|
Registro Completo |
|
Biblioteca(s): |
Embrapa Milho e Sorgo. |
|
Data corrente: |
30/03/2016 |
|
Data da última atualização: |
28/04/2016 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
LEITE, N. A.; MENDES, S. M.; SANTOS-AMAYA, O. F.; SANTOS, C. A.; TEIXEIRA, T. P. M.; GUEDES, R. N. C.; PEREIRA, E. J. G. |
|
Afiliação: |
Natália A. Leite, Universidade Federal de Viçosa; SIMONE MARTINS MENDES, CNPMS; Oscar F. Santos-Amaya, Universidade Federal de Viçosa; Chritiane A. Santos, Universidade Federal de Viçosa; Thaís P. M. Teixeira, Universidade Federal de Viçosa; Raul N. C. Guedes, Universidade Federal de Viçosa; Eliseu J. G. Pereira, Universidade Federal de Viçosa. |
|
Título: |
Rapid selection and characterization of Cry1F resistance in a Brazilian strain of fall armyworm. |
|
Ano de publicação: |
2016 |
|
Fonte/Imprenta: |
Entomologia Experimentalis et Applicata, Dordrecht, v. 158, p. 236-247, 2016. |
|
DOI: |
10.1111/eea.12399 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Transgenic maize (Zea mays L., Poaceae) event TC1507, producing the Cry1F protein of Bacillus thuringiensis Berliner, has been used for management of the fall armyworm, Spodoptera frugiperda (JE Smith) (Lepidoptera: Noctuidae), in Brazil since 2009. A strain of S. frugiperda, obtained from field collections of larvae in TC1507 maize in Minas Gerais state in 2010, was selected in the laboratory for resistance to Cry1F using leaves of TC1507 maize in two selection regimes. Continuous exposure of larvae to Cry1F was more effective than exposure for 6, 8, and 10 days in the selection of resistant S. frugiperda individuals. With only four generations of laboratory selection, a strain with high levels of resistance to Cry1F was obtained, as indicated by the survival of insects reared on leaves of TC1507 maize plants and by the more than 300-fold resistance level measured in bioassays with the purified Cry1F protein. Importantly, reciprocal crosses between control and the Cry1F-selected strains revealed that the resistance is autosomal and incompletely recessive, and the response obtained in the backcross of the F1 generation with the resistant strain was consistent with simple monogenic inheritance. Additionally, there were no apparent fitness costs associated with resistance either for survival or larval growth on non-Bt maize leaves. Our findings provide experimental evidence for rapid evolution of Cry1F resistance in S. frugiperda in the laboratory and further reinforce the potential of this species to evolve field resistance to the TC1507 maize as previously reported. The resistant strain isolated in this study provides an opportunity to estimate the resistance allele frequency in the field and to determine the biochemical and molecular basis of the resistance, which should provide further information to assist in the resistance management of S. frugiperda on transgenicmaize producing B. thuringiensis proteins. MenosTransgenic maize (Zea mays L., Poaceae) event TC1507, producing the Cry1F protein of Bacillus thuringiensis Berliner, has been used for management of the fall armyworm, Spodoptera frugiperda (JE Smith) (Lepidoptera: Noctuidae), in Brazil since 2009. A strain of S. frugiperda, obtained from field collections of larvae in TC1507 maize in Minas Gerais state in 2010, was selected in the laboratory for resistance to Cry1F using leaves of TC1507 maize in two selection regimes. Continuous exposure of larvae to Cry1F was more effective than exposure for 6, 8, and 10 days in the selection of resistant S. frugiperda individuals. With only four generations of laboratory selection, a strain with high levels of resistance to Cry1F was obtained, as indicated by the survival of insects reared on leaves of TC1507 maize plants and by the more than 300-fold resistance level measured in bioassays with the purified Cry1F protein. Importantly, reciprocal crosses between control and the Cry1F-selected strains revealed that the resistance is autosomal and incompletely recessive, and the response obtained in the backcross of the F1 generation with the resistant strain was consistent with simple monogenic inheritance. Additionally, there were no apparent fitness costs associated with resistance either for survival or larval growth on non-Bt maize leaves. Our findings provide experimental evidence for rapid evolution of Cry1F resistance in S. frugiperda in the laboratory and further reinforce the pot... Mostrar Tudo |
|
Palavras-Chave: |
Lagarta do cartucho; Manejo de resistência; Milho transgênico Bt. |
|
Thesagro: |
Bacillus thuringiensis; Praga de planta; Spodoptera frugiperda; Zea mays. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 02845naa a2200289 a 4500
001 2042142
005 2016-04-28
008 2016 bl uuuu u00u1 u #d
024 7 $a10.1111/eea.12399$2DOI
100 1 $aLEITE, N. A.
245 $aRapid selection and characterization of Cry1F resistance in a Brazilian strain of fall armyworm.$h[electronic resource]
260 $c2016
520 $aTransgenic maize (Zea mays L., Poaceae) event TC1507, producing the Cry1F protein of Bacillus thuringiensis Berliner, has been used for management of the fall armyworm, Spodoptera frugiperda (JE Smith) (Lepidoptera: Noctuidae), in Brazil since 2009. A strain of S. frugiperda, obtained from field collections of larvae in TC1507 maize in Minas Gerais state in 2010, was selected in the laboratory for resistance to Cry1F using leaves of TC1507 maize in two selection regimes. Continuous exposure of larvae to Cry1F was more effective than exposure for 6, 8, and 10 days in the selection of resistant S. frugiperda individuals. With only four generations of laboratory selection, a strain with high levels of resistance to Cry1F was obtained, as indicated by the survival of insects reared on leaves of TC1507 maize plants and by the more than 300-fold resistance level measured in bioassays with the purified Cry1F protein. Importantly, reciprocal crosses between control and the Cry1F-selected strains revealed that the resistance is autosomal and incompletely recessive, and the response obtained in the backcross of the F1 generation with the resistant strain was consistent with simple monogenic inheritance. Additionally, there were no apparent fitness costs associated with resistance either for survival or larval growth on non-Bt maize leaves. Our findings provide experimental evidence for rapid evolution of Cry1F resistance in S. frugiperda in the laboratory and further reinforce the potential of this species to evolve field resistance to the TC1507 maize as previously reported. The resistant strain isolated in this study provides an opportunity to estimate the resistance allele frequency in the field and to determine the biochemical and molecular basis of the resistance, which should provide further information to assist in the resistance management of S. frugiperda on transgenicmaize producing B. thuringiensis proteins.
650 $aBacillus thuringiensis
650 $aPraga de planta
650 $aSpodoptera frugiperda
650 $aZea mays
653 $aLagarta do cartucho
653 $aManejo de resistência
653 $aMilho transgênico Bt
700 1 $aMENDES, S. M.
700 1 $aSANTOS-AMAYA, O. F.
700 1 $aSANTOS, C. A.
700 1 $aTEIXEIRA, T. P. M.
700 1 $aGUEDES, R. N. C.
700 1 $aPEREIRA, E. J. G.
773 $tEntomologia Experimentalis et Applicata, Dordrecht$gv. 158, p. 236-247, 2016.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
|
