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Biblioteca(s): |
Embrapa Hortaliças. |
Data corrente: |
11/11/2004 |
Data da última atualização: |
11/11/2004 |
Autoria: |
GRILLI, G. V. G.; BRAZ, L. R.; LEMOS, E. G. M. |
Título: |
Uso dos marcadores moleculares FAFLP na identificação de QTL´S para tolerância ao calor na característica de fixação de frutos em tomateiro. |
Ano de publicação: |
2004 |
Fonte/Imprenta: |
Horticultura Brasileira, Brasília, v. 22, n. 2, jul. 2004. Suplemento 2. |
Idioma: |
Português |
Notas: |
Trabalho apresentado no 44º Congresso Brasileiro de Olericultura, 2004. Publicado também como resumo em: Horticultura Brasileira, Brasília, v. 22, n. 2, p. 391, jul. 2004. Suplemento 1. |
Palavras-Chave: |
Estresse térmico; Mapa de ligação; Tolerância. |
Thesagro: |
Calor; Lycopersicon Esculentum; Marcador Molecular; Tomate. |
Categoria do assunto: |
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Marc: |
LEADER 00911naa a2200229 a 4500 001 1777060 005 2004-11-11 008 2004 bl uuuu u00u1 u #d 100 1 $aGRILLI, G. V. G. 245 $aUso dos marcadores moleculares FAFLP na identificação de QTL´S para tolerância ao calor na característica de fixação de frutos em tomateiro. 260 $c2004 500 $aTrabalho apresentado no 44º Congresso Brasileiro de Olericultura, 2004. Publicado também como resumo em: Horticultura Brasileira, Brasília, v. 22, n. 2, p. 391, jul. 2004. Suplemento 1. 650 $aCalor 650 $aLycopersicon Esculentum 650 $aMarcador Molecular 650 $aTomate 653 $aEstresse térmico 653 $aMapa de ligação 653 $aTolerância 700 1 $aBRAZ, L. R. 700 1 $aLEMOS, E. G. M. 773 $tHorticultura Brasileira, Brasília$gv. 22, n. 2, jul. 2004. Suplemento 2.
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Registro Completo
Biblioteca(s): |
Embrapa Soja. |
Data corrente: |
05/05/2014 |
Data da última atualização: |
05/08/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
LIU, J.; BRAUN, E.; QIU, W.; SHI, Y. F.; MARCELINO-GUIMARÃES, F. C.; NAVARRE, D. R.; HILL, J. H.; WHITHAM, S. A. |
Afiliação: |
JIAN-ZHONG LIU, Zhejiang Normal University; EDWARD BRAUN, Iowa State University; WEN-LI QIU, Iowa State University; YA-FEI SHI, Zhejiang Normal University; FRANCISMAR CORRÊA MARCELINO-GUIMARÃES, CNPSO; DUROY NAVARRE, Washington State University; JOHN H. HILL, Iowa State University; STEVEN A. WHITHAM, Iowa State University. |
Título: |
Positive and negative roles for soybean MPK6 in regulating defense responses. |
Ano de publicação: |
2014 |
Fonte/Imprenta: |
Molecular Plant-Microbe Interactions, p. 1-36, Apr. 2014. |
ISSN: |
0894-0282 |
DOI: |
http://dx.doi.org/10.1094/MPMI-11-13-0350-R |
Idioma: |
Inglês |
Conteúdo: |
It has been well established that MPK6 is a positive regulator of defense responses in model plants such as Arabidopsis and tobacco. However, the functional importance of soybean MPK6 in disease resistance has not been investigated. Here, we showed that silencing of GmMPK6 in soybean using virus-induced gene silencing mediated by Bean pod mottle virus (BPMV) caused stunted growth and spontaneous cell death on the leaves, a typical phenotype of activated defense responses. Consistent with this phenotype, expression of pathogenesis-related (PR) genes and the conjugated form of salicylic acid were significantly increased in GmMPK6-silenced plants. As expected, GmMPK6-silenced plants were more resistant to downy mildew and Soybean mosaic virus compared with vector control plants, indicating a negative role of GmMPK6 in disease resistance. Interestingly, over-expression of GmMPK6, either transiently in N. benthamiana or stably in Arabidopsis, resulted in HR-like cell death. The HR-like cell death was accompanied by increased PR gene expression, suggesting that GmMPK6, like its counterpart in other plant species, also plays a positive role in cell death induction and defense response. Using bimolecular fluorescence complementation analysis, we determined that GmMKK4 might function