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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
27/10/2015 |
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Data da última atualização: |
18/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
DÓRIA, M. S.; SOUSA, A. O. de; BARBOSA, C. de J.; COSTA, M. G. C.; GESTEIRA, A. da S.; SOUZA, R. M.; FREITAS, A. C. O.; PIROVANI, C. P. |
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Afiliação: |
MILENA SANTOS DÓRIA, UESC; AURIZÂNGELA OLIVEIRA DE SOUSA, UESC; CRISTIANE DE JESUS BARBOSA, CNPMF; MÁRCIO GILBERTO CARDOSO COSTA, UESC; ABELMON DA SILVA GESTEIRA, CNPMF; REGINA MARTINS SOUZA; ANA CAMILA OLIVEIRA FREITAS, UESC; CARLOS PRIMINHO PIROVANI, UESC. |
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Título: |
Citrus tristeza virus (CTV) causing proteomic and enzymatic changes in sweet orange variety "Westin". |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Plos One, July, 2015. |
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ISSN: |
1932-6203 |
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Idioma: |
Inglês |
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Conteúdo: |
Citrus Tristeza disease, caused by CTV (Citrus tristeza virus), committs citrus plantations around the world and specifically attacks phloem tissues of the plant. The virus exists as a mixture of more or less severe variants, which may or may not cause symptoms of Tristeza. The objective of this study was to analyze the changes caused by CTV in the proteome of stems of sweet orange, as well as in the activity and gene expression of antioxidant enzymes. The CTV-infected sweet orange displayed mild symptoms, which were characterized by the presence of sparse stem pitting throughout their stems. The presence of virus was confirmed by RT-PCR. Proteomic analysis by 2DE-PAGE-MS / MS revealed the identity of 40 proteins differentially expressed between CTV- infected and -non-infected samples. Of these, 33 were up-regulated and 7 were down-regulated in CTV-infected samples. Among the proteins identified stands out a specific from the virus, the coat protein. Other proteins identified are involved with oxidative stress and for this their enzymatic activity was measured. The activity of superoxide dismutase (SOD) was higher in CTV-infected samples, as catalase (CAT) showed higher activity in uninfected samples. The activity of guaiacol peroxidase (GPX) did not vary significantly between samples. However, ascorbate peroxidase (APX) was more active in the infected samples. The relative expression of the genes encoding CAT, SOD, APX and GPX was analyzed by quantitative real time PCR (RTqPCR). The CTV-infected samples showed greater accumulation of transcripts, except for the CAT gene. This gene showed higher expression in the uninfected samples. Taken together, it can be concluded that the CTV affects the protein profile and activity and gene expression of antioxidant enzymes in plants infected by this virus. MenosCitrus Tristeza disease, caused by CTV (Citrus tristeza virus), committs citrus plantations around the world and specifically attacks phloem tissues of the plant. The virus exists as a mixture of more or less severe variants, which may or may not cause symptoms of Tristeza. The objective of this study was to analyze the changes caused by CTV in the proteome of stems of sweet orange, as well as in the activity and gene expression of antioxidant enzymes. The CTV-infected sweet orange displayed mild symptoms, which were characterized by the presence of sparse stem pitting throughout their stems. The presence of virus was confirmed by RT-PCR. Proteomic analysis by 2DE-PAGE-MS / MS revealed the identity of 40 proteins differentially expressed between CTV- infected and -non-infected samples. Of these, 33 were up-regulated and 7 were down-regulated in CTV-infected samples. Among the proteins identified stands out a specific from the virus, the coat protein. Other proteins identified are involved with oxidative stress and for this their enzymatic activity was measured. The activity of superoxide dismutase (SOD) was higher in CTV-infected samples, as catalase (CAT) showed higher activity in uninfected samples. The activity of guaiacol peroxidase (GPX) did not vary significantly between samples. However, ascorbate peroxidase (APX) was more active in the infected samples. The relative expression of the genes encoding CAT, SOD, APX and GPX was analyzed by quantitative real time PCR (RTq... Mostrar Tudo |
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Palavras-Chave: |
Plant disease. |
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Thesagro: |
Doença de planta; Fruta cítrica; Vírus. |
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Thesaurus Nal: |
Citrus. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 02591naa a2200277 a 4500
001 2027385
005 2023-05-18
008 2015 bl uuuu u00u1 u #d
022 $a1932-6203
100 1 $aDÓRIA, M. S.
