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 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
01/09/2015 |
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Data da última atualização: |
29/03/2016 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
OLIVEIRA, M. B.; LOBO JUNIOR, M.; GROSSI-DE-SÁ, M. F.; PETROFEZA, S. |
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Afiliação: |
MARÍLIA BARROS OLIVEIRA, UFG; MURILLO LOBO JUNIOR, CNPAF; MARIA FATIMA GROSSI DE SA, CENARGEN; SILVANA PETROFEZA, UFG. |
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Título: |
Exogenous application of methyl jasmonate induces a defense response and resistance against Sclerotinia sclerotiorum in dry bean plants. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Journal of Plant Physiology, v. 182, p. 13-22, June 2015. |
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DOI: |
10.1016/j.jplph.2015.04.006 |
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Idioma: |
Inglês |
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Conteúdo: |
Sclerotinia sclerotiorum (Lib.) de Bary is a necrotrophic fungal pathogen that causes a disease known as white mold, which is a major problem for dry bean (Phaseolus vulgaris L.) and other crops in many growing areas in Brazil. To investigate the role of methyl jasmonate (MeJA) in defending dry bean plants against S. sclerotiorum, we used suppression subtractive hybridization (SSH) of cDNA and identified genes that are differentially expressed during plant?pathogen interactions after treatment. Exogenous MeJA application enhanced resistance to the pathogen, and SSH analyses led to the identification of 94 unigenes, presumably involved in a variety of functions, which were classified into several functional categories, including metabolism, signal transduction, protein biogenesis and degradation, and cell defense and rescue. Using RT?qPCR, some unigenes were found to be differentially expressed in a time-dependent manner in dry bean plants during the interaction with S. sclerotiorum after MeJA treatment, including the pathogenes is related protein PR3 (chitinase), PvCallose (callose synthase), PvNBS-LRR (NBS-LRR resistance-like protein), PvF-box (F-box family protein-like), and a polygalacturonase inhibitor protein (PGIP). Based on these expression data, the putative roles of differentially expressed genes were discussed in relation to the disease and MeJA resistance induction. Changes in the activity of the pathogenesis-related proteins - 1,3-glucanase, chitinase, phenylalanine ammonia-lyase, and peroxidase in plants after MeJA treatment and following inoculation of the pathogen were also investigated as molecular markers of induced resistance. Foliar application of MeJA induced partial resistance against S. sclerotiorum in plants as well as a consistent increase in pathogenesis-related protein activities. Our findings provide new insights into the physiological and molecular mechanisms of resistance induced by MeJA in the P. vulgaris - S. sclerotiorum pathosystem. MenosSclerotinia sclerotiorum (Lib.) de Bary is a necrotrophic fungal pathogen that causes a disease known as white mold, which is a major problem for dry bean (Phaseolus vulgaris L.) and other crops in many growing areas in Brazil. To investigate the role of methyl jasmonate (MeJA) in defending dry bean plants against S. sclerotiorum, we used suppression subtractive hybridization (SSH) of cDNA and identified genes that are differentially expressed during plant?pathogen interactions after treatment. Exogenous MeJA application enhanced resistance to the pathogen, and SSH analyses led to the identification of 94 unigenes, presumably involved in a variety of functions, which were classified into several functional categories, including metabolism, signal transduction, protein biogenesis and degradation, and cell defense and rescue. Using RT?qPCR, some unigenes were found to be differentially expressed in a time-dependent manner in dry bean plants during the interaction with S. sclerotiorum after MeJA treatment, including the pathogenes is related protein PR3 (chitinase), PvCallose (callose synthase), PvNBS-LRR (NBS-LRR resistance-like protein), PvF-box (F-box family protein-like), and a polygalacturonase inhibitor protein (PGIP). Based on these expression data, the putative roles of differentially expressed genes were discussed in relation to the disease and MeJA resistance induction. Changes in the activity of the pathogenesis-related proteins - 1,3-glucanase, chitinase, phenylalan... Mostrar Tudo |
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Palavras-Chave: |
Genes relacionadas com patogénese. |
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Thesagro: |
Doença de planta; Feijão; Fungo; Mofo Branco; Phaseolus vulgaris; Sclerotinia sclerotiorum. |
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Thesaurus Nal: |
Dry beans; Methyl jasmonate. |
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Categoria do assunto: |
--
H Saúde e Patologia |
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Marc: |
LEADER 02883naa a2200277 a 4500
001 2025021
005 2016-03-29
008 2015 bl uuuu u00u1 u #d
024 7 $a10.1016/j.jplph.2015.04.006$2DOI
100 1 $aOLIVEIRA, M. B.