upstream of GmMPK6 and GmMKK4 could interact with GmMPK6 independent of its phosphorylation status. Taken together, our results indicate that GmMPK6 functions as both repressor and activator in defense responses of soybean. MenosIt has been well established that MPK6 is a positive regulator of defense responses in model plants such as Arabidopsis and tobacco. However, the functional importance of soybean MPK6 in disease resistance has not been investigated. Here, we showed that silencing of GmMPK6 in soybean using virus-induced gene silencing mediated by Bean pod mottle virus (BPMV) caused stunted growth and spontaneous cell death on the leaves, a typical phenotype of activated defense responses. Consistent with this phenotype, expression of pathogenesis-related (PR) genes and the conjugated form of salicylic acid were significantly increased in GmMPK6-silenced plants. As expected, GmMPK6-silenced plants were more resistant to downy mildew and Soybean mosaic virus compared with vector control plants, indicating a negative role of GmMPK6 in disease resistance. Interestingly, over-expression of GmMPK6, either transiently in N. benthamiana or stably in Arabidopsis, resulted in HR-like cell death. The HR-like cell death was accompanied by increased PR gene expression, suggesting that GmMPK6, like its counterpart in other plant species, also plays a positive role in cell death induction and defense response. Using bimolecular fluorescence complementation analysis, we determined that GmMKK4 might function upstream of GmMPK6 and GmMKK4 could interact with GmMPK6 independent of its phosphorylation status. Taken together, our results indicate that GmMPK6 functions as both repressor and activator in defense r... Mostrar Tudo |
Thesagro: |
Soja. |
Categoria do assunto: |
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Marc: |
LEADER 02217naa a2200241 a 4500 001 1985575 005 2017-08-05 008 2014 bl uuuu u00u1 u #d 022 $a0894-0282 024 7 $ahttp://dx.doi.org/10.1094/MPMI-11-13-0350-R$2DOI 100 1 $aLIU, J. 245 $aPositive and negative roles for soybean MPK6 in regulating defense responses.$h[electronic resource] 260 $c2014 520 $aIt has been well established that MPK6 is a positive regulator of defense responses in model plants such as Arabidopsis and tobacco. However, the functional importance of soybean MPK6 in disease resistance has not been investigated. Here, we showed that silencing of GmMPK6 in soybean using virus-induced gene silencing mediated by Bean pod mottle virus (BPMV) caused stunted growth and spontaneous cell death on the leaves, a typical phenotype of activated defense responses. Consistent with this phenotype, expression of pathogenesis-related (PR) genes and the conjugated form of salicylic acid were significantly increased in GmMPK6-silenced plants. As expected, GmMPK6-silenced plants were more resistant to downy mildew and Soybean mosaic virus compared with vector control plants, indicating a negative role of GmMPK6 in disease resistance. Interestingly, over-expression of GmMPK6, either transiently in N. benthamiana or stably in Arabidopsis, resulted in HR-like cell death. The HR-like cell death was accompanied by increased PR gene expression, suggesting that GmMPK6, like its counterpart in other plant species, also plays a positive role in cell death induction and defense response. Using bimolecular fluorescence complementation analysis, we determined that GmMKK4 might function upstream of GmMPK6 and GmMKK4 could interact with GmMPK6 independent of its phosphorylation status. Taken together, our results indicate that GmMPK6 functions as both repressor and activator in defense responses of soybean. 650 $aSoja 700 1 $aBRAUN, E. 700 1 $aQIU, W. 700 1 $aSHI, Y. F. 700 1 $aMARCELINO-GUIMARÃES, F. C. 700 1 $aNAVARRE, D. R. 700 1 $aHILL, J. H. 700 1 $aWHITHAM, S. A. 773 $tMolecular Plant-Microbe Interactions, p. 1-36, Apr. 2014.
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