Data/hora: 25/06/2024 05:33:37

Biblioteca(s): | Embrapa Café; Embrapa Unidades Centrais.
Data corrente: | 22/02/2011
Data da última atualização: | 09/01/2024
Autoria: | MALUF, M. P.; SILVA, C. C. da; OLIVEIRA, M. de P. A. de; TAVARES, A. G.; SILVAROLLA, M. B.; GUERREIRO FILHO, O.
Afiliação: | MIRIAN PEREZ MALUF, SAPC; CARLA CRISTINA DA SILVA, INSTITUTO AGRONÔMICO, CENTRO DE CAFE 'ALCIDES CARVALHO'; MICHELLE DE PAULA ABREU DE OLIVEIRA, INSTITUTO AGRONÔMICO, CENTRO DE CAFE 'ALCIDES CARVALHO'; ALINE GOMES TAVARES, INSTITUTO AGRONÔMICO, CENTRO DE CAFE 'ALCIDES CARVALHO'; MARIA BERNADETE SILVAROLLA, INSTITUTO AGRONÔMICO, CENTRO DE CAFE 'ALCIDES CARVALHO'; OLIVEIRO GUERREIRO FILHO, INSTITUTO AGRONÔMICO, CENTRO DE CAFE 'ALCIDES CARVALHO'.
Título: | Altered expression of the caffeine synthase gene in a naturally caffeine-free mutant of Coffea arabica.
Ano de publicação: | 2009
Fonte/Imprenta: | Genetics and Molecular Biology, v. 34, n. 2, p. 802-810, 2009.
Idioma: | Inglês
Conteúdo: | In this work, we studied the biosynthesis of caffeine by examining the expression of genes involved in this biosynthetic pathway in coffee fruits containing normal or low levels of this substance. The amplification of gene-specific transcripts during fruit development revealed that low-caffeine fruits had a lower expression of the theobromine synthase and caffeine synthase genes and also contained an extra transcript of the caffeine synthase gene. This extra transcript contained only part of exon 1 and all of exon 3. The sequence of the mutant caffeine synthase gene revealed the substitution of isoleucine for valine in the enzyme active site that probably interfered with enzymatic activity. These findings indicate that the absence of caffeine in these mutants probably resulted from a combination of transcriptional regulation and the presence of mutations in the caffeine synthase amino acid sequence.
Palavras-Chave: | Coffee; Cup quality; Differential expression; Naturally decaffeinated.
Categoria do assunto: | --
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/29273/1/Altered-expression.pdf
Registro original: | Embrapa Café (CNPCa)

Exemplares:

AI-SEDE - (49030-1) - EMB - AP - DD -  -  -  - DIS
CNPCa - (69-1) - UPC - AP - DD -  -  -  - DIS