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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
21/02/2014 |
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Data da última atualização: |
05/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
POLISSENI, J.; GRÁZIB, J. G. V. de; GUERRA, M. DE O.; MILEN, L. C.; CAMARGO, L. S. de A.; PETERS, V. M. |
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Afiliação: |
JULIANA POLISSENI, UFJF; JOÃO GABRIEL VIANA DE GRÁZIB, UFJF; MARTHA DE OLIVEIRA GUERRA, UFJF; LARISSA CABRAL MILEN, UFJF; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; VERA MARIA PETERS, UFJF. |
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Título: |
Impact of the use of gonadotropins on embryonic development - a rat model. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
JBRA Assisted Reproduction, v. 17, n. 1, p. 32-36, 2013. |
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Idioma: |
Inglês |
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Conteúdo: |
Objective: To evaluate the impact of in vivo superovulation on early embryonic development in a rat model. Methods: Experiment 1: Female Wistar rats were superovulated with injection of PMSG followed by hCG 48 hours later. Embryos were collected 24 h after hCG administration. Experiment 2: Female Wistar rats (n=16) were distrbuted to a control or superovulated group. Embryos were collected 48 and 72 h after hCG administration. The total number of embryonic structures and the embryo quality grades were analyzed by Chi-squared test. Between-group differences in ovarian weight were assessed by Student’s t-test. Results: Experiment 1: 931 embryos were collected from 66 animals, with a mean of 16.3±9.5 structures per animal. The superovulation was successful in 89.4% of the animals, and 98.2% of the embryos collected from superovulated animals were viable. Experiment 2: The superovulated group had heavier ovaries and produced more embryos than the control group (P < 0.001); however, these embryos contained fewer cells than controls at 72 hours after hCG administration (P<0.001). A greater percentage of embryos from the superovulated group were classified as grade I or grade II relative to embryos from the control group (P < 0.001 and P = 0.008, respectively). Conclusion: Superovulation was successful in the Wistar rat model and resulted in a greater number of embryos collected per animal. However, embryos produced as a result of superovulation developed more slowly than control embryos. MenosObjective: To evaluate the impact of in vivo superovulation on early embryonic development in a rat model. Methods: Experiment 1: Female Wistar rats were superovulated with injection of PMSG followed by hCG 48 hours later. Embryos were collected 24 h after hCG administration. Experiment 2: Female Wistar rats (n=16) were distrbuted to a control or superovulated group. Embryos were collected 48 and 72 h after hCG administration. The total number of embryonic structures and the embryo quality grades were analyzed by Chi-squared test. Between-group differences in ovarian weight were assessed by Student’s t-test. Results: Experiment 1: 931 embryos were collected from 66 animals, with a mean of 16.3±9.5 structures per animal. The superovulation was successful in 89.4% of the animals, and 98.2% of the embryos collected from superovulated animals were viable. Experiment 2: The superovulated group had heavier ovaries and produced more embryos than the control group (P < 0.001); however, these embryos contained fewer cells than controls at 72 hours after hCG administration (P<0.001). A greater percentage of embryos from the superovulated group were classified as grade I or grade II relative to embryos from the control group (P < 0.001 and P = 0.008, respectively). Conclusion: Superovulation was successful in the Wistar rat model and resulted in a greater number of embryos collected per animal. However, embryos produced as a result of superovulation developed more slowly than control e... Mostrar Tudo |
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Palavras-Chave: |
Animal model; Embryo development; Embryo grade. |
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Thesaurus Nal: |
superovulation. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02182naa a2200229 a 4500
001 1980786
005 2024-02-05
008 2013 bl uuuu u00u1 u #d
100 1 $aPOLISSENI, J.
245 $aImpact of the use of gonadotropins on embryonic development - a rat model.$h[electronic resource]
260 $c2013
520 $aObjective: To evaluate the impact of in vivo superovulation on early embryonic development in a rat model. Methods: Experiment 1: Female Wistar rats were superovulated with injection of PMSG followed by hCG 48 hours later. Embryos were collected 24 h after hCG administration. Experiment 2: Female Wistar rats (n=16) were distrbuted to a control or superovulated group. Embryos were collected 48 and 72 h after hCG administration. The total number of embryonic structures and the embryo quality grades were analyzed by Chi-squared test. Between-group differences in ovarian weight were assessed by Student’s t-test. Results: Experiment 1: 931 embryos were collected from 66 animals, with a mean of 16.3±9.5 structures per animal. The superovulation was successful in 89.4% of the animals, and 98.2% of the embryos collected from superovulated animals were viable. Experiment 2: The superovulated group had heavier ovaries and produced more embryos than the control group (P < 0.001); however, these embryos contained fewer cells than controls at 72 hours after hCG administration (P<0.001). A greater percentage of embryos from the superovulated group were classified as grade I or grade II relative to embryos from the control group (P < 0.001 and P = 0.008, respectively). Conclusion: Superovulation was successful in the Wistar rat model and resulted in a greater number of embryos collected per animal. However, embryos produced as a result of superovulation developed more slowly than control embryos.
650 $asuperovulation
653 $aAnimal model
653 $aEmbryo development
653 $aEmbryo grade
700 1 $aGRÁZIB, J. G. V. de
700 1 $aGUERRA, M. DE O.
700 1 $aMILEN, L. C.
700 1 $aCAMARGO, L. S. de A.
700 1 $aPETERS, V. M.
773 $tJBRA Assisted Reproduction$gv. 17, n. 1, p. 32-36, 2013.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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| Registros recuperados : 4 | |
| 2. |  | POLISSENI, J.; GRÁZIB, J. G. V. de; GUERRA, M. DE O.; MILEN, L. C.; CAMARGO, L. S. de A.; PETERS, V. M. Impact of the use of gonadotropins on embryonic development - a rat model. JBRA Assisted Reproduction, v. 17, n. 1, p. 32-36, 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 3 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 3. | | POLISSENI, J.; GUERRA, M. de O.; SERAPIÃO, R. V.; PEREIRA, M. M.; FOLHADELLA, I. M.; SÁ, W. F.; CAMARGO, L. S. A.; VIANA, J. H. M.; WOHLRES-VIANA, S.; BOITÉ, M. C.; PETERS, V. M. Viabilidade da técnica de biopsia em embriões bovinos fecundados in vitro em estádios iniciais do desenvolvimento. Acta Scientiae Veterinariae, v. 35, p. s1192, 2007. Supl. 3. Edição dos anais da 21ª Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Salvador, 2007.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 4. | | POLISSENI, J.; SERAPIÃO, R. V.; CAMPOS JR. P. H. A.; GRAZIA, J. G. V. DE; MILEN, L. C.; CAMARGO, L. S. de A.; GUERRA, M. DE O.; PETERS, V. M. Tecnica de TUNEL em embriões de ratas Wistar: avaliação da qualidade e da capacidade de desenvolvimento dos embriões. Revista Interdisciplinar de Estudos Experimentais, v. 1, n. 3, p. 110-113, 2010.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: C - 0 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| Registros recuperados : 4 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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