Data/hora: 21/06/2024 03:28:51

Biblioteca(s): 	Embrapa Agrobiologia.	
Data corrente: 	27/12/2000	
Data da última atualização: 	27/12/2000	
Autoria: 	SALLES, J. F.; GITAHY, P. de M.; SKOT, L.; BALDANI, J. I.	
Título: 	Use of endophytic diazotrophic bacteria as a vector to express the cry3A from Bacillus thuringiensis.	
Ano de publicação: 	2000	
Fonte/Imprenta: 	Brazilian Journal of Microbiology, Brasilia, v. 31, p. 155-161, 2000.	
Idioma: 	Inglês	
Conteúdo: 	The goal of this study was to evaluate the potential of endophytic diazotrophic bacteria as a vector to express a cry gene from Bacillus thuringiensis, envisaging the control of pests that attack sugarcane plants. The endophytic nitrogen-fixing bacteria Gluconacetobacter diazotrophicus strain BR11281 and Herbaspirillum seropedicae strain BR11335 were used as models. The cry3A gene was transferred by conjugation using a suicide plasmid and the recombinant strains were selected by their ability to fix nitrogen in semi-solid N-free medium. The presence of the cry gene was detected by Southem-blot using an intemal fragment of 1.0 kb as a probe. The production of 8-endotoxin by the recombinant H. seropedicae strain was detected by dot blot while for G. diazotrophicus the Westem-blot technique was used. In both cases, a specific antibody raised against the B. thuringiensis toxin was applied. The 8-endotoxin production showed by the G. diazotrophicus recombinant strain was dependent on the nitrogen fixing conditions since the cry3A gene was fused to a nif promoter. In the case of H. seropedicae the 8-endotoxin expression was not affected by the promoter (rhi) used. These results suggest that endophytic diazotrophic bacteria can be used as vectors to express entomopathogenic genes envisaging control of sugarcane pests. 

Palavras-Chave: 	Bactéria diazotrófica endofítica; Endophytic diazotrophy bacteria.	
Thesagro: 	Bacillus Thuringiensis.	
Thesaurus Nal: 	Gluconacetobacter diazotrophicus; Herbaspirillum seropedicae.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Agrobiologia (CNPAB)	
Exemplares: 	CNPAB - (913-1) - UPC - SP - -- -  -  - 0022529 - DIS