Data/hora: 23/06/2024 21:07:11

Biblioteca(s): | Embrapa Mandioca e Fruticultura.
Data corrente: | 14/04/2004
Data da última atualização: | 14/04/2004
Autoria: | GROLL, J; GRAY, V. M; MYCOCK, D. J.
Título: | Some factores effecting maturation of somatic embryos of cassava derived from friable embryogenic callus.
Ano de publicação: | 1998
Fonte/Imprenta: | Revista Brasileira de Mandioca, Cruz das Almas, v.17, p.52, nov., 1998. Suplemento.
Idioma: | Inglês
Conteúdo: | Abstract: The regeneretion frequency of plantlets from somatic embryos of many species, including cassava is often limited by the success rate of the developmental  events precede germination. With respect to cassava friable  embryogernic callus (FEC), three critical developmental events that can influence both the number and quality of germinated plantlets occur after material is removed from liquid culture. These are 1) differentiation of embryos from undifferentiated callus, 2) maturation of the embryos and their acquisition dessication tolerance, and 3) subsequent "germination" after the dessication step. Evidently, some of the factores which are included in the media to trigger these events are best for tissue differentiation but are not suitable for induction of dessication tolerance and germination. The presence of ABA and high concentrations of of sucrose reduced the number of embryos that differentiated from FEC of the clone Mcol 22. However, compared with embryos derived from ABA free and low sucrose  concentration media, more of these embryos were capable of surviving dessication and developing into viable plantlets. Other media factors, such as salt concentration with activated charcoal appeared to have a possitive effect on both differentiation and subsequent germination. For each of the above three developmental  events, separete culture manipulative stages are required to assure a compromise between the quantity and quality (regenerative ability) of embryos produced.
Categoria do assunto: | --
Registro original: | Embrapa Mandioca e Fruticultura (CNPMF)
Exemplares: | CNPMF - (20943-1) - ADD - AP - -- - 633.68205 -  -  - DIS