Data/hora: 25/06/2024 18:54:46

Biblioteca(s): 	Embrapa Milho e Sorgo.	
Data corrente: 	27/10/2010	
Data da última atualização: 	04/06/2018	
Autoria: 	GIARETTA, D. R.; BOGO, A.; COELHO, C. M. M.; GUIDOLIN, A. F.; DANTAS, A. C. de M.; GOMES, E. A.	
Afiliação: 	Diorvania Ribeiro Giaretta, UDESC; Amauri Bogo, UDESC; Cleide Maria Medeiros Coelho, UDESC; Altamir Frederico Guidolin, UDESC; Adriana Cibele de Mesquita Dantas, UFSC; ELIANE APARECIDA GOMES, CNPMS.	
Título: 	ITS-rDNA phylogeny of Colletotrichum spp. causal agent of apple glomerella leaf spot.	
Ano de publicação: 	2010	
Fonte/Imprenta: 	Ciência Rural, Santa Maria, v. 40, n. 4, p. 806-812, abr. 2010.	
DOI: 	10.1590/S0103-84782010005000051	
Idioma: 	Inglês	
Conteúdo: 	Several diseases have affected apple production, among them there is Glomerella leaf spot (GLS) caused by Colletotrichum spp. The first report of this disease in apple was in plants nearby citrus orchards in São Paulo State, Brazil. The origin of this disease is still not clear, and studies based on the molecular phylogeny could relate the organisms evolutionarily and characterize possible mechanisms of divergent evolution. The amplification of 5.8S-ITS (Internal Transcribed Spacer) of rDNA of 51 pathogenic Colletotrichum spp. isolates from apples, pineapple guava and citrus produced one fragment of approximately 600 bases pairs (bp) for all the isolates analyzed. The amplified fragments were cleaved with restriction enzymes, and fragments from 90 to 500bp were obtained. The sequencing of this region allowed the generation of a phylogenetic tree, regardless of their hosts, and 5 isolated groups were obtained. From the "in silico" comparison, it was possible to verify a variation from 93 to 100% of similarity between the sequences studied and the Genbank data base. The causal agent of GLS is nearly related (clustered) to isolates of pineapple guava and to the citrus isolates used as control.	
Palavras-Chave: 	Árvore filogenética; Filogenia molecular; Goiabeira serrana.	
Thesaurus Nal: 	Citrus.	
Categoria do assunto: 	--	
URL: 	http://ainfo.cnptia.embrapa.br/digital/bitstream/item/30974/1/its-rdna.pdf	
Registro original: 	Embrapa Milho e Sorgo (CNPMS)	
Exemplares: 	CNPMS - (23036-1) - UPC - AP - DD -  -  -  - DIS