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Registro Completo |
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
Data corrente: |
17/08/2016 |
Data da última atualização: |
17/08/2016 |
Autoria: |
AMORIM, C. A.; RODRIGUES, A. P. R.; LUCCI, C. M.; FIGUEIREDO, J. R.; GONÇALVES, P. B. D. |
Título: |
Método mecânico para o isolamento de folículos pré-antrais a partir de ovários de ovelhas adultas. |
Ano de publicação: |
1998 |
Fonte/Imprenta: |
Arquivos da Faculdade de Veterinária UFRGS, v. 26, n. 1, p. 214, 1998. Suplemento. |
Idioma: |
Português |
Notas: |
Resumo apresentado na 13a. Reunião Anual da Sociedade Brasileira de Transferência de Embriões, 1998, Atibaia. |
Conteúdo: |
Este estudo mostra, pela primeira vez, o desenvolvimento de um método mecâncio rápido e eficienmte para o isolamento de um grande números de folículos pré-antrais intacto de ovários de ovelhas adultas.
[Mechanical method for the isolation of preantral follicles from adult ovine ovaries]. |
Palavras-Chave: |
Folículos pré-antrais. |
Thesagro: |
Ovário; Ovelha; Ovino; Reprodução animal. |
Thesaurus Nal: |
Ewes; Sheep. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 01159naa a2200265 a 4500 001 2051111 005 2016-08-17 008 1998 bl uuuu u00u1 u #d 100 1 $aAMORIM, C. A. 245 $aMétodo mecânico para o isolamento de folículos pré-antrais a partir de ovários de ovelhas adultas. 260 $c1998 500 $aResumo apresentado na 13a. Reunião Anual da Sociedade Brasileira de Transferência de Embriões, 1998, Atibaia. 520 $aEste estudo mostra, pela primeira vez, o desenvolvimento de um método mecâncio rápido e eficienmte para o isolamento de um grande números de folículos pré-antrais intacto de ovários de ovelhas adultas. [Mechanical method for the isolation of preantral follicles from adult ovine ovaries]. 650 $aEwes 650 $aSheep 650 $aOvário 650 $aOvelha 650 $aOvino 650 $aReprodução animal 653 $aFolículos pré-antrais 700 1 $aRODRIGUES, A. P. R. 700 1 $aLUCCI, C. M. 700 1 $aFIGUEIREDO, J. R. 700 1 $aGONÇALVES, P. B. D. 773 $tArquivos da Faculdade de Veterinária UFRGS$gv. 26, n. 1, p. 214, 1998. Suplemento.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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Registro Completo
Biblioteca(s): |
Embrapa Meio-Norte. |
Data corrente: |
19/09/2022 |
Data da última atualização: |
17/02/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 4 |
Autoria: |
SÁ, G. H.; LIMA, F. C. D.; VIANA, J. P. G.; LOPES, A. C. A.; CARVALHO, L. C. B.; VALENTE, S. E. S.; LIMA, P. S. da C. |
Afiliação: |
GISELE H. SÁ, UNIVERSIDADE FEDERAL DO PIAUÍ; FATIANNE C. D. LIMA, UNIVERSIDADE FEDERAL DO PIAUÍ; JOÃO P. G. VIANA, UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN; ÂNGELA, C. A. LOPES, UNIVERSIDADE FEDERAL DO PIAUÍ; LEONARDO C. B. CARVALHO, UNIVERSIDADE FEDERAL DO PIAUÍ; SÉRGIO E. S. VALENTE, UNIVERSIDADE FEDERAL DO PIAUÍ; PAULO SARMANHO DA COSTA LIMA, CPAMN. |
Título: |
Genetic diversity and structure of an active germplasm collection of Annona squamosa L. |
Ano de publicação: |
2022 |
Fonte/Imprenta: |
Ecological Genetics and Genomics, v. 25, e100143, Sep. 2022. |
ISSN: |
2405-9854 |
DOI: |
10.1016/j.egg.2022.100143 |
Idioma: |
Inglês |
Conteúdo: |
The fruits of Annona squamosa L. are highly appreciated for their sweet taste and their nutritional and medicinal properties. Since the species is threatened by habitat loss, germplasm conservation is of the utmost importance. The present study aimed to characterize genetic variability among 19 accessions of A. squamosa maintained in the Active Germplasm Bank (AGB) of Embrapa Meio-Norte, Teresina, Piauí, Brazil, and to determine whether the geographical distribution of the genotypes influenced population structure. The accessions were derived from populations originally located in orchards situated some 200 km apart in three municipalities in the northeast of Brazil, namely Timon, Bom Jesus and Canto do Buriti. Leaf DNA was extracted and analyzed by inter simple sequence repeat (ISSR)-polymerase chain reaction. Nine ISSR primers generated 127 scorable bands of which 101 (79.52%) were polymorphic. The within-population diversity was high (Canto do Buriti), intermediary (Timon) and low (Bom Jesus). Unweighted pair-group method with arithmetic averages and Bayesian analysis of polymorphic bands separated the genotypes into two main clusters according to genetic distance and ancestry (vegetative- and seed-propagated groups), indicating that the geographical location of the populations did not influence the genetic diversity of the accessions. While ISSR marker-based DNA fingerprinting of the 19 studied accessions revealed a degree of genetic diversity between populations, the variability detected within each population was somewhat higher. This information will contribute to a better