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Registro Completo |
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Biblioteca(s): |
Embrapa Agricultura Digital; Embrapa Café. |
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Data corrente: |
05/02/2015 |
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Data da última atualização: |
06/02/2015 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CARDOSO, D. C.; MARTINATI, J. C.; GIACHETTO, P. F.; VIDAL, R. O.; CARAZZOLLE, M. F.; PADILHA, L.; GUERREIRO-FILHO, O.; MALUF, M. P. |
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Afiliação: |
DANIELLE C. CARDOSO, IAC; JULIANA C MARTINATI, IAC; POLIANA FERNANDA GIACHETTO, CNPTIA; RAMON O. VIDAL; MARCELO F. CARAZZOLLE; LILIAN PADILHA, SAPC; OLIVEIRO GUERREIRO-FILHO, IAC; MIRIAN PEREZ MALUF, SAPC. |
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Título: |
Large-scale analysis of differential gene expression in coffee genotypes resistant and susceptible to leaf miner-toward the identification of candidate genes for marker assisted-selection. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
BMC Genomics, London, v. 15, p. 1-20, 2014. |
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DOI: |
10.1186/1471-2164-15-66 |
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Idioma: |
Inglês |
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Conteúdo: |
A successful development of herbivorous insects into plant tissues depends on coordination of metabolic processes. Plants have evolved complex mechanisms to recognize such attacks, and to trigger a defense response. To understand the transcriptional basis of this response, we compare gene expression profiles of two coffee genotypes, susceptible and resistant to leaf miner (Leucoptera coffella). A total of 22000 EST sequences from the Coffee Genome Database were selected for a microarray analysis. Fluorescence probes were synthesized using mRNA from the infested and non-infested coffee plants. Array hybridization, scanning and data normalization were performed using Nimble Scan® e ArrayStar® platforms. Genes with foldchange values +/-2 were considered differentially expressed. A validation of 18 differentially expressed genes was performed in infected plants using qRT-PCR approach. |
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Palavras-Chave: |
Microarranjo; Plant defense. |
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Thesagro: |
Café; Coffea Arábica. |
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Thesaurus Nal: |
Genes; Leafminers; Microarray technology. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/117310/1/Large-scale-analysis.pdf
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Marc: |
LEADER 01841naa a2200301 a 4500
001 2007801
005 2015-02-06
008 2014 bl uuuu u00u1 u #d
024 7 $a10.1186/1471-2164-15-66$2DOI
100 1 $aCARDOSO, D. C.
245 $aLarge-scale analysis of differential gene expression in coffee genotypes resistant and susceptible to leaf miner-toward the identification of candidate genes for marker assisted-selection.$h[electronic resource]
260 $c2014
520 $aA successful development of herbivorous insects into plant tissues depends on coordination of metabolic processes. Plants have evolved complex mechanisms to recognize such attacks, and to trigger a defense response. To understand the transcriptional basis of this response, we compare gene expression profiles of two coffee genotypes, susceptible and resistant to leaf miner (Leucoptera coffella). A total of 22000 EST sequences from the Coffee Genome Database were selected for a microarray analysis. Fluorescence probes were synthesized using mRNA from the infested and non-infested coffee plants. Array hybridization, scanning and data normalization were performed using Nimble Scan® e ArrayStar® platforms. Genes with foldchange values +/-2 were considered differentially expressed. A validation of 18 differentially expressed genes was performed in infected plants using qRT-PCR approach.
650 $aGenes
650 $aLeafminers
650 $aMicroarray technology
650 $aCafé
650 $aCoffea Arábica
653 $aMicroarranjo
653 $aPlant defense
700 1 $aMARTINATI, J. C.
700 1 $aGIACHETTO, P. F.
700 1 $aVIDAL, R. O.
700 1 $aCARAZZOLLE, M. F.
700 1 $aPADILHA, L.
700 1 $aGUERREIRO-FILHO, O.
700 1 $aMALUF, M. P.
773 $tBMC Genomics, London$gv. 15, p. 1-20, 2014.
