Data/hora: 01/06/2024 17:41:15

Biblioteca(s): 	Embrapa Soja.	
Data corrente: 	16/01/2013	
Data da última atualização: 	03/08/2017	
Autoria: 	IKEDA, A. C.; BASSANI, L. L.; ADAMOSKI, D.; STRINGARI, D.; CORDEIRO, V. K.; GLIENKE, C.; STEFFENS, M. B. R.; HUNGRIA, M.; GALLI-TERASAWA, L. V.	
Afiliação: 	ANGELA CRISTINA IKEDA, UFPR; LUCIANA LANGE BASSANI, UFPR; DOUGLAS ADAMOSKI, UFPR; DANYELLE STRINGARI, UFPR; VANESSA KAVA CORDEIRO, UFPR; CHIRLEI GLIENKE, UFPR; MARIA BERENICE REYNAUD STEFFENS, UFPR; MARIANGELA HUNGRIA DA CUNHA, CNPSO; LIGIA VITORIA GALLI-TERASAWA, UFPR.	
Título: 	Morphological and genetic characterization of endophytic bacteria isolated from roots of different maize genotypes.	
Ano de publicação: 	2013	
Fonte/Imprenta: 	Microbial Ecology, New York, v. 65, n. 1, p. 154-160, 2013.	
ISSN: 	0095-3628	
DOI: 	10.1007/s00248-012-0104-0	
Idioma: 	Inglês	
Conteúdo: 	Maize is one of the most important crops worldwide, and in Brazil, the state of Paraná stands as its largest producer. The crop demands high inputs of N fertilizers, therefore all strategies aiming to optimize the grain production with lower inputs are very relevant. Endophytic bacteria have a high potential to increment maize grain yield by means of input via biological nitrogen fixation and/or plant growth promotion, in this last case increasing the absorption of water and nutrients by the plants. In this study, we established a collection of 217 endophytic bacteria, isolated from roots of four lineages and three hybrid genotypes of maize, and isolated in four different N-free culture media. Biochemical?comprising growth in different carbon sources, intrinsic tolerance to antibiotics, and biochemical tests for catalase, nitrate reductase, urease, and growth in N-free media in vitro?and genetic characterization by BOX-PCR revealed great variability among the isolates. Both commercial hybrids and homozygous lineages were broadly colonized by endophytes, and sequencing of the 16S rRNA gene revealed the presence of bacteria belonging to the genera Pantoea, Bacillus, Burkholderia,andKlebsiella.Qualitative differences in endophytic colonization were detected between lineages and hybrid genotypes.	
Thesagro: 	Fixação de nitrogênio; Milho.	
Thesaurus Nal: 	Corn; Nitrogen fixation.	
Categoria do assunto: 	S Ciências Biológicas	
Registro original: 	Embrapa Soja (CNPSO)	
Exemplares: 	CNPSO - (34077-1) - UPC - AP - DD -  -  -  - DIS