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Registro Completo |
Biblioteca(s): |
Embrapa Acre. |
Data corrente: |
11/06/2014 |
Data da última atualização: |
06/07/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SILVA, C. C.; MANTELLO, C. C.; CAMPOS, T. de; SOUZA, L. M.; GONÇALVES, P. S.; SOUZA, A. P. |
Afiliação: |
Carla C. Silva, Universidade Estadual de Campinas (Unicamp); Camila C. Mantello, Universidade Estadual de Campinas (Unicamp); TATIANA DE CAMPOS, CPAF-AC; Livia M. Souza, Universidade Estadual de Campinas (Unicamp); Paulo S. Gonçalves, Instituto Agronômico de Campinas (IAC); Anete P. Souza, Universidade Estadual de Campinas (Unicamp). |
Título: |
Leaf-, panel- and latex-expressed sequenced tags from the rubber tree (Hevea brasiliensis) under cold-stressed and suboptimal growing conditions: the development of gene-targeted Functional markers for stress response. |
Ano de publicação: |
2014 |
Fonte/Imprenta: |
Molecular Breeding, Berlim, v. 34, n. 3, p. 1035-1053, Oct. 2014. |
ISSN: |
1380-3743 (impresso) / 1572-9788 (on-line) |
DOI: |
10.1007/s11032-014-0095-2 |
Idioma: |
Inglês |
Conteúdo: |
Hevea brasiliensis is a native species of the Amazon Basin of South America and the primary source of natural rubber worldwide. Due to the occurrence of South American Leaf Blight disease in this area, rubber plantations have been extended to suboptimal regions. Rubber tree breeding is time-consuming and expensive, but molecular markers can serve as a tool for early valuation, thus reducing time and costs. In this work, we constructed six different cDNA libraries with the aim of developing gene-targeted molecular markers for the rubber tree. A total of 8,263 reads were assembled, generating 5,025 unigenes that were analyzed; 912 expressed sequence tags (ESTs) represented new transcripts, and two sequences were highly up-regulated by cold stress. These unigenes were scanned for microsatellite (SSR) regions and single nucleotide polymorphisms (SNPs). In total, 169 novel EST-SSR markers were developed; 138 loci were polymorphic in the rubber tree, and 98 % presented transferability to six other Hevea species. Locus duplication was observed in H. brasil-iensis and other species. Additionally, 43 SNP markers in 13 sequences that showed similarity to proteins involved in stress response, latex biosynthesis and developmental processes were characterized. cDNA libraries are a rich source of SSR and SNP markers and enable the identification of new transcripts. The new markers developed here will be a valuable resource for linkage mapping, QTL identification and other studies in the rubber tree and can also be used to evaluate the genetic variability of other Hevea species, which are valuable assets in rubber tree breeding. MenosHevea brasiliensis is a native species of the Amazon Basin of South America and the primary source of natural rubber worldwide. Due to the occurrence of South American Leaf Blight disease in this area, rubber plantations have been extended to suboptimal regions. Rubber tree breeding is time-consuming and expensive, but molecular markers can serve as a tool for early valuation, thus reducing time and costs. In this work, we constructed six different cDNA libraries with the aim of developing gene-targeted molecular markers for the rubber tree. A total of 8,263 reads were assembled, generating 5,025 unigenes that were analyzed; 912 expressed sequence tags (ESTs) represented new transcripts, and two sequences were highly up-regulated by cold stress. These unigenes were scanned for microsatellite (SSR) regions and single nucleotide polymorphisms (SNPs). In total, 169 novel EST-SSR markers were developed; 138 loci were polymorphic in the rubber tree, and 98 % presented transferability to six other Hevea species. Locus duplication was observed in H. brasil-iensis and other species. Additionally, 43 SNP markers in 13 sequences that showed similarity to proteins involved in stress response, latex biosynthesis and developmental processes were characterized. cDNA libraries are a rich source of SSR and SNP markers and enable the identification of new transcripts. The new markers developed here will be a valuable resource for linkage mapping, QTL identification and other studies in the r... Mostrar Tudo |
Palavras-Chave: |
Biblioteca cDNA; CDNA library; Fitomejoramiento; Marcador microssatélite; Marcador SNP; Marcadores genéticos; Repeticiones de microsatélite; Rubber tree. |
Thesagro: |
Caucho; DNA; Hevea brasiliensis; Marcador genético; Melhoramento genético vegetal; Seringueira. |
Thesaurus Nal: |
Genetic markers; Microsatellite repeats; Plant breeding. |
Categoria do assunto: |
G Melhoramento Genético |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/160345/1/25073.pdf
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Marc: |
