Data/hora: 21/06/2024 18:06:44

Biblioteca(s): 	Embrapa Mandioca e Fruticultura.	
Data corrente: 	18/08/1999	
Data da última atualização: 	16/05/2023	
Autoria: 	FITCH, M. M. M.; MANSHARDT, R. M.; GONSALVES, D.; SLIGHTOM, J. L.; SANFORD, J. C.	
Afiliação: 	University of Hawaii; University Geneva; The Upjohn Company, Kalamazoo, MI 49001, USA.	
Título: 	Stable transformation of papaya via microprojectile bombardment.	
Ano de publicação: 	1990	
Fonte/Imprenta: 	Plant Cell Reports, v.9, p.189-194, 1990.	
Idioma: 	Inglês	
Conteúdo: 	Stable transformation of papaya (carica papaya L.) has been achieved following DNA delivery via high velocity microprojectiles. Three types of embryogenic tissues, including immature zygotic embryos, freshly explanted hypocotyl sections, and somatic embryos derived from both, were bombarded with tungsten particles carrying chimeric NPTII and GUS genes. All tissue types were cultured prior to and following bombardment on half-strength MS medium supplemented with 10mg 1-1 2,4-D, 400mg 1-1 glutamine, and 6% sucrose. Upon transfer to 2,4-D-free medium containing 150mg 1-1 kanamycin sulfate, ten putative transgenic isolates produced somatic embryos and five regenerated leafy shoots.	
Thesagro: 	Mamão.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Mandioca e Fruticultura (CNPMF)	
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