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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
09/05/2000 |
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Data da última atualização: |
09/05/2000 |
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Autoria: |
LAMINSKI, S.; GRAY, V. M.; ROBINSON, E. R.; BLATCH, G. L.; MYCOCK, D. J.; GROLL, J. |
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Afiliação: |
Departament of Botany University of the Witwatersrand, South Africa. |
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Título: |
The green fluorescent protein as a reporter of transformation in cassava (Manihot esculenta Crantz) |
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Ano de publicação: |
2000 |
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Fonte/Imprenta: |
Brasilia: Embrapa Recursos Genetico e Biotecnologia/CBN, 2000 |
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Páginas: |
p.222-227 |
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ISBN: |
85-87697-05-6 |
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Idioma: |
Inglês |
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Notas: |
In: INTERNATIONAL SCIENTIFIC MEETING ON CASSAVA BIOTECHNOLOGY, 1998. Salvador-Bahia. Proceedings... Brasilia, DF: Embrapa Recursos Geneticose Biotecnologia/CBN, 2000. 625p. Editado por Luiz J. C. B. Carvalho, Ann Marie Thro, Alberto Duarte Vilarinhos. |
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Conteúdo: |
The objective of this investigation was to assess the use of a mutant form of the green fluorescence protein as a reporter gene in cassava. Sterile leaf explants were plated onto the primary somatic embryogenesis induction medium. After 20 days embryogenic callus was plated onto the secondary embryogenesis proliferation medium. Agrobacterium tumefaciens strain LBA4404 containing binary plasmid XC-1 blue encoding the green fluorescence protein, variant mgfp5, was used to transform secondary somatic embryogenic cells. Callus and embryos were examined after 7, 14 and 28 days following transformation for GFP activity using the confocal microscope (Zeiss inverted microscope LSM410). In the transgenic cassava embryo GFP expression was found predominantly in the pllasmalemma and nuclear membranes. |
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Palavras-Chave: |
Cultivares; Mandioca transgenica; Transgenic cassava. |
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Thesagro: |
Mandioca. |
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Thesaurus Nal: |
cassava; cultivars. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01828naa a2200289 a 4500
001 1645824
005 2000-05-09
008 2000 bl uuuu u00u1 u #d
020 $a85-87697-05-6
100 1 $aLAMINSKI, S.
245 $aThe green fluorescent protein as a reporter of transformation in cassava (Manihot esculenta Crantz)
260 $c2000
300 $ap.222-227
500 $aIn: INTERNATIONAL SCIENTIFIC MEETING ON CASSAVA BIOTECHNOLOGY, 1998. Salvador-Bahia. Proceedings... Brasilia, DF: Embrapa Recursos Geneticose Biotecnologia/CBN, 2000. 625p. Editado por Luiz J. C. B. Carvalho, Ann Marie Thro, Alberto Duarte Vilarinhos.
520 $aThe objective of this investigation was to assess the use of a mutant form of the green fluorescence protein as a reporter gene in cassava. Sterile leaf explants were plated onto the primary somatic embryogenesis induction medium. After 20 days embryogenic callus was plated onto the secondary embryogenesis proliferation medium. Agrobacterium tumefaciens strain LBA4404 containing binary plasmid XC-1 blue encoding the green fluorescence protein, variant mgfp5, was used to transform secondary somatic embryogenic cells. Callus and embryos were examined after 7, 14 and 28 days following transformation for GFP activity using the confocal microscope (Zeiss inverted microscope LSM410). In the transgenic cassava embryo GFP expression was found predominantly in the pllasmalemma and nuclear membranes.
650 $acassava
650 $acultivars
650 $aMandioca
653 $aCultivares
653 $aMandioca transgenica
653 $aTransgenic cassava
700 1 $aGRAY, V. M.
700 1 $aROBINSON, E. R.
700 1 $aBLATCH, G. L.
700 1 $aMYCOCK, D. J.
700 1 $aGROLL, J.
773 $tBrasilia: Embrapa Recursos Genetico e Biotecnologia/CBN, 2000
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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