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Registro Completo |
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Biblioteca(s): |
Embrapa Agroenergia; Embrapa Milho e Sorgo. |
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Data corrente: |
19/09/2017 |
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Data da última atualização: |
15/02/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SOUSA, C. A. F. de; PAIVA, D S. de; CASARI, R. A. das C. N.; MOLINARI, H. B. C.; OLIVEIRA, N. G. de; KOBAYASHI, A. K.; MAGALHAES, P. C.; GOMIDE, R. L.; SOUZA JUNIOR, M. T. |
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Afiliação: |
CARLOS ANTONIO FERREIRA DE SOUSA, CNPAE; DAYANE SILVA DE PAIVA; RAPHAEL AUGUSTO DAS CHAGAS NOQUELI CASARI; HUGO BRUNO CORREA MOLINARI, CNPAE; NELSON GERALDO DE OLIVEIRA; ADILSON KENJI KOBAYASHI, CNPAE; PAULO CESAR MAGALHAES, CNPMS; REINALDO LUCIO GOMIDE, CNPMS; MANOEL TEIXEIRA SOUZA JUNIOR, CNPAE. |
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Título: |
A procedure for maize genotypes discrimination to drought by chlorophyll fluorescence imaging rapid light curves. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Plant Methods, v. 13, p. 1-17, 2017. |
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DOI: |
10.1186/s13007-017-0209-z |
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Idioma: |
Inglês |
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Conteúdo: |
Background: Photosynthesis can be roughly separated into biochemical and photochemical processes. Both are affected by drought and can be assessed by non-invasive standard methods. Gas exchange, which mainly assesses the first process, has well-defined protocols. It is considered a standard method for evaluation of plant responses to drought. Under such stress, assessment of photochemical apparatus by chlorophyll fluorescence needs improvement to become faster and reproducible, especially in growing plants under field conditions. For this, we developed a protocol based on chlorophyll fluorescence imaging, using a rapid light curve approach. Results: Almost all parameters obtained by rapid light curves have shown statistical differences between control and drought stressed maize plants. However, most of them were affected by induction processes, relaxation rate, and/or differences in chlorophyll content; while they all were influenced by actinic light intensity on each light step of light curve. Only the normalized parameters related to photochemical and non-photochemical quenching were strongly correlated with data obtained by gas exchange, but only from the light step in which the linear electron flow reached saturation. Conclusions: The procedure developed in this study for discrimination of plant responses to water deficit stress proved to be as fast, efficient and reliable as the standard technique of gas exchange in order to discriminate the responses of maize genotypes to drought. However, unlike that, there is no need to perform daily and time consuming calibration routines. Moreover, plant acclimation to the dark is not required. The protocol can be applied to plants growing in both controlled conditions and full sunlight in the field. In addition, it generates parameters in a fast and accurate measurement process, which enables evaluating several plants in a short period of time. MenosBackground: Photosynthesis can be roughly separated into biochemical and photochemical processes. Both are affected by drought and can be assessed by non-invasive standard methods. Gas exchange, which mainly assesses the first process, has well-defined protocols. It is considered a standard method for evaluation of plant responses to drought. Under such stress, assessment of photochemical apparatus by chlorophyll fluorescence needs improvement to become faster and reproducible, especially in growing plants under field conditions. For this, we developed a protocol based on chlorophyll fluorescence imaging, using a rapid light curve approach. Results: Almost all parameters obtained by rapid light curves have shown statistical differences between control and drought stressed maize plants. However, most of them were affected by induction processes, relaxation rate, and/or differences in chlorophyll content; while they all were influenced by actinic light intensity on each light step of light curve. Only the normalized parameters related to photochemical and non-photochemical quenching were strongly correlated with data obtained by gas exchange, but only from the light step in which the linear electron flow reached saturation. Conclusions: The procedure developed in this study for discrimination of plant responses to water deficit stress proved to be as fast, efficient and reliable as the standard technique of gas exchange in order to discriminate the responses of maize genotypes... Mostrar Tudo |
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Palavras-Chave: |
Phenotyping; Water deficit; Zea mays L. |
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Thesaurus Nal: |
abiotic stress; corn; gas exchange; phenomics. |
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Categoria do assunto: |
--
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/168295/1/Procedure-maize.pdf
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Marc: |
LEADER 02834naa a2200313 a 4500
001 2081609
005 2018-02-15
008 2017 bl uuuu u00u1 u #d
024 7 $a10.1186/s13007-017-0209-z$2DOI
100 1 $aSOUSA, C. A. F. de
245 $aA procedure for maize genotypes discrimination to drought by chlorophyll fluorescence imaging rapid light curves.$h[electronic resource]
260 $c2017
520 $aBackground: Photosynthesis can be roughly separated into biochemical and photochemical processes. Both are affected by drought and can be assessed by non-invasive standard methods. Gas exchange, which mainly assesses the first process, has well-defined protocols. It is considered a standard method for evaluation of plant responses to drought. Under such stress, assessment of photochemical apparatus by chlorophyll fluorescence needs improvement to become faster and reproducible, especially in growing plants under field conditions. For this, we developed a protocol based on chlorophyll fluorescence imaging, using a rapid light curve approach. Results: Almost all parameters obtained by rapid light curves have shown statistical differences between control and drought stressed maize plants. However, most of them were affected by induction processes, relaxation rate, and/or differences in chlorophyll content; while they all were influenced by actinic light intensity on each light step of light curve. Only the normalized parameters related to photochemical and non-photochemical quenching were strongly correlated with data obtained by gas exchange, but only from the light step in which the linear electron flow reached saturation. Conclusions: The procedure developed in this study for discrimination of plant responses to water deficit stress proved to be as fast, efficient and reliable as the standard technique of gas exchange in order to discriminate the responses of maize genotypes to drought. However, unlike that, there is no need to perform daily and time consuming calibration routines. Moreover, plant acclimation to the dark is not required. The protocol can be applied to plants growing in both controlled conditions and full sunlight in the field. In addition, it generates parameters in a fast and accurate measurement process, which enables evaluating several plants in a short period of time.
