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Registro Completo |
Biblioteca(s): |
Embrapa Cerrados. |
Data corrente: |
10/03/1999 |
Data da última atualização: |
10/03/1999 |
Autoria: |
COLLEVATTI, R. G.; GRATTAPLAGLIA, D.; HAY, J. D. |
Título: |
Effects of habitat fragmentation on genetic variability and conservation of the Brazilian "cerrado" tree species, Caryocar brasiliense Camb (Caryocaraceae): preliminary results. |
Ano de publicação: |
1997 |
Fonte/Imprenta: |
In: TROPICAL diversity: origins, maintenance and conservation: program and abstracts. San Jose, Costa Rica: Association for Tropical Biology/Organization for Tropical Studies, 1997. |
Páginas: |
p.60-61. |
Idioma: |
Português |
Notas: |
Symposium and Annual Meeting. |
Palavras-Chave: |
Conservacao de recursos; Resource conservation. |
Thesagro: |
Caryocar Brasiliense; Cerrado; Habitat; Pequi; Variação Genética. |
Thesaurus Nal: |
genetic variation; habitats. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00982naa a2200265 a 4500 001 1544795 005 1999-03-10 008 1997 bl uuuu u00u1 u #d 100 1 $aCOLLEVATTI, R. G. 245 $aEffects of habitat fragmentation on genetic variability and conservation of the Brazilian "cerrado" tree species, Caryocar brasiliense Camb (Caryocaraceae)$bpreliminary results. 260 $c1997 300 $ap.60-61. 500 $aSymposium and Annual Meeting. 650 $agenetic variation 650 $ahabitats 650 $aCaryocar Brasiliense 650 $aCerrado 650 $aHabitat 650 $aPequi 650 $aVariação Genética 653 $aConservacao de recursos 653 $aResource conservation 700 1 $aGRATTAPLAGLIA, D. 700 1 $aHAY, J. D. 773 $tIn: TROPICAL diversity: origins, maintenance and conservation: program and abstracts. San Jose, Costa Rica: Association for Tropical Biology/Organization for Tropical Studies, 1997.
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Registro original: |
Embrapa Cerrados (CPAC) |
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Registro Completo
Biblioteca(s): |
Embrapa Agroindústria Tropical; Embrapa Alimentos e Territórios. |
Data corrente: |
17/11/2023 |
Data da última atualização: |
23/01/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
SANTOS, A. T. L. dos; CARNEIRO, J. N. P.; CRUZ, R. P. da; SALES, D. L.; PINHEIRO, J. C. A.; FREITAS, M. A. de; KERNTOPF, M. R.; DELMONDES, G. de A.; RIBEIRO, P. R. V.; BRITO, E. S. de; BATISTA, F. L. A.; MAGALHÃES, F. E. A.; PITA NETO, I. C.; BRAGA, M. F. B. M.; KOWALSKI, R.; KOWALSKA, G.; SZOPA, A.; BAJ, T.; COUTINHO, H. D. M. |
Afiliação: |
ANTONIA THASSYA LUCAS DOS SANTOS, UNIVERSIDADE REGIONAL DO CARIRI; JOARA NÁLYDA PEREIRA CARNEIRO, UNIVERSIDADE REGIONAL DO CARIRI; RAFAEL PEREIRA DA CRUZ, UNIVERSIDADE REGIONAL DO CARIRI; DÉBORA LIMA SALES, UNIVERSIDADE REGIONAL DO CARIRI; JACQUELINE COSMO ANDRADE-PINHEIRO, UNIVERSIDADE REGIONAL DO CARIRI; MARIA AUDILENE DE FREITAS, UNIVERSIDADE FEDERAL DE PERNAMBUCO; MARTA REGINA KERNTOPF, UNIVERSIDADE REGIONAL DO CARIRI; GYLLYANDESON DE ARAÚJO DELMONDES, UNIVERSIDADE REGIONAL DO CARIRI; PAULO RICELI VASCONCELOS RIBEIRO, CNPAT; EDY SOUSA DE BRITO, CNAT; FRANCISCO LUCAS ALVES BATISTA, UNIVERSIDADE REGIONAL DO CARIRI; FRANCISCO ERNANI ALVES MAGALHÃES, UNIVERSIDADE ESTADUAL DO CEARÁ; IVO CAVALCANTE PITA NETO, FACULDADE CECAPE; MARIA FLAVIANA BEZERRA MORAIS-BRAGA, UNIVERSIDADE REGIONAL DO CARIRI; RADOSŁAW KOWALSKI, UNIWERSYTET PRZYRODNICZNY W LUBLINIE; GRAZYNA KOWALSKA, UNIWERSYTET PRZYRODNICZNY W LUBLINIE; ALEKSANDRA SZOPA, UNIWERSYTET MEDYCZNY W LUBLINIE; TOMASZ BAJ, UNIWERSYTET MEDYCZNY W LUBLINIE; HENRIQUE DOUGLAS MELO COUTINHO, UNIVERSIDADE REGIONAL DO CARIRI. |
Título: |
UPLC-MS-ESI-QTOF analysis and antifungal activity of aqueous extracts of Spondias tuberosa. |
Ano de publicação: |
2023 |
Fonte/Imprenta: |
Molecules, Basel, v. 28, n. 1, art. 305, Jan. 2023. |
Páginas: |
14 p. |
DOI: |
https://doi.org/10.3390/molecules28010305 |
Idioma: |
Inglês |
Conteúdo: |