245 $aExogenous application of methyl jasmonate induces a defense response and resistance against Sclerotinia sclerotiorum in dry bean plants.$h[electronic resource]
260 $c2015
520 $aSclerotinia sclerotiorum (Lib.) de Bary is a necrotrophic fungal pathogen that causes a disease known as white mold, which is a major problem for dry bean (Phaseolus vulgaris L.) and other crops in many growing areas in Brazil. To investigate the role of methyl jasmonate (MeJA) in defending dry bean plants against S. sclerotiorum, we used suppression subtractive hybridization (SSH) of cDNA and identified genes that are differentially expressed during plant?pathogen interactions after treatment. Exogenous MeJA application enhanced resistance to the pathogen, and SSH analyses led to the identification of 94 unigenes, presumably involved in a variety of functions, which were classified into several functional categories, including metabolism, signal transduction, protein biogenesis and degradation, and cell defense and rescue. Using RT?qPCR, some unigenes were found to be differentially expressed in a time-dependent manner in dry bean plants during the interaction with S. sclerotiorum after MeJA treatment, including the pathogenes is related protein PR3 (chitinase), PvCallose (callose synthase), PvNBS-LRR (NBS-LRR resistance-like protein), PvF-box (F-box family protein-like), and a polygalacturonase inhibitor protein (PGIP). Based on these expression data, the putative roles of differentially expressed genes were discussed in relation to the disease and MeJA resistance induction. Changes in the activity of the pathogenesis-related proteins - 1,3-glucanase, chitinase, phenylalanine ammonia-lyase, and peroxidase in plants after MeJA treatment and following inoculation of the pathogen were also investigated as molecular markers of induced resistance. Foliar application of MeJA induced partial resistance against S. sclerotiorum in plants as well as a consistent increase in pathogenesis-related protein activities. Our findings provide new insights into the physiological and molecular mechanisms of resistance induced by MeJA in the P. vulgaris - S. sclerotiorum pathosystem.
650 $aDry beans
650 $aMethyl jasmonate
650 $aDoença de planta
650 $aFeijão
650 $aFungo
650 $aMofo Branco
650 $aPhaseolus vulgaris
650 $aSclerotinia sclerotiorum
653 $aGenes relacionadas com patogénese
700 1 $aLOBO JUNIOR, M.
700 1 $aGROSSI-DE-SÁ, M. F.
700 1 $aPETROFEZA, S.
773 $tJournal of Plant Physiology$gv. 182, p. 13-22, June 2015.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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Biblioteca |
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Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
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URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Solos. |
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Data corrente: |
19/10/2015 |
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Data da última atualização: |
03/11/2021 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
FRANCHINI, C. A.; OLIVEIRA, T. L.; TEIXEIRA, W. G.; KUZNETSOV, A.; ACHETE, C. A. |
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Afiliação: |
C. A. FRANCHINI, INMETRO; T. L. OLIVEIRA, INMETRO; WENCESLAU GERALDES TEIXEIRA, CNPS; A. KUZNETSOV, INMETRO; C. A. ACHETE, INMETRO/UFRJ. |
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Título: |
Phase separation methodology for physicochemical studies of soils. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Journal of Physics: Conference Series, v. 575, 2015. Edition of the Proceedings of VII Brazilian Congress on Metrology, Ouro Preto, Nov. 2013. Ref. 012037. |
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Idioma: |
Inglês |
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Conteúdo: |
A detailed study of a soil in every country is of a paramount importance, because it determines an entire economic strategy. The mineralogical properties of soils have been studied in the world for more than 100 years by various characterization techniques, X-ray diffraction being the most prominent. The main difficulty in most of employed techniques is the dominance of the majority phases in the response or the measured signal from the sample that becloud minority phases preventing their identification. The application of methods of phases'separation would provide the possibility to discern minority phases in soils. This work presents a phase separation method that employs a combination of two phenomena based on principles of fluid dynamics: flotation and sedimentation. Different characterization methods were used to analyse the produced soil samples. The methodology employed for separation of phases allowed the complete separation of clay phase from heavier mineral phases. This result makes it possible to discern minority mineral phases of soils that are difficult to detect. A more accurate determination of the mineralogical composition of a soil becomes feasible. |
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Palavras-Chave: |
Caracterização do solo; Separação de fases. |
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Thesagro: |
Mineralogia. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/131256/1/2015-052.pdf
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Marc: |
LEADER 01870nam a2200193 a 4500
001 2026658
005 2021-11-03
008 2015 bl uuuu u00u1 u #d
100 1 $aFRANCHINI, C. A.
245 $aPhase separation methodology for physicochemical studies of soils.$h[electronic resource]
260 $aJournal of Physics: Conference Series, v. 575, 2015. Edition of the Proceedings of VII Brazilian Congress on Metrology, Ouro Preto, Nov. 2013. Ref. 012037.$c2013
520 $aA detailed study of a soil in every country is of a paramount importance, because it determines an entire economic strategy. The mineralogical properties of soils have been studied in the world for more than 100 years by various characterization techniques, X-ray diffraction being the most prominent. The main difficulty in most of employed techniques is the dominance of the majority phases in the response or the measured signal from the sample that becloud minority phases preventing their identification. The application of methods of phases'separation would provide the possibility to discern minority phases in soils. This work presents a phase separation method that employs a combination of two phenomena based on principles of fluid dynamics: flotation and sedimentation. Different characterization methods were used to analyse the produced soil samples. The methodology employed for separation of phases allowed the complete separation of clay phase from heavier mineral phases. This result makes it possible to discern minority mineral phases of soils that are difficult to detect. A more accurate determination of the mineralogical composition of a soil becomes feasible.
650 $aMineralogia
653 $aCaracterização do solo
653 $aSeparação de fases
700 1 $aOLIVEIRA, T. L.
700 1 $aTEIXEIRA, W. G.
700 1 $aKUZNETSOV, A.
700 1 $aACHETE, C. A.
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