Data/hora: 25/06/2024 14:29:00

Biblioteca(s): 	Embrapa Caprinos e Ovinos.	
Data corrente: 	01/08/1992	
Data da última atualização: 	30/11/2023	
Autoria: 	GIMENEZ, D. F.	
Título: 	Staining rickettsiae in yolk sac culture.	
Ano de publicação: 	1964	
Fonte/Imprenta: 	Stain Technology, v. 39, n. 3, p. 135-140, 1964.	
DOI: 	https://doi.org/10.3109/10520296409061219	
Idioma: 	Inglês	
Conteúdo: 	Abstract: Rickettsiae in yolk sacs are not stained well by the Macchiavello technique, and experiments were undertaken to understand the mechanisms involved. It was found that the citric acid destaining step was not effective and that most of the basic fuchsin was lost from the rickettsiae during the application of methylene blue, another basic dye. A staining technique was then evolved with carbol basic fuchsin in pH 7.45 phosphate buffer (0.4% dye, 0.4% phenol, 0.07 M buffer), followed directly by 0.8% aqueous malachite green oxalate. This technique worked well for R. mooseri, R. prowazeki, R. rickettsii, R. akari, and R. buretii, but for R. tsutsugumushi a modification was needed, whereby 4% aqueous Fe(NO3)3·9H2O was used as destaining solution, and 0.5% aqueous fast green as the counter-stain.	
Palavras-Chave: 	Coloring Agents; Staining and Labeling; Tissue Culture Techniques.	
Thesagro: 	Animal; Bacteriologia; Doença.	
Thesaurus Nal: 	Animal diseases; Culture media; Rickettsia; Yolk sac.	
Categoria do assunto: 	H Saúde e Patologia	
Registro original: 	Embrapa Caprinos e Ovinos (CNPC)	
Exemplares: 	CNPC - (9390-1) - ADD - AP - PP - SP 00113 -  - SP 00113 - DIS