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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Soja. |
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Data corrente: |
18/06/2012 |
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Data da última atualização: |
03/03/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GUIMARÃES-DIAS, F.; NEVES-BORGES, A. C.; VIANA, A. A. B.; MESQUITA, R. O.; ROMANO, E.; GROSSI-DE-SÁ, M. de F.; NEPOMUCENO, A. L.; LOUREIRO, M. E.; ALVES-FERREIRA, M. |
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Afiliação: |
FÁBIA GUIMARÃES DIAS, UFRJ; ANNA CRISTINA NEVES-BORGES, UNIRIO; ANTONIO AMERICO BARBOSA VIANA, CENARGEN; ROSILENE OLIVEIRA MESQUITA, UFV; EDUARDO ROMANO DE CAMPOS PINTO, CENARGEN; MARIA FATIMA GROSSI DE SA, CENARGEN; ALEXANDRE LIMA NEPOMUCENO, SRI; MARCELO EHLERS LOUREIRO, UFV; MÁRCIO ALVES-FERREIRA, UFRJ. |
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Título: |
Expression analysis in response to drought stress in soybean: shedding light on the regulation of metabolic pathway genes. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Genetics and Molecular Biology, Ribeirão Preto, v. 35, n. 1, suppl., p. 222-232, May 2012. |
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Idioma: |
Inglês |
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Conteúdo: |
Metabolomics analysis of wild type Arabidopsis thaliana plants, under control and drought stress conditions revealed several metabolic pathways that are induced under water deficit. The metabolic response to drought stress is also associated with ABA dependent and independent pathways, allowing a better understanding of the molecular mechanisms in this model plant. Through combining an in silico approach and gene expression analysis by quantitative real-time PCR, the present work aims at identifying genes of soybean metabolic pathways potentially associated with water deficit. Digital expression patterns of Arabidopsis genes, which were selected based on the basis of literature reports, were evaluated under drought stress condition by Genevestigator. Genes that showed strong induction under drought stress were selected and used as bait to identify orthologs in the soybean genome. This allowed us to select 354 genes of putative soybean orthologs of 79 Arabidopsis genes belonging to 38 distinct metabolic pathways. The expression pattern of the selected genes was verified in the subtractive libraries available in the GENOSOJA project. Subsequently, 13 genes from different metabolic pathways were selected for validation by qPCR experiments. The expression of six genes was validated in plants undergoing drought stress in both pot-based and hydroponic cultivation systems. The results suggest that the metabolic response to drought stress is conserved in Arabidopsis and soybean plants. MenosMetabolomics analysis of wild type Arabidopsis thaliana plants, under control and drought stress conditions revealed several metabolic pathways that are induced under water deficit. The metabolic response to drought stress is also associated with ABA dependent and independent pathways, allowing a better understanding of the molecular mechanisms in this model plant. Through combining an in silico approach and gene expression analysis by quantitative real-time PCR, the present work aims at identifying genes of soybean metabolic pathways potentially associated with water deficit. Digital expression patterns of Arabidopsis genes, which were selected based on the basis of literature reports, were evaluated under drought stress condition by Genevestigator. Genes that showed strong induction under drought stress were selected and used as bait to identify orthologs in the soybean genome. This allowed us to select 354 genes of putative soybean orthologs of 79 Arabidopsis genes belonging to 38 distinct metabolic pathways. The expression pattern of the selected genes was verified in the subtractive libraries available in the GENOSOJA project. Subsequently, 13 genes from different metabolic pathways were selected for validation by qPCR experiments. The expression of six genes was validated in plants undergoing drought stress in both pot-based and hydroponic cultivation systems. The results suggest that the metabolic response to drought stress is conserved in Arabidopsis and soybean plan... Mostrar Tudo |
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Palavras-Chave: |
Bioinformática; QPCR. |
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Thesagro: |
Gene; Genoma; Resistência a seca; Soja. |
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Thesaurus Nal: |
Biochemical pathways; Bioinformatics; Drought tolerance; Genes; Genome; Soybeans. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/61069/1/gmb.expression.anal.v35n1s.222-232.2012.pdf
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Marc: |
LEADER 02539naa a2200361 a 4500
001 1946634
005 2023-03-03
008 2012 bl uuuu u00u1 u #d
100 1 $aGUIMARÃES-DIAS, F.
245 $aExpression analysis in response to drought stress in soybean$bshedding light on the regulation of metabolic pathway genes.
260 $c2012
520 $aMetabolomics analysis of wild type Arabidopsis thaliana plants, under control and drought stress conditions revealed several metabolic pathways that are induced under water deficit. The metabolic response to drought stress is also associated with ABA dependent and independent pathways, allowing a better understanding of the molecular mechanisms in this model plant. Through combining an in silico approach and gene expression analysis by quantitative real-time PCR, the present work aims at identifying genes of soybean metabolic pathways potentially associated with water deficit. Digital expression patterns of Arabidopsis genes, which were selected based on the basis of literature reports, were evaluated under drought stress condition by Genevestigator. Genes that showed strong induction under drought stress were selected and used as bait to identify orthologs in the soybean genome. This allowed us to select 354 genes of putative soybean orthologs of 79 Arabidopsis genes belonging to 38 distinct metabolic pathways. The expression pattern of the selected genes was verified in the subtractive libraries available in the GENOSOJA project. Subsequently, 13 genes from different metabolic pathways were selected for validation by qPCR experiments. The expression of six genes was validated in plants undergoing drought stress in both pot-based and hydroponic cultivation systems. The results suggest that the metabolic response to drought stress is conserved in Arabidopsis and soybean plants.
