Data/hora: 22/06/2024 04:41:06

Biblioteca(s): | Embrapa Agrobiologia.
Data corrente: | 26/04/2000
Data da última atualização: | 12/11/2015
Autoria: | GLAUERT, A. M.
Título: | Fixation, dehydration and embedding of biological specimens.
Ano de publicação: | 1975
Fonte/Imprenta: | Amsterdan: North-Holland, 1975.
Páginas: | 207p.
Série: | Practical Methods em Electron Microscopy.
ISBN: | 0-7204-4250-8
Idioma: | Inglês
Conteúdo: | Electron microscopy of biological speciments; the need for ultrathin  sections. The aim of the procedure. Criteria of good preservation. Fixatives. The development of fixatives for electron microscopy. Vehicles for fixatives. Osmium tetroxide fixatives. Glutaraldehyde fixatives. Formaldehyde fixatives. Acrolein and other aldehyde fixatives. Permanganate fixatives. Mixed fixatives. Uranyl acetate fixatives. Fixation  methods. Time and temperature of fixation. Fixation schedules. Organs and tissues. Botanical specimens. Monolayers of cells. Isolated cells. Cell fractions. Methods for very small quantities of material. Special fixation schedules and methods. Dehydration. Chemical and morphological effects of dehydration. Dehydration schedules. Dehydrating agents. Embedding. Embedding media for electron microscopy. Standart embedding methods. Epoxy resins. Polyesyer resins. Methacrylates. Water-soluble embedding media. Other embedding media. Gelatin. Urea-aldehyde. Protein-aldehyde. Polyampholytes. Special embedding methods. Flat embedding. Monolayers of cells. Embedding methods for very small quantities of material. Low-temperature methods. Fixation, dehydration and embedding at low temperatures. Freeze-drying. Freeze-substituion. Cryo-ultramicrotomy. Commercial suppliers of equipment and materials for fixation, dehydration and embedding.
Palavras-Chave: | Methods; Microscopis eletronico.
Thesagro: | Método; Microscopia.
Thesaurus Nal: | microscopy.
Categoria do assunto: | --
Registro original: | Embrapa Agrobiologia (CNPAB)
Exemplares: | CNPAB - (25641-1) - ADC - RF - -- - 1997.00051 -  - 1997.00051 - DIS