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Registro original: |
Embrapa Agricultura Digital (CNPTIA) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
19/12/2018 |
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Data da última atualização: |
24/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
DIAS, A. T.; CASTRO, S. B. R. de; ALVES, C. C. de S.; EVANGELISTA, M. G.; SILVA, L. C. da; REIS, D. R. de L.; MACHADO, M. A.; JULIANO, M. A.; FERREIRA, A. P. |
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Afiliação: |
ALYRIA TEIXEIRA DIAS, UFJF; SANDRA BERTELLI RIBEIRO DE CASTRO, UFJF; CAIO CÉSAR DE SOUZA ALVES, Universidade Federal dos Vales do Jequitinhonha e Mucuri, Teófilo Otoni; MARCILENE GOMES EVANGELISTA, UFJF; LUAN CRISTIAN DA SILVA, UFJF; DANIELE RIBEIRO DE LIMA REIS FAZA, CNPGL; MARCO ANTONIO MACHADO, CNPGL; MARIA APARECIDA JULIANO, Universidade Federal de São Paulo; ANA PAULA FERREIRA, UFJF. |
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Título: |
Genistein modulates the expression of Toll-like receptors in experimental autoimmune encephalomyelitis. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Inflammation Research, v. 67, n. 7, p. 597-608, 2018. |
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DOI: |
10.1007/s00011-018-1146-7 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract OBJECTIVE AND DESIGN: The present work investigates the modulation of experimental autoimmune encephalomyelitis (EAE) using genistein before the EAE induction. MATERIAL: Female C57BL/6 mice (n = 96 mice/experiment), 4-6 weeks old, were used to induce the EAE. The mice were divided into three experimental groups: non-immunized group, immunized group (EAE), and immunized and treated with genistein group (Genistein). TREATMENT: Genistein was used at a dose of 200 mg/kg s.c. and were initiated 2 days before the immunization and continued daily until day 6 postimmunization. METHODS: Animals were monitored daily for clinical signs of EAE up to day 21. Inflammatory infiltration, demyelination, Toll-like receptor (TLR) expression, cytokines and transcription factors were analyzed in spinal cords. RESULTS: The present study demonstrates, for the first time, the genistein ability to modulate the factors involved in the innate immune response in the early stages of EAE. The genistein therapy delayed the onset of the disease, with reduced inflammatory infiltration and demyelination. In addition, the expression of TLR3, TLR9 and IFN-β were increased in genistein group, with reduction in the factors of TH1 and Th17 cells. CONCLUSION: These findings shed light on the potential of genistein as a prophylactic strategy for multiple sclerosis (MS) prevention. |
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Palavras-Chave: |
Experimental autoimmune encephalomyelitis; IFN-beta; Multiple sclerosis; Toll-like. |
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Thesaurus NAL: |
Immune response. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02278naa a2200289 a 4500
001 2102082
005 2023-01-24
008 2018 bl uuuu u00u1 u #d
024 7 $a10.1007/s00011-018-1146-7$2DOI
100 1 $aDIAS, A. T.
245 $aGenistein modulates the expression of Toll-like receptors in experimental autoimmune encephalomyelitis.$h[electronic resource]
260 $c2018
520 $aAbstract OBJECTIVE AND DESIGN: The present work investigates the modulation of experimental autoimmune encephalomyelitis (EAE) using genistein before the EAE induction. MATERIAL: Female C57BL/6 mice (n = 96 mice/experiment), 4-6 weeks old, were used to induce the EAE. The mice were divided into three experimental groups: non-immunized group, immunized group (EAE), and immunized and treated with genistein group (Genistein). TREATMENT: Genistein was used at a dose of 200 mg/kg s.c. and were initiated 2 days before the immunization and continued daily until day 6 postimmunization. METHODS: Animals were monitored daily for clinical signs of EAE up to day 21. Inflammatory infiltration, demyelination, Toll-like receptor (TLR) expression, cytokines and transcription factors were analyzed in spinal cords. RESULTS: The present study demonstrates, for the first time, the genistein ability to modulate the factors involved in the innate immune response in the early stages of EAE. The genistein therapy delayed the onset of the disease, with reduced inflammatory infiltration and demyelination. In addition, the expression of TLR3, TLR9 and IFN-β were increased in genistein group, with reduction in the factors of TH1 and Th17 cells. CONCLUSION: These findings shed light on the potential of genistein as a prophylactic strategy for multiple sclerosis (MS) prevention.
650 $aImmune response
653 $aExperimental autoimmune encephalomyelitis
653 $aIFN-beta
653 $aMultiple sclerosis
653 $aToll-like
700 1 $aCASTRO, S. B. R. de
700 1 $aALVES, C. C. de S.
700 1 $aEVANGELISTA, M. G.
700 1 $aSILVA, L. C. da
700 1 $aREIS, D. R. de L.
700 1 $aMACHADO, M. A.
700 1 $aJULIANO, M. A.
700 1 $aFERREIRA, A. P.
773 $tInflammation Research$gv. 67, n. 7, p. 597-608, 2018.
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Embrapa Gado de Leite (CNPGL) |
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