This study aimed to identify the chemical composition of the Spondias tuberosa aqueous leaf and root extracts (EALST and EARST) and to evaluate their effect, comparatively, against opportunistic pathogenic fungi. Ultra-Performance Liquid Chromatography Coupled to a Quadrupole/Time of Flight System (UPLC-MS-ESI-QTOF) was employed for chemical analysis. Candida albicans and C. tropicalis standard strains and clinical isolates were used (CA INCQS 40006, CT INCQS 40042, CA URM 5974, and CT URM 4262). The 50% Inhibitory Concentration for the fungal population (IC50) was determined for both the intrinsic action of the extracts and the extract/fluconazole (FCZ) associations. The determination of the Minimum Fungicidal Concentration (MFC) and the verification of effects over fungal morphological transitions were performed by subculture in Petri dishes and humid chambers, respectively, both based on micro-dilution. UPLC-MS-ESI-QTOF analysis revealed the presence of phenolic and flavonoid compounds. The association of the extracts with fluconazole, resulted in IC50 values from 2.62 µg/mL to 308.96 µg/mL. The MFC of the extracts was ?16,384 µg/mL for all tested strains, while fluconazole obtained an MFC of 8192 µg/mL against C. albicans strains. A reduction in MFC against CA URM 5974 (EALST: 2048 µg/mL and EARST: 1024 µg/mL) occurred in the extract/fluconazole association. |
Palavras-Chave: |
Popular medicine. |
Thesagro: |
Cromatografia; Spondias Tuberosa; Umbu. |
Thesaurus NAL: |
Electrospray ionization mass spectrometry; Flavonoids; Fluconazole; Mass spectrometry; Ultra-performance liquid chromatography. |
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal X Pesquisa, Tecnologia e Engenharia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1159154/1/uplc-ms-esi-qtof-analysis-and-antifungal-activity-of-aqueous-extracts-of-spondias-tuberosa-2023.pdf
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Marc: |
LEADER 02772naa a2200469 a 4500 001 2159154 005 2024-01-23 008 2023 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.3390/molecules28010305$2DOI 100 1 $aSANTOS, A. T. L. dos 245 $aUPLC-MS-ESI-QTOF analysis and antifungal activity of aqueous extracts of Spondias tuberosa.$h[electronic resource] 260 $c2023 300 $a14 p. 520 $aThis study aimed to identify the chemical composition of the Spondias tuberosa aqueous leaf and root extracts (EALST and EARST) and to evaluate their effect, comparatively, against opportunistic pathogenic fungi. Ultra-Performance Liquid Chromatography Coupled to a Quadrupole/Time of Flight System (UPLC-MS-ESI-QTOF) was employed for chemical analysis. Candida albicans and C. tropicalis standard strains and clinical isolates were used (CA INCQS 40006, CT INCQS 40042, CA URM 5974, and CT URM 4262). The 50% Inhibitory Concentration for the fungal population (IC50) was determined for both the intrinsic action of the extracts and the extract/fluconazole (FCZ) associations. The determination of the Minimum Fungicidal Concentration (MFC) and the verification of effects over fungal morphological transitions were performed by subculture in Petri dishes and humid chambers, respectively, both based on micro-dilution. UPLC-MS-ESI-QTOF analysis revealed the presence of phenolic and flavonoid compounds. The association of the extracts with fluconazole, resulted in IC50 values from 2.62 µg/mL to 308.96 µg/mL. The MFC of the extracts was ?16,384 µg/mL for all tested strains, while fluconazole obtained an MFC of 8192 µg/mL against C. albicans strains. A reduction in MFC against CA URM 5974 (EALST: 2048 µg/mL and EARST: 1024 µg/mL) occurred in the extract/fluconazole association. 650 $aElectrospray ionization mass spectrometry 650 $aFlavonoids 650 $aFluconazole 650 $aMass spectrometry 650 $aUltra-performance liquid chromatography 650 $aCromatografia 650 $aSpondias Tuberosa 650 $aUmbu 653 $aPopular medicine 700 1 $aCARNEIRO, J. N. P. 700 1 $aCRUZ, R. P. da 700 1 $aSALES, D. L. 700 1 $aPINHEIRO, J. C. A. 700 1 $aFREITAS, M. A. de 700 1 $aKERNTOPF, M. R. 700 1 $aDELMONDES, G. de A. 700 1 $aRIBEIRO, P. R. V. 700 1 $aBRITO, E. S. de 700 1 $aBATISTA, F. L. A. 700 1 $aMAGALHÃES, F. E. A. 700 1 $aPITA NETO, I. C. 700 1 $aBRAGA, M. F. B. M. 700 1 $aKOWALSKI, R. 700 1 $aKOWALSKA, G. 700 1 $aSZOPA, A. 700 1 $aBAJ, T. 700 1 $aCOUTINHO, H. D. M. 773 $tMolecules, Basel$gv. 28, n. 1, art. 305, Jan. 2023.
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