650 $aBiochemical pathways
650 $aBioinformatics
650 $aDrought tolerance
650 $aGenes
650 $aGenome
650 $aSoybeans
650 $aGene
650 $aGenoma
650 $aResistência a seca
650 $aSoja
653 $aBioinformática
653 $aQPCR
700 1 $aNEVES-BORGES, A. C.
700 1 $aVIANA, A. A. B.
700 1 $aMESQUITA, R. O.
700 1 $aROMANO, E.
700 1 $aGROSSI-DE-SÁ, M. de F.
700 1 $aNEPOMUCENO, A. L.
700 1 $aLOUREIRO, M. E.
700 1 $aALVES-FERREIRA, M.
773 $tGenetics and Molecular Biology, Ribeirão Preto$gv. 35, n. 1, suppl., p. 222-232, May 2012.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Meio Ambiente. Para informações adicionais entre em contato com cnpma.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
28/02/2013 |
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Data da última atualização: |
28/02/2013 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
CABRAL, O. M. R.; ROCHA, H. R. da; GASH, J. H.; LIGO, M. A. V.; TATSCH, J. D.; FREITAS, H. C. de; BRASILIO, E. |
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Afiliação: |
OSVALDO MACHADO RODRIGUES CABRAL, CNPMA; HUMBERTO R. ROCHA, USP; JOHN H. GASH, Centre for Ecology and Hydrology; MARCOS ANTONIO VIEIRA LIGO, CNPMA; J O N A T A N D . T A T S C H, USP; H E L B E R C . F R E I T A S, USP; E M I L I A B R A S I L I O, USP. |
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Título: |
Water use in a sugarcane plantation. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Global Change Biology. Bioenergy, Oxford, v. 4, p. 555-565, 2012. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: The evapotranspiration (E) from a sugarcane plantation in the southeast Brazil was measured by the eddy-covariance method during two consecutive cycles. These represented the second (393 days) and third year (374 days) re-growth (ratoon). The total E in the first cycle was 829 mm, accounting for 69% of rainfall, whereas in the second cycle, it was 690 mm, despite the total rainfall (1353 mm) being 13% greater. The ratio of E to available energy, the evaporative fraction, exhibited a smaller variation between the first and second cycles: 0.58 and 0.51, respectively. The estimated interception losses were 88 and 90 mm, respectively, accounting for approximately 7% of the total rainfall. The sugarcane yield in the second cycle (61.5 ± 4.0 t ha -¹) was 26% lower than the first cycle, as well as lower than the regional average for the third ratoon (76 t ha -¹). The below average yield was associated with less available soil water at the beginning of the cycle, with the amount of rainfall recorded during the first 120 days of re-growth in the second cycle being 16% of that recorded in the first (203 mm). |
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Palavras-Chave: |
Rainfall interception. |
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Thesagro: |
Água; Cana de açúcar; Evapotranspiração. |
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Thesaurus NAL: |
eddy covariance; Evapotranspiration; Soil water deficit; Sugarcane. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01920naa a2200289 a 4500
001 1951600
005 2013-02-28
008 2012 bl uuuu u00u1 u #d
100 1 $aCABRAL, O. M. R.
245 $aWater use in a sugarcane plantation.$h[electronic resource]
260 $c2012
520 $aAbstract: The evapotranspiration (E) from a sugarcane plantation in the southeast Brazil was measured by the eddy-covariance method during two consecutive cycles. These represented the second (393 days) and third year (374 days) re-growth (ratoon). The total E in the first cycle was 829 mm, accounting for 69% of rainfall, whereas in the second cycle, it was 690 mm, despite the total rainfall (1353 mm) being 13% greater. The ratio of E to available energy, the evaporative fraction, exhibited a smaller variation between the first and second cycles: 0.58 and 0.51, respectively. The estimated interception losses were 88 and 90 mm, respectively, accounting for approximately 7% of the total rainfall. The sugarcane yield in the second cycle (61.5 ± 4.0 t ha -¹) was 26% lower than the first cycle, as well as lower than the regional average for the third ratoon (76 t ha -¹). The below average yield was associated with less available soil water at the beginning of the cycle, with the amount of rainfall recorded during the first 120 days of re-growth in the second cycle being 16% of that recorded in the first (203 mm).
650 $aeddy covariance
650 $aEvapotranspiration
650 $aSoil water deficit
650 $aSugarcane
650 $aÁgua
650 $aCana de açúcar
650 $aEvapotranspiração
653 $aRainfall interception
700 1 $aROCHA, H. R. da
700 1 $aGASH, J. H.
700 1 $aLIGO, M. A. V.
700 1 $aTATSCH, J. D.
700 1 $aFREITAS, H. C. de
700 1 $aBRASILIO, E.
773 $tGlobal Change Biology. Bioenergy, Oxford$gv. 4, p. 555-565, 2012.
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Embrapa Meio Ambiente (CNPMA) |
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