245 $aCitrus tristeza virus (CTV) causing proteomic and enzymatic changes in sweet orange variety "Westin".$h[electronic resource]
260 $c2015
520 $aCitrus Tristeza disease, caused by CTV (Citrus tristeza virus), committs citrus plantations around the world and specifically attacks phloem tissues of the plant. The virus exists as a mixture of more or less severe variants, which may or may not cause symptoms of Tristeza. The objective of this study was to analyze the changes caused by CTV in the proteome of stems of sweet orange, as well as in the activity and gene expression of antioxidant enzymes. The CTV-infected sweet orange displayed mild symptoms, which were characterized by the presence of sparse stem pitting throughout their stems. The presence of virus was confirmed by RT-PCR. Proteomic analysis by 2DE-PAGE-MS / MS revealed the identity of 40 proteins differentially expressed between CTV- infected and -non-infected samples. Of these, 33 were up-regulated and 7 were down-regulated in CTV-infected samples. Among the proteins identified stands out a specific from the virus, the coat protein. Other proteins identified are involved with oxidative stress and for this their enzymatic activity was measured. The activity of superoxide dismutase (SOD) was higher in CTV-infected samples, as catalase (CAT) showed higher activity in uninfected samples. The activity of guaiacol peroxidase (GPX) did not vary significantly between samples. However, ascorbate peroxidase (APX) was more active in the infected samples. The relative expression of the genes encoding CAT, SOD, APX and GPX was analyzed by quantitative real time PCR (RTqPCR). The CTV-infected samples showed greater accumulation of transcripts, except for the CAT gene. This gene showed higher expression in the uninfected samples. Taken together, it can be concluded that the CTV affects the protein profile and activity and gene expression of antioxidant enzymes in plants infected by this virus.
650 $aCitrus
650 $aDoença de planta
650 $aFruta cítrica
650 $aVírus
653 $aPlant disease
700 1 $aSOUSA, A. O. de
700 1 $aBARBOSA, C. de J.
700 1 $aCOSTA, M. G. C.
700 1 $aGESTEIRA, A. da S.
700 1 $aSOUZA, R. M.
700 1 $aFREITAS, A. C. O.
700 1 $aPIROVANI, C. P.
773 $tPlos One, July, 2015.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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| Registros recuperados : 178 | |
| 102. |  | GOMES, L. M. C.; GESTEIRA, A. da S.; SOUZA, V. L.; ALMEIDA, A-A. F. de; GOMES, F. P.; MEDEIROS, L. L. Trocas gasosas em espécies arbóreas submetidas ao estresse por cádmio. In: CONGRESSO BRASILEIRO DE FISIOLOGIA VEGETAL, 12., 2009, Fortaleza. Desafios para a produção de alimentos e bioenergia. Fortaleza: UFC: Embrapa Agroindústria Tropical, 2009. 1 CD-ROM. pdf 274.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 104. | | CUNHA, E. C.; ANJOS, P. S. dos; LINO, L. S. M.; SILVA, S. da C.; SOARES FILHO, W. dos S.; GESTEIRA, A. da S. Viabilidade e germinação in vitro de grãos de pólen da limeira acida Tahiti 2000 (CITRUS LATIFOLlA TANAKA). In: REUNIÃO ANUAL DE CIÊNCIA, TECNOLOGIA, INOVAÇÃO E CULTURA NO RECÔNCAVO DA BAHIA - RECONCITEC, 2., 2012, Cruz das Almas. Energias Renováveis, Educação, Tecnologia e Sociedade. Cruz das Almas, BA: UFRB, 2012.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
|  |
| 105. | | SOUZA, E. dos S.; JUNGHANS, T. G.; SANTANA, L. G. L.; SOUZA, A. da S.; GESTEIRA, A. da S.; SOARES FILHO, W. dos S. Variedades de tangerineira e potencial de obtenção de triploides com frutos sem sementes. In: JORNADA CIENTÍFICA EMBRAPA MANDIOCA E FRUTICULTURA, 6., 2012, Cruz das Almas. Anais... Cruz das Almas: Embrapa Mandioca e Fruticultura, 2012.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
|  |
| 106. | | SANTANA, L. G. L.; SOUZA, E. dos S.; PASSOS, O. S.; GESTEIRA, A. da S.; LEDO, C. A. da S.; SOARES FILHO, W. dos S. Vigor and mortality of citrus progenies with potential use as rootstocks. Scientia Agricola, v.75, n.4, p.339-345, July/August 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 107. | | FADINI, M.; GIRARDI, E. A.; SANTOS, M. G.; GESTEIRA, A. da S.; PASSOS, O. S.; SOUZA, F. V. D.; SOARES FILHO, W. dos S. Scion and rootstock combinations for ornamental citrus in containers. In: INTERNATIONAL CITRUS CONGRESS, 22., 2012.Valencia. Book of abstracts. Valencia: International Society of Citriculture; Instituto Valenciano de Investigaciones Agrarias; Fundación Agroalimed, 2012. Documento eletrônico.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
| |
