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Registro Completo |
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
27/05/2009 |
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Data da última atualização: |
05/06/2018 |
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Autoria: |
CANGAHUALA-INOCENTE, G. C.; STEINER, N.; MALDONADO, S. B.; GUERRA, M. P. |
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Afiliação: |
Gabriela Claudia Cangahuala?Inocente, Universidade Federal de Santa Catarina, Laboratório de Fisiologia do Desenvolvimento e Genética Vegetal, Programa de Pós?graduação em Recursos Genéticos Vegetais; Neusa Steiner, Universidade Federal de Santa Catarina, Laboratório de Fisiologia do Desenvolvimento e Genética Vegetal, Programa de Pós?graduação em Recursos Genéticos Vegetais; Sara Beatriz Maldonado, Universidad de Buenos Aires, Departamento de Biodiversidad y Biología Experimental; Miguel Pedro Guerra, Universidade Federal de Santa Catarina, Laboratório de Fisiologia do Desenvolvimento e Genética Vegetal, Programa de Pós?graduação em Recursos Genéticos Vegetais. |
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Título: |
Patterns of protein and carbohydrate accumulation during somatic embryogenesis of Acca sellowiana. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, Brasília, DF, v. 44, n. 3, p. 217-224, mar. 2009. |
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Idioma: |
Inglês |
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Notas: |
Título em português: Padrões de acúmulo de proteínas e carboidratos durante a embriogênese somática de Acca sellowiana. |
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Conteúdo: |
The aim of this work was to quantify the protein, starch and total sugars levels during histodifferentiation and development of somatic embryos of Acca sellowiana Berg. For histological observations, the samples were dehydrated in a battery of ethanol, embedded in historesin and stained with toluidine blue (morphology), coomassie blue (protein bodies) and periodic acid?Schiff (starch). Proteins were extracted using a buffer solution, precipitated using ethanol and quantified using the Bradford reagent. Total sugars were extracted using a methanol-chloroform-water (12:5:3) solution and quantified by a reaction with anthrone at 0.2%. Starch was extracted using a 30% perchloric acid solution and quantified by a reaction with anthrone at 0.2%. During the somatic embryogenesis' in vitro morphogenesis and differentiation processes, the total protein levels decreased and the soluble sugars levels increased during the first 30 days in culture and remained stable until the 120th day. On the other hand, total protein levels increased according to the progression in the developmental stages of the somatic embryos. The levels of total sugars and starch increased in the heart and cotyledonary stages, and decreased in the torpedo and pre-cotyledonary stages. These compounds play a central role in the development of somatic embryos of Acca sellowiana. |
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Palavras-Chave: |
açúcares totais; goiabeira serrana; pineapple guava; total sugars. |
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Thesagro: |
Amido; Metabolismo. |
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Thesaurus Nal: |
metabolism; starch. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/38043/1/44n03a01.pdf
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Marc: |
LEADER 02241naa a2200265 a 4500
001 1125724
005 2018-06-05
008 2009 bl uuuu u00u1 u #d
100 1 $aCANGAHUALA-INOCENTE, G. C.
245 $aPatterns of protein and carbohydrate accumulation during somatic embryogenesis of Acca sellowiana.
260 $c2009
500 $aTítulo em português: Padrões de acúmulo de proteínas e carboidratos durante a embriogênese somática de Acca sellowiana.
520 $aThe aim of this work was to quantify the protein, starch and total sugars levels during histodifferentiation and development of somatic embryos of Acca sellowiana Berg. For histological observations, the samples were dehydrated in a battery of ethanol, embedded in historesin and stained with toluidine blue (morphology), coomassie blue (protein bodies) and periodic acid?Schiff (starch). Proteins were extracted using a buffer solution, precipitated using ethanol and quantified using the Bradford reagent. Total sugars were extracted using a methanol-chloroform-water (12:5:3) solution and quantified by a reaction with anthrone at 0.2%. Starch was extracted using a 30% perchloric acid solution and quantified by a reaction with anthrone at 0.2%. During the somatic embryogenesis' in vitro morphogenesis and differentiation processes, the total protein levels decreased and the soluble sugars levels increased during the first 30 days in culture and remained stable until the 120th day. On the other hand, total protein levels increased according to the progression in the developmental stages of the somatic embryos. The levels of total sugars and starch increased in the heart and cotyledonary stages, and decreased in the torpedo and pre-cotyledonary stages. These compounds play a central role in the development of somatic embryos of Acca sellowiana.
650 $ametabolism
650 $astarch
650 $aAmido
650 $aMetabolismo
653 $aaçúcares totais
653 $agoiabeira serrana
653 $apineapple guava
653 $atotal sugars
700 1 $aSTEINER, N.
700 1 $aMALDONADO, S. B.
700 1 $aGUERRA, M. P.
773 $tPesquisa Agropecuária Brasileira, Brasília, DF$gv. 44, n. 3, p. 217-224, mar. 2009.
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Embrapa Unidades Centrais (AI-SEDE) |
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| 19. |  | NODARI, R. O.; REIS, A.; GUERRA, M. P.; REIS, M. S. dos; FLORIANO, E. P. Analise preliminar do inventario do palmiteiro em floresta ombrofila densa montana. In: ENCONTRO NACIONAL DE PESQUISADORES DE PALMITO, 1., 1987, Curitiba. Anais. Curitiba: EMBRAPA-CNPF, 1988. p.159-164. (EMBRAPA-CNPF. Documentos, 19).| Biblioteca(s): Embrapa Florestas. |
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| 20. | | PINTO, A. A.; COELHO, M. C. F.; SOUZA JÚNIOR, M. T.; GUERRA, M. P. Expressão transiente do Gene gus em diferentes tecidos de mamoeiro (Carica papaya L.) e videira (Vitis sp.) In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 6., 2001, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2001. p. 37.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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