Data/hora: 21/06/2024 10:27:23

Biblioteca(s): | Embrapa Unidades Centrais.
Data corrente: | 15/02/2016
Data da última atualização: | 15/02/2016
Autoria: | SIERRA-ARGUELLO, Y. M.; MORGAN, R. B.; PERDONCINI, G.; LIMA, L. M.; GOMES, M. J. P.; NASCIMENTO, V. P. do.
Afiliação: | YULI M. SIERRA-ARGUELLO, UFRGS; RAFAELA B. MORGAN, UFRGS; GUSTAVO PERDONCINI, UFRGS; LEORNADO M. LIMA, UFRGS; MARCOS JOSÉ P. GOMES, UFRGS; VLADIMIR PINHEIRO DO NASCIMENTO, UFRGS.
Título: | Resistence to B-lactams and tetracycline in Campuylobacter spp. isolated from broiler slaughterhouses in southern Brazil.
Ano de publicação: | 2015
Fonte/Imprenta: | Pesquisa Veterinária Brasileira v. 35, n.7, p. 637-642, julho 2015.
Idioma: | Inglês
Conteúdo: | The study was carried out to screen and analyze the genetic characteristics of antimicrobial resistance in Campylobacter spp. from poultry sources. A total of 141 strains of Campylobacter isolated from samples of broilers of slaughterhouses in southern Brazil was identified by phenotypic and genotypic methods. Campylobacter isolates were evaluated for its antimicrobial susceptibility and the presence of resistance genes. The strains were investigated for antimicrobial susceptibility against two agents (ampicillin and tetracycline) by disk diffusion method. PCR assay was used to confirm the specie and the presence of ampicillin (blaOXA-61), tetracycline tet(O), and the energy-dependent multi-drug efflux pump (cmeB) genes. Campylobacter jejuni was the most ubiquitous; its presence was determined in 140 samples out of 141 (99.3%), whereas Campylobacter coli was found only in one of the contaminated samples (0.70%). The results obtained showed 65% and 35.5% of Campylobacter isolates resistant to ?-lactams and tetracyclines, respectively. The cmeB gene responsible for multidrug resistance was detected in 26 isolates out 141 strains (18.5%). Moreover, 36 out of 141 Campylobacter strains (25.6%) were found to be resistant to at least two different antimicrobia resistance markers (?-lactams and tetracyclines).
Palavras-Chave: | Campylobacters; PCR; Resistence genes.
Thesaurus Nal: | Tetracycline; Transporters.
Categoria do assunto: | --
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/138967/1/Resistance-to-b-lactam.pdf
Registro original: | Embrapa Unidades Centrais (AI-SEDE)
Exemplares: | AI-SEDE - (58781-1) - UPE - AP - PP - 636.08905 - P474 -  - DIS