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Registro Completo |
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Biblioteca(s): |
Embrapa Uva e Vinho. |
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Data corrente: |
31/01/2011 |
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Data da última atualização: |
20/05/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MALAUSA, T.; FENNIS, A.; WAROT, S.; GERMAIN, J.-F.; RIS, N.; PRADO, E.; BOTTON, M.; VANLERBERGHE-MASUTTI, F.; SFORZA, R.; CRUAUD, C.; COULOUX, A.; KREITER, P. |
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Afiliação: |
TIBAULT MALAUSA, INRA; A. FENNIS, INRA; S. WAROT, INRA; J.-F. GERMAIN, LNPV MONTPELLIER; N. RIS, INRA; E. PRADO, EPAMIG; MARCOS BOTTON, CNPUV; F. VANLERBERGHE-MASUTTI, INRA; R. SFORZA, EUROPEAN BIOLOGICAL CONTROL LABORATORY; C. CRUAUD, GÉNOSCOPE; A. COULOUX, GÉNOSCOPE; P. KREITER, INRA. |
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Título: |
DNA markers to disentangle complexes of cryptic taxa in mealybugs (Hemiptera: Pseudococcidae). |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Journal of Applied Entomology, Hamburg, v. 135, n. 1/2, p. 142-155, fev. 2011. |
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Volume: |
135 |
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Páginas: |
142-155 |
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DOI: |
10.1111/j.1439-0418.2009.01495.x |
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Idioma: |
Inglês |
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Conteúdo: |
Mealybugs (Hemiptera: Pseudococcidae) are major pests of a wide range of crops and ornamental plants worldwide. Their high degree of morphological similarity makes them difficult to identify and limits their study and management. We aimed to identify a set of markers for the genetic characterization and identification of complexes of taxa in the Pseudococcidae. We surveyed and tested the genetic markers used in previous studies and then identified new markers for particularly relevant genomic regions for which no satisfactory markers were available. We tested all markers on a subset of four taxa distributed worldwide. Five markers were retained after this first screening: two regions of the mitochondrial cytochrome oxidase I gene, 28S-D2, the entire internal transcriber space 2 locus and the rpS15-16S region of the primary mealybug endosymbiont Tremblaya princeps. We then assessed the utility of these markers for the characterization and identification of 239 samples from 43 sites in France and Brazil. The five markers studied (i) successfully distinguished all species identified by morphological examination, (ii) disentangled complexes of species by revealing intraspecific genetic variation and identified a set of closely related taxa for which taxonomic status requires clarification through further studies, and (iii) facilitated the inference of phylogenetic relationships between the characterized taxa. |
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Palavras-Chave: |
Caracterização molecular; Identificação genética. |
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Thesagro: |
Cochonilha; DNA; Entomologia; Genética; Inseto; Marcador Molecular. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/26169/1/Botton-JAppliedEntomology-2011.pdf
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Marc: |
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008 2011 bl uuuu u00u1 u #d
024 7 $a10.1111/j.1439-0418.2009.01495.x$2DOI
100 1 $aMALAUSA, T.
245 $aDNA markers to disentangle complexes of cryptic taxa in mealybugs (Hemiptera$bPseudococcidae).$h[electronic resource]
260 $c2011
300 $a142-155 135
490 $v135
520 $aMealybugs (Hemiptera: Pseudococcidae) are major pests of a wide range of crops and ornamental plants worldwide. Their high degree of morphological similarity makes them difficult to identify and limits their study and management. We aimed to identify a set of markers for the genetic characterization and identification of complexes of taxa in the Pseudococcidae. We surveyed and tested the genetic markers used in previous studies and then identified new markers for particularly relevant genomic regions for which no satisfactory markers were available. We tested all markers on a subset of four taxa distributed worldwide. Five markers were retained after this first screening: two regions of the mitochondrial cytochrome oxidase I gene, 28S-D2, the entire internal transcriber space 2 locus and the rpS15-16S region of the primary mealybug endosymbiont Tremblaya princeps. We then assessed the utility of these markers for the characterization and identification of 239 samples from 43 sites in France and Brazil. The five markers studied (i) successfully distinguished all species identified by morphological examination, (ii) disentangled complexes of species by revealing intraspecific genetic variation and identified a set of closely related taxa for which taxonomic status requires clarification through further studies, and (iii) facilitated the inference of phylogenetic relationships between the characterized taxa.
650 $aCochonilha
650 $aDNA
650 $aEntomologia
650 $aGenética
650 $aInseto
650 $aMarcador Molecular
653 $aCaracterização molecular
653 $aIdentificação genética
700 1 $aFENNIS, A.
700 1 $aWAROT, S.
700 1 $aGERMAIN, J.-F.
700 1 $aRIS, N.
700 1 $aPRADO, E.
700 1 $aBOTTON, M.
700 1 $aVANLERBERGHE-MASUTTI, F.
700 1 $aSFORZA, R.
700 1 $aCRUAUD, C.
700 1 $aCOULOUX, A.
700 1 $aKREITER, P.
773 $tJournal of Applied Entomology, Hamburg$gv. 135, n. 1/2, p. 142-155, fev. 2011.
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Registro original: |
Embrapa Uva e Vinho (CNPUV) |
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| Registros recuperados : 5 | |
| 1. |  | SILVA, V. P. da; KAYDAN, M. B.; MALAUSA, T.; GERMAIN, J. F.; PALERO, F.; BOTTON, M. Integrative taxonomy methods reveal high mealybug (Hemiptera: Pseudococcidae) diversity in southern Brazilian fruit crops. Scientific Reports, v. 7, n. 15741, p. 1-9, 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Uva e Vinho. |
|    |
| 2. | | SILVA, V. C. P. da; MALAUSA, T.; GERMAIN, J-F.; BOTTON, M.; KAYDAN, M. B. Mealybugs (Hemiptera: Pseudococcidae) associated with persimmon Diospyros kaki L. (Ebenaceae) in Southern Brazil. In: INTERNAIONAL SYMPOSIUM ON SCALE INSECT STUDIES, 14., 2016, Catania, Italy. Anais... Catania, Italy: ISSIS, 2016.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 4. | | SILVA, V. C. P. da; BERTIN, A.; BLIN, A.; GERMAIN, J.-F.; BERNARDI, D.; RIGNOL, G.; BOTTON, M.; MALAUSA, T. Molecular and morphological identification of mealybug species (Hemiptera: Pseudococcidae) in Brazilian vineyards. Plos One, v. 9, n. 7, Jul. 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 5. | | MALAUSA, T.; FENNIS, A.; WAROT, S.; GERMAIN, J.-F.; RIS, N.; PRADO, E.; BOTTON, M.; VANLERBERGHE-MASUTTI, F.; SFORZA, R.; CRUAUD, C.; COULOUX, A.; KREITER, P. DNA markers to disentangle complexes of cryptic taxa in mealybugs (Hemiptera: Pseudococcidae). Journal of Applied Entomology, Hamburg, v. 135, n. 1/2, p. 142-155, fev. 2011. 135 142-155| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| Registros recuperados : 5 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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