650 $aabiotic stress
650 $acorn
650 $agas exchange
650 $aphenomics
653 $aPhenotyping
653 $aWater deficit
653 $aZea mays L
700 1 $aPAIVA, D S. de
700 1 $aCASARI, R. A. das C. N.
700 1 $aMOLINARI, H. B. C.
700 1 $aOLIVEIRA, N. G. de
700 1 $aKOBAYASHI, A. K.
700 1 $aMAGALHAES, P. C.
700 1 $aGOMIDE, R. L.
700 1 $aSOUZA JUNIOR, M. T.
773 $tPlant Methods$gv. 13, p. 1-17, 2017.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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Biblioteca |
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Origem |
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Cutter |
Registro |
Volume |
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Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
09/11/2004 |
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Data da última atualização: |
13/10/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
COIMBRA, R. R.; MIRANDA, G. V.; CRUZ, C, D.; SILVA, D. J. H.; VILELA, R. A. |
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Afiliação: |
Ronaldo R. Coimbra, Universidade Federal do Tocantins; Glauco V. Miranda, UFV; Cosme D. Cruz, UFV; Derly J. H. Silva, UFV; RAMIRO A. VILELA, CNPMS. |
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Título: |
Development of a Brazilian maize core collection. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
Genetics and Molecular Biology, Ribeirão Preto, v. 32, n. 3, p. 538-545, 2009. |
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DOI: |
10.1590/S1415-47572009005000059 |
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Idioma: |
Inglês |
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Conteúdo: |
The aim of this study was to evaluate methods for developing a Brazilian maize core collection. For an initial survey of the active collection, passport information, as well as characterization and evaluation of accessions, were taken into consideration, these then being divided according to geographic region and kernel-type. Multiple sampling methods were evaluated. The strategy of constant sampling generated extensive alterations in extract accession frequency. The multivariate strategy with dispersion graphs and principal components associated with the Tocher method was considered efficient for identifying the most divergent genotypes. The multivariate strategy generated greater alterations in the variance of traits. The average number of traits revealed few modifications with the various sampling strategies used. Therefore, the active collection could be considered as possessing a satisfactory amount of information for most of its accessions. Moreover, the multivariate strategy generated modifications in the variance of the traits, independent of sampling intensity. |
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Thesagro: |
Melhoramento; Milho. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/32578/1/Development-Brazilian.pdf
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Marc: |
LEADER 01687naa a2200205 a 4500
001 1488372
005 2017-10-13
008 2009 bl uuuu u00u1 u #d
024 7 $a10.1590/S1415-47572009005000059$2DOI
100 1 $aCOIMBRA, R. R.
245 $aDevelopment of a Brazilian maize core collection.$h[electronic resource]
260 $c2009
520 $aThe aim of this study was to evaluate methods for developing a Brazilian maize core collection. For an initial survey of the active collection, passport information, as well as characterization and evaluation of accessions, were taken into consideration, these then being divided according to geographic region and kernel-type. Multiple sampling methods were evaluated. The strategy of constant sampling generated extensive alterations in extract accession frequency. The multivariate strategy with dispersion graphs and principal components associated with the Tocher method was considered efficient for identifying the most divergent genotypes. The multivariate strategy generated greater alterations in the variance of traits. The average number of traits revealed few modifications with the various sampling strategies used. Therefore, the active collection could be considered as possessing a satisfactory amount of information for most of its accessions. Moreover, the multivariate strategy generated modifications in the variance of the traits, independent of sampling intensity.
650 $aMelhoramento
650 $aMilho
700 1 $aMIRANDA, G. V.
700 1 $aCRUZ, C, D.
700 1 $aSILVA, D. J. H.
700 1 $aVILELA, R. A.
773 $tGenetics and Molecular Biology, Ribeirão Preto$gv. 32, n. 3, p. 538-545, 2009.
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Embrapa Milho e Sorgo (CNPMS) |
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