboticabal, v. 20, n. 2, p. 127-133, Apr.-Jun. 2011.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 3 |
| Biblioteca(s): Embrapa Gado de Corte. |
|  |
| 11. | | CUNHA, R. C.; PÉREZ DE LEÓN, A. A.; LEITE, F. P. L.; GUERRERO, F. D.; PINTO, L. S.; ANDREOTTI, R. Assessment of bovine immunoprotection against Rhipicephalus microplus using Bm86-CG antigen expressed in Pichia pastoris. In: AMERICAN ASSOCIATION OF VETERINARY PARASITOLOGISTS ANNUAL MEETING, 56.; LIVESTOCK INSECT WORKERS CONFERENCE, 55.; INTERNATIONAL SYMPOSIUM ON ECTOPARASITES OF PETS, 11.,2011,ST. LOUIS, MO. Sustainable parasite and vector control: proceedings. St. Louis,MO: American Association of Veterinary Parasitologists, 2011. p.95| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 13. | | SABATINI, G. A.; RIBOLLA, P. E. M.; BARROS, A. T. M. de; GUERRERO, F. D.; SHUMAKER, T. T. S. Knockdown resistance in pyrethroid-resistant horn fly (Diptera: Muscidae) populations in Brazil. Revista Brasileira Parasitologia Veterinária, Jaboticabal, v. 18; n.3, p.311-316, jul/set, 2009.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 3 |
| Biblioteca(s): Embrapa Pantanal. |
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| 14. | | GUERRERO, F. D.; CAMERON, C.; FOIL, L. D.; BARROS, A. T. M. de; CROW, J.; RETZEL, E. Molecular sequence dataset to identify metabolic-based pyrethroid resistance in the horn fly, Haematobia irritans. In: CONGRESSO BRASILEIRO DE PARASITOLOGIA VETERINÁRIA, 17., 2012, São Luis. Parasitologia veterinária, bem estar e produção animal: anais. São Luis: Colégio Brasileiro de Parasitologia Veterinária, 2012. Resumo: PA 149. p. 193| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pantanal. |
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| 15. | | ANDREOTTI, R.; PEREZ DE LEON, A; GUERRERO, F. D.; BENDELE, K. E.; SCOLES, G. A.; DOWD, S. E. Symbiotic bacteria in the cattle tick rhipicephalus microplus. In: CONGRESSO BRASILEIRO DE PARASITOLOGIA VETERINÁRIA, 16., 2010, Campo Grande, MS. [Anais...]. Campo Grande, MS: Colégio Brasileiro de Parasitologia Veterinária, 2010. 1 CD-ROM. 1 p.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 17. | | BRITO, L. G.; OLIVEIRA, M. C. de S.; FOIL, L. D.; GUERRERO, F. D.; LEON, A. P. de; BARBIERI, F. da S.; CHAGAS, A. C. de S.; BARROS, A. T. M. de. Characterization of pesticede resistance in horn fly populations as a risk for fixation fo restance alles in cattle tick population. In: SIMPÓSIO EMBRAPA LABEX EUA DE SANIDADE ANIMAL, 2., 2012, Brasília, DF. Anais... Brasília: Embrapa Estudos e Capacitação, 2012. p.129-130| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 18. | | BRITO, L. G.; OLIVEIRA, M. C. de S.; FOIL, L. D.; GUERRERO, F. D.; PEREZ DE LEON, A.; BARBIERI, F. da S.; CHAGAS, A. C. de S.; BARROS, A. T. M. de. Characterization of pesticide in horn fly populations as a risk factor for fixation of resistance alleles in cattle tick populations. In: SIMPÓSIO EMBRAPA LABEX EUA DE SANIDADE ANIMAL, 2., Brasília, 2012. Proceedings... Brasília: Embrapa Estudos e Capacitação, 2012. p. 129| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pantanal. |
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| 19. | | VIOL, M. A.; GUERRERO, F. D.; OLIVEIRA, B. C. M. de; AQUINO, M. C. C. de; LOIOLA, S. H.; MELO, G. D. de; GOMES, A. H. de S.; KANAMURA, C. T.; GARCIA, M. V.; ANDREOTTI, R.; LIMA, V. M. F. de; BRESCIANI, K. D. S. Identification of Leishmania spp. promastigotes in the intestines, ovaries, and salivary glands of Rhipicephalus sanguineus actively infesting dogs. Parasitology Research, v. 115, n, 9, p. 3479-3484, sept. 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Gado de Corte. |
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| Registros recuperados : 19 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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