Data/hora: 18/06/2024 19:45:35

Biblioteca(s): | Embrapa Unidades Centrais.
Data corrente: | 15/07/2009
Data da última atualização: | 07/06/2018
Autoria: | BONA, C. M. de; GOULD, J. H.; MILLER JUNIOR, J. C.; STELLY, D.; LOUZADA, E. S.
Afiliação: | Claudine Maria de Bona, Instituto Agronômico do Paraná; Jean Howe Gould, Texas A&M University - TAMU/Department of Ecosystem Science and Management; J. Creighton Miller Jr, TAMU/Department of Horticultural Sciences, College Station Texas; David Stelly, TAMU/Department of Soil & Crop Sciences, College Station; Eliezer Silva Louzada, Texas A&M University?Kingsville.
Título: | Citrus asymmetric somatic hybrids produced via fusion of gamma-irradiated and iodoacetamide-treated protoplasts.
Ano de publicação: | 2009
Fonte/Imprenta: | Pesquisa Agropecuária Brasileira, Brasília, DF, v. 44, n. 5, p. 454-462, maio 2009
Idioma: | Inglês
Notas: | Título em português: Híbridos somáticos assimétricos de citros produzidos pela fusão de protoplastos irradiados e tratados com iodoacetamida.
Conteúdo: | The objective of this study was to produce citrus somatic asymmetric hybrids by fusing gamma-irradiated protoplasts with iodoacetamide-treated protoplasts. Protoplasts were isolated from embryogenic suspension cells of grapefruit (Citrus paradisi Macfad.) cultivars Ruby Red and Flame, sweet oranges (C. sinensis Osbeck) 'Itaboraí', 'Natal', Valencia', and 'Succari', from 'Satsuma' (C. unshiu Marcow.) and 'Changsha' mandarin (C. reticulata Blanco) and 'Murcott' tangor (C. reticulata x C. sinensis). Donor protoplasts were exposed to gamma rays and receptor protoplasts were treated with 3 mmol L-1 iodoacetamide (IOA), and then they were fused for asymmetric hybridization. Asymmetric embryos were germinated, and the resulting shoots were either grafted onto sour orange, rough lemon or 'Swingle' (C. paradisi x Poncirus trifoliata) x 'Sunki' mandarin rootstock seedlings, or rooted after dipping their bases in indol-butyric acid (IBA) solution. The products were later acclimatized to greenhouse conditions. Ploidy was analyzed by flow cytometry, and hybridity was confirmed by amplified fragment length polymorphism (AFLP) analysis of plantlet DNA samples. The best treatment was the donor-recipient fusion combination of 80 Gy-irradiated 'Ruby Red' protoplasts with 20 min IOA-treated 'Succari' protoplasts. Tetraploid and aneuploid plants were produced. Rooting recalcitrance was solved by dipping shoots' stems in 3,000 mg L-1 IBA solution for 10 min.
Palavras-Chave: | fusão de protoplasto; hibridação somática.
Thesagro: | Fruta Cítrica; Laranja Doce; Pomelo; Raio Gama.
Thesaurus Nal: | Gamma radiation; Grapefruits; Oranges; Protoplast fusion; Somatic hybridization.
Categoria do assunto: | --
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/38067/1/44n05a04.pdf
Registro original: | Embrapa Unidades Centrais (AI-SEDE)
Exemplares: | AI-SEDE - (45905-1) - UPE - AP - PP - 630.72081 - P474 -  - DIS