understanding of genetic variation and population structure of the accessions of A. squamosa in the AGB of Embrapa Meio-Norte, and provide crucial information for future conservation and genetic improvement programs of the species. MenosThe fruits of Annona squamosa L. are highly appreciated for their sweet taste and their nutritional and medicinal properties. Since the species is threatened by habitat loss, germplasm conservation is of the utmost importance. The present study aimed to characterize genetic variability among 19 accessions of A. squamosa maintained in the Active Germplasm Bank (AGB) of Embrapa Meio-Norte, Teresina, Piauí, Brazil, and to determine whether the geographical distribution of the genotypes influenced population structure. The accessions were derived from populations originally located in orchards situated some 200 km apart in three municipalities in the northeast of Brazil, namely Timon, Bom Jesus and Canto do Buriti. Leaf DNA was extracted and analyzed by inter simple sequence repeat (ISSR)-polymerase chain reaction. Nine ISSR primers generated 127 scorable bands of which 101 (79.52%) were polymorphic. The within-population diversity was high (Canto do Buriti), intermediary (Timon) and low (Bom Jesus). Unweighted pair-group method with arithmetic averages and Bayesian analysis of polymorphic bands separated the genotypes into two main clusters according to genetic distance and ancestry (vegetative- and seed-propagated groups), indicating that the geographical location of the populations did not influence the genetic diversity of the accessions. While ISSR marker-based DNA fingerprinting of the 19 studied accessions revealed a degree of genetic diversity between populations, the va... Mostrar Tudo |
Palavras-Chave: |
ISSR; Pinha. |
Thesagro: |
Conservação; Fruta Tropical; Marcador Molecular; Polimorfismo Genético. |
Thesaurus NAL: |
Conservation plants; Genetic markers; Genetic polymorphism; Tropical and subtropical fruits. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1146583/1/GeneticDiversityStructureActiveGermplasmCollectionAnnonaEGG25.2022.pdf
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Marc: |
LEADER 02832naa a2200337 a 4500 001 2146583 005 2023-02-17 008 2022 bl uuuu u00u1 u #d 022 $a2405-9854 024 7 $a10.1016/j.egg.2022.100143$2DOI 100 1 $aSÁ, G. H. 245 $aGenetic diversity and structure of an active germplasm collection of Annona squamosa L.$h[electronic resource] 260 $c2022 520 $aThe fruits of Annona squamosa L. are highly appreciated for their sweet taste and their nutritional and medicinal properties. Since the species is threatened by habitat loss, germplasm conservation is of the utmost importance. The present study aimed to characterize genetic variability among 19 accessions of A. squamosa maintained in the Active Germplasm Bank (AGB) of Embrapa Meio-Norte, Teresina, Piauí, Brazil, and to determine whether the geographical distribution of the genotypes influenced population structure. The accessions were derived from populations originally located in orchards situated some 200 km apart in three municipalities in the northeast of Brazil, namely Timon, Bom Jesus and Canto do Buriti. Leaf DNA was extracted and analyzed by inter simple sequence repeat (ISSR)-polymerase chain reaction. Nine ISSR primers generated 127 scorable bands of which 101 (79.52%) were polymorphic. The within-population diversity was high (Canto do Buriti), intermediary (Timon) and low (Bom Jesus). Unweighted pair-group method with arithmetic averages and Bayesian analysis of polymorphic bands separated the genotypes into two main clusters according to genetic distance and ancestry (vegetative- and seed-propagated groups), indicating that the geographical location of the populations did not influence the genetic diversity of the accessions. While ISSR marker-based DNA fingerprinting of the 19 studied accessions revealed a degree of genetic diversity between populations, the variability detected within each population was somewhat higher. This information will contribute to a better understanding of genetic variation and population structure of the accessions of A. squamosa in the AGB of Embrapa Meio-Norte, and provide crucial information for future conservation and genetic improvement programs of the species. 650 $aConservation plants 650 $aGenetic markers 650 $aGenetic polymorphism 650 $aTropical and subtropical fruits 650 $aConservação 650 $aFruta Tropical 650 $aMarcador Molecular 650 $aPolimorfismo Genético 653 $aISSR 653 $aPinha 700 1 $aLIMA, F. C. D. 700 1 $aVIANA, J. P. G. 700 1 $aLOPES, A. C. A. 700 1 $aCARVALHO, L. C. B. 700 1 $aVALENTE, S. E. S. 700 1 $aLIMA, P. S. da C. 773 $tEcological Genetics and Genomics$gv. 25, e100143, Sep. 2022.
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