LEADER 02997naa a2200409 a 4500 001 1988256 005 2021-07-06 008 2014 bl uuuu u00u1 u #d 022 $a1380-3743 (impresso) / 1572-9788 (on-line) 024 7 $a10.1007/s11032-014-0095-2$2DOI 100 1 $aSILVA, C. C. 245 $aLeaf-, panel- and latex-expressed sequenced tags from the rubber tree (Hevea brasiliensis) under cold-stressed and suboptimal growing conditions$bthe development of gene-targeted Functional markers for stress response.$h[electronic resource] 260 $c2014 520 $aHevea brasiliensis is a native species of the Amazon Basin of South America and the primary source of natural rubber worldwide. Due to the occurrence of South American Leaf Blight disease in this area, rubber plantations have been extended to suboptimal regions. Rubber tree breeding is time-consuming and expensive, but molecular markers can serve as a tool for early valuation, thus reducing time and costs. In this work, we constructed six different cDNA libraries with the aim of developing gene-targeted molecular markers for the rubber tree. A total of 8,263 reads were assembled, generating 5,025 unigenes that were analyzed; 912 expressed sequence tags (ESTs) represented new transcripts, and two sequences were highly up-regulated by cold stress. These unigenes were scanned for microsatellite (SSR) regions and single nucleotide polymorphisms (SNPs). In total, 169 novel EST-SSR markers were developed; 138 loci were polymorphic in the rubber tree, and 98 % presented transferability to six other Hevea species. Locus duplication was observed in H. brasil-iensis and other species. Additionally, 43 SNP markers in 13 sequences that showed similarity to proteins involved in stress response, latex biosynthesis and developmental processes were characterized. cDNA libraries are a rich source of SSR and SNP markers and enable the identification of new transcripts. The new markers developed here will be a valuable resource for linkage mapping, QTL identification and other studies in the rubber tree and can also be used to evaluate the genetic variability of other Hevea species, which are valuable assets in rubber tree breeding. 650 $aGenetic markers 650 $aMicrosatellite repeats 650 $aPlant breeding 650 $aCaucho 650 $aDNA 650 $aHevea brasiliensis 650 $aMarcador genético 650 $aMelhoramento genético vegetal 650 $aSeringueira 653 $aBiblioteca cDNA 653 $aCDNA library 653 $aFitomejoramiento 653 $aMarcador microssatélite 653 $aMarcador SNP 653 $aMarcadores genéticos 653 $aRepeticiones de microsatélite 653 $aRubber tree 700 1 $aMANTELLO, C. C. 700 1 $aCAMPOS, T. de 700 1 $aSOUZA, L. M. 700 1 $aGONÇALVES, P. S. 700 1 $aSOUZA, A. P. 773 $tMolecular Breeding, Berlim$gv. 34, n. 3, p. 1035-1053, Oct. 2014.
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Embrapa Acre (CPAF-AC) |
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Registros recuperados : 9 | |
2. | | PEDROSA, M. B.; RIOS, D. M.; NASCIMENTO, L. R. do; SANTOS, C. A. A. dos; OLIVEIRA, J. da S. Avaliação de linhagens avançadas de fibra colorida nos múnicipios de Angical e Wanderley-BA. In: CONGRESSO BRASILEIRO DO ALGODÃO, 8.; COTTON EXPO, 1., 2011, São Paulo. Evolução da cadeia para construção de um setor forte: Anais. Campina Grande, PB: Embrapa Algodão, 2011. p.12801285Biblioteca(s): Embrapa Algodão. |
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4. | | SANTOS, C. A. A. DOS; FERNANDES, V. J.; GUERRA, J. G. M.; CEDDIA, M. B.; AGUIAR-MENEZES, E. de L. Diversidade de Coccinelídeos (Coleoptera) capturados por armadilha adesiva em cultivo agroecológico no município de Seropédica, RJ In: SIMPÓSIO DE FITOSSANIDADE E BIOTECNOLOGIA APLICADA, 5., 2019, Seropédica. Resumos... Seropédica: UFRRJ, 2019.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Agrobiologia. |
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6. | | SANTOS, J. B. dos; AZEVEDO, A. V. de; RIOS, D. M.; SANTOS, C. A. A.; SANTIAGO, A. N.; BELTRÃO, N. E. de M.; ALVES, G. da S. Desempenho vegetativo de cultivares de mamona em regime de sequeiro no município de Angical- BA. In: CONGRESSO BRASILEIRO DE MAMONA, 3., 2008, Salvador. Energia e ricinoquímica: resumos. Salvador: SEAGRI: Embrapa Algodão, 2008. p. 125Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Algodão. |
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7. | | SANTOS, J. B. dos; AZEVEDO, A. V. de; RIOS, D. M.; SANTOS, C. A. A.; SANTIAGO, A. N.; BELTRÃO, N. E. de M.; ALVES, G. da S. Desempenho vegetativo de cultivares de mamona em regime de sequeiro no município de Angical- BA. In: CONGRESSO BRASILEIRO DE MAMONA, 3., 2008, Salvador. Energia e ricinoquímica: anais. Salvador: SEAGRI: Embrapa Algodão, 2008. 1 CD-ROMTipo: Artigo em Anais de Congresso / Nota Técnica |
Biblioteca(s): Embrapa Algodão. |
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8. | | SANTOS, J. B. dos; AZEVEDO, C. A. V. de; RIOS, D. M.; SANTOS, C. A. A.; SANTIAGO, A. N.; BELTRÃO, N. E. de M.; ALVES, G. da S. Desenvolvimento produtivo de cultivares de mamona em regime de sequeiro no município de Angical - BA. In: CONGRESSO BRASILEIRO DE MAMONA, 3., 2008, Salvador. Energia e ricinoquímica: resumos. Salvador: SEAGRI: Embrapa Algodão, 2008. p. 108Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Algodão. |
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9. | | SANTOS, J. B. dos; AZEVEDO, C. A. V. de; RIOS, D. M.; SANTOS, C. A. A.; SANTIAGO, A. N.; BELTRÃO, N. E. de M.; ALVES, G. da S. Desenvolvimento produtivo de cultivares de mamona em regime de sequeiro no município de Angical - BA. In: CONGRESSO BRASILEIRO DE MAMONA, 3., 2008, Salvador. Energia e ricinoquímica: anais. Salvador: SEAGRI: Embrapa Algodão, 2008. 1 CD-ROMTipo: Artigo em Anais de Congresso / Nota Técnica |
Biblioteca(s): Embrapa Algodão. |
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Registros recuperados : 9 | |
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