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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
14/04/2004 |
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Data da última atualização: |
14/04/2004 |
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Autoria: |
GROLL, J; GRAY, V. M; MYCOCK, D. J. |
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Título: |
Some factores effecting maturation of somatic embryos of cassava derived from friable embryogenic callus. |
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Ano de publicação: |
1998 |
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Fonte/Imprenta: |
Revista Brasileira de Mandioca, Cruz das Almas, v.17, p.52, nov., 1998. Suplemento. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: The regeneretion frequency of plantlets from somatic embryos of many species, including cassava is often limited by the success rate of the developmental events precede germination. With respect to cassava friable embryogernic callus (FEC), three critical developmental events that can influence both the number and quality of germinated plantlets occur after material is removed from liquid culture. These are 1) differentiation of embryos from undifferentiated callus, 2) maturation of the embryos and their acquisition dessication tolerance, and 3) subsequent "germination" after the dessication step. Evidently, some of the factores which are included in the media to trigger these events are best for tissue differentiation but are not suitable for induction of dessication tolerance and germination. The presence of ABA and high concentrations of of sucrose reduced the number of embryos that differentiated from FEC of the clone Mcol 22. However, compared with embryos derived from ABA free and low sucrose concentration media, more of these embryos were capable of surviving dessication and developing into viable plantlets. Other media factors, such as salt concentration with activated charcoal appeared to have a possitive effect on both differentiation and subsequent germination. For each of the above three developmental events, separete culture manipulative stages are required to assure a compromise between the quantity and quality (regenerative ability) of embryos produced. MenosAbstract: The regeneretion frequency of plantlets from somatic embryos of many species, including cassava is often limited by the success rate of the developmental events precede germination. With respect to cassava friable embryogernic callus (FEC), three critical developmental events that can influence both the number and quality of germinated plantlets occur after material is removed from liquid culture. These are 1) differentiation of embryos from undifferentiated callus, 2) maturation of the embryos and their acquisition dessication tolerance, and 3) subsequent "germination" after the dessication step. Evidently, some of the factores which are included in the media to trigger these events are best for tissue differentiation but are not suitable for induction of dessication tolerance and germination. The presence of ABA and high concentrations of of sucrose reduced the number of embryos that differentiated from FEC of the clone Mcol 22. However, compared with embryos derived from ABA free and low sucrose concentration media, more of these embryos were capable of surviving dessication and developing into viable plantlets. Other media factors, such as salt concentration with activated charcoal appeared to have a possitive effect on both differentiation and subsequent germination. For each of the above three developmental events, separete culture manipulative stages are required to assure a compromise between the quantity and quality (regenerative ability) of embryos pr... Mostrar Tudo |
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Marc: |
LEADER 01970naa a2200145 a 4500
001 1651880
005 2004-04-14
008 1998 bl uuuu u00u1 u #d
100 1 $aGROLL, J
245 $aSome factores effecting maturation of somatic embryos of cassava derived from friable embryogenic callus.
260 $c1998
520 $aAbstract: The regeneretion frequency of plantlets from somatic embryos of many species, including cassava is often limited by the success rate of the developmental events precede germination. With respect to cassava friable embryogernic callus (FEC), three critical developmental events that can influence both the number and quality of germinated plantlets occur after material is removed from liquid culture. These are 1) differentiation of embryos from undifferentiated callus, 2) maturation of the embryos and their acquisition dessication tolerance, and 3) subsequent "germination" after the dessication step. Evidently, some of the factores which are included in the media to trigger these events are best for tissue differentiation but are not suitable for induction of dessication tolerance and germination. The presence of ABA and high concentrations of of sucrose reduced the number of embryos that differentiated from FEC of the clone Mcol 22. However, compared with embryos derived from ABA free and low sucrose concentration media, more of these embryos were capable of surviving dessication and developing into viable plantlets. Other media factors, such as salt concentration with activated charcoal appeared to have a possitive effect on both differentiation and subsequent germination. For each of the above three developmental events, separete culture manipulative stages are required to assure a compromise between the quantity and quality (regenerative ability) of embryos produced.
700 1 $aGRAY, V. M
700 1 $aMYCOCK, D. J.
773 $tRevista Brasileira de Mandioca, Cruz das Almas$gv.17, p.52, nov., 1998. Suplemento.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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