Data/hora: 21/06/2024 07:31:46

Biblioteca(s): | Embrapa Arroz e Feijão.
Data corrente: | 29/10/2004
Data da última atualização: | 04/11/2004
Autoria: | GUERRA, M. P.; ROGALSKI, M.; SILVA, A. L. da.
Título: | Embryo culture and in vitro multiplication of Prunus 'Capdeboscq' rootstock.
Ano de publicação: | 2003
Fonte/Imprenta: | Crop Breeding and Applied Biotechnology, Londrina, v. 3, n. 2, p. 141-147, June 2003.
Idioma: | Inglês
Conteúdo: | Embryo culture and recovery are techniques used in basic and applied studies of morphogenesis, in vitro propagation and genetic in the Prunus genus. The present study assessed the germination and organogenetic development of embryos in culture media with different concentrations of GA3 as well as the in vitro multiplication rate of clones of the 'Capdeboscq' rootstock. Embryonic axes were excised and inoculated in Lepoivre culture medium supplemented with BAP (0.1 mg L-1), NAA (0.1 mg L-1) and GA3 (0.0; 1.0; 2.5; 5.0;7.5 and 10.0 mg L-1). The in vitro multiplication  potential of four clones was assessed after embryo germination using the double phase multiplication method in the last subculture. The results showed a mean of 70.7% in vitro germination. GA3 was not effective in the germination, but induced greater elongation of the embryo and inhibited the root development. The clones obtained in the embryo culture showed high potential for in vitro multiplication with rates of 6.9 and 14.9 shoots per explant.
Palavras-Chave: | Cultura; In vitro propagation; Peach tree; Porta-enxerto.
Thesagro: | Embrião.
Thesaurus Nal: | germination; gibberellic acid; organogenesis.
Categoria do assunto: | --
Registro original: | Embrapa Arroz e Feijão (CNPAF)
Exemplares: | CNPAF - (21809-1) - ADD - AP - -- - 631.05 - C - 2004.00157 - DIS