| 108. | | MASCARENHAS JUNIOR, F. P.; COELHO FILHO, M. A.; AGUIAR, S. A.; MICHELI, F. F. L.; GESTEIRA, A. da S.; FANCELLI, M. Respostas fisiológicas de plantas cítricas submetidas ao estresse hídrico e infestação por Diaphorina citri. In: JORNADA CIENTÍFICA EMBRAPA MANDIOCA E FRUTICULTURA, 8., 2014, Cruz das Almas, Ba. Pesquisa: despertando mentes para a inovação e transformando o futuro: [anais]. Cruz das Almas, BA, Embrapa Mandioca e Fruticultura, 2014.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 109. | | SANTIAGO, R. T.; SANTOS, K. C. F. dos; LEDO, C. A. da S.; GESTEIRA, A. da S.; SOARES FILHO, W. dos S.; SOUZA, A. da S. Micropropagation of different citrus rootstocks using WPM medium culture. Journal of Agricultural Science, v. 11, n, 4, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 110. | | DUTRA, A. D.; COELHO FILHO, M. A.; PISSINATO, A. G. V.; GESTEIRA, A. da S.; SOARES FILHO, W. dos S.; FANCELLI, M. Mathematical models to estimate leaf area of citrus genotypes. African Journal of Agricultural Research, v. 12, n.2, p. 125-132, January, 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 111. | | OLIVEIRA, R. P. de; SOARES FILHO, W. dos S.; MACHADO, M. A.; FERREIRA, E. A.; SCIVITTARO, W. B.; GESTEIRA, A. da S. Melhoramento genético de plantas cítricas. Informe Agropecuário , Belo Horizonte , v. 3 5 , n . 2 8 1 , p . 2 2 - 2 9 , j u l . / a g o . 2 0 1 4.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 4 |
| Biblioteca(s): Embrapa Clima Temperado; Embrapa Mandioca e Fruticultura. |
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| 112. | | BRITTO, D. S.; PIROVANI, C. P.; GONZALEZ, E. R.; SILVA, J. F.; GESTEIRA, A. da S.; CASCARDO, J. C. de M. Oxidative stress proteins as an indicator of a low quality of eucalyptus clones for the pulp and paper industry. Genetics and Molecular Research, v. 11, n. 4, p. 3798-3813, 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 113. | | FRANÇA, N. de O.; GIRARDI, E. A.; AMORIM, M. da S.; GESTEIRA, A. da S.; PASSOS, O. S.; SOARES FILHO, W. dos S. Plant growth, yield and fruit quality of 'Piemonte' tangor grafted onto 14 rootstocks on the northern coast of the state of Bahia, Brazil. Revista Brasileira de Fruticultura, v.40 n.4 Jaboticabal, 2018. (e-784).| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 115. | | VIEIRA, D. D. S. S.; EMILIANI, G.; BARTOLINI, P.; PODDA, A.; CENTRITTO, M.; LURO, F.; CARRATORE, R. D.; MORILLON, R.; GESTEIRA, A. da S.; MASERTI, B. A L-type lectin gene is involved in the response to hormonal treatment and water deficit in Volkamer lemon. Journal of Plant Physiology, v.218, p.94-99, 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 116. | | BRITTO, D. S.; PIROVANI, C. P.; ANDRADE, B. S.; SANTOS, T. P. dos; PUNGARTNIK, C.; CASCARDO, J. C. M.; MICHELI, F.; GESTEIRA, A. da S. Recombinant b-1,3-1,4-glucanase from Theobroma cacao impairs Moniliophthora perniciosa mycelial growth. Molecular Biology Reports, 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 118. | | ALMEIDA, L. A. da H.; SOUZA, D. D.; SANTOS, N. de A.; SCHUSTER, I.; SOARES FILHO, W. dos S.; COELHO FILHO, M. A.; GESTEIRA, A. da S. Water deficit increases the frequency of hybrid citrus with polyembryonic female parents Water deficit increases the frequency of hybrid citrus with polyembryonic female parents. Crop Breeding and Applied Biotechnology, v.18, p. 47-54, 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 119. | | SAMPAIO, A. H. R.; COELHO FILHO, M. A.; SOUZA, L. D.; BRITO, R. B. F.; SILVA, R. O.; SOARES FILHO, W. dos S.; GESTEIRA, A. da S. Water relations and yield of Pera sweet orange grafted on different rootstocks. In: WORKSHOP ON BIOTIC AND ABIOTIC STRESS TOLERANCE IN PLANTS, 2013, Ilhéus. The challenge for the 21st century: book of abstracts. [S.l.]: International Advanced Biology Consortium, 2013. Online. S02P08.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 120. | | NEVES, D. M.; COELHO FILHO, M. A.; BELLETE, B. S.; SILVA, M. F. G. F.; SOUZA, D. T.; SOARES FILHO, W. dos S.; COSTA, M. G. C.; GESTEIRA, A. da S. The adaptation of Sunki Maravilha mandarin to drought depends on the ABA accumulation balance between roots and canopy. In: INTERNATIONAL CITRUS CONGRESS, 22., 2012.Valencia. Book of abstracts. Valencia: International Society of Citriculture; Instituto Valenciano de Investigaciones Agrarias; Fundación Agroalimed, 2012. Documento eletrônico.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| Registros recuperados : 178 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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