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Registro Completo |
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Biblioteca(s): |
Embrapa Agricultura Digital. |
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Data corrente: |
09/01/2020 |
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Data da última atualização: |
04/02/2020 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
ZANELLA, L.; DEGENHARDT-GOLDBACH, J.; GERHARDT, I. R.; BESPALHOK FILHO, J. C. |
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Afiliação: |
LAUDIANE ZANELLA, UFPR; JULIANA DEGENHARDT GOLDBACH, CNPF; ISABEL RODRIGUES GERHARDT, CNPTIA; JOÃO CARLOS BESPALHOK FILHO, UFPR. |
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Título: |
RNA interference supression of DOF transcription factor from Populus tremula x Populus alba. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Pesquisa Florestal Brasileira, v. 39, e201902043, p. 322, 2019. |
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Idioma: |
Inglês |
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Notas: |
Na publicação: Isabel Gerhardt. Edição especial dos resumos do IUFRO World Congress, 25., 2019, Curitiba. |
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Conteúdo: |
From the total biomass produced by plants, 70% is represented by cell wall, in which carbon is mainly accumulated in the secondary wall. Therefore, the understanding of the mechanisms that regulate cell wall synthesis and its modification through genetic engineering may lead to the increase of lignocellulosic biomass. Among several functions already attributed to the proteins of the Dof transcription factors, its relation with cell wall formation is one of the most studied. However, the mechanisms involved in this process are not fully elucidated. Populus is the most used model plant for studies with forest species, including functional genomics. The objective of this work was to silence one gene of the Dof family by RNAi in plants of Populus tremula x P. alba, and investigate its function, aiming at the future production of Eucalyptus and Pinus plants with higher biomass production. For this, a silencing vector was constructed with specific primers of a Dof family gene from Populus. To obtain the transgenic plants via Agrobacterium tumefaciens, root segments were used as initial explants. Fifteen events were obtained, and in twelve the Dof expression was suppressed at different levels, according to qPCR analyzes. Of these, on five the expression was highly suppressed, indicating that the gene was successfully silenced. In morphological evaluations after 90 days the events did not present phenotypic differences in relation to non transformed plants. Physiological and histochemical analyzes are underway to evaluate lignin and cellulose contents and structural changes in transformed plants. MenosFrom the total biomass produced by plants, 70% is represented by cell wall, in which carbon is mainly accumulated in the secondary wall. Therefore, the understanding of the mechanisms that regulate cell wall synthesis and its modification through genetic engineering may lead to the increase of lignocellulosic biomass. Among several functions already attributed to the proteins of the Dof transcription factors, its relation with cell wall formation is one of the most studied. However, the mechanisms involved in this process are not fully elucidated. Populus is the most used model plant for studies with forest species, including functional genomics. The objective of this work was to silence one gene of the Dof family by RNAi in plants of Populus tremula x P. alba, and investigate its function, aiming at the future production of Eucalyptus and Pinus plants with higher biomass production. For this, a silencing vector was constructed with specific primers of a Dof family gene from Populus. To obtain the transgenic plants via Agrobacterium tumefaciens, root segments were used as initial explants. Fifteen events were obtained, and in twelve the Dof expression was suppressed at different levels, according to qPCR analyzes. Of these, on five the expression was highly suppressed, indicating that the gene was successfully silenced. In morphological evaluations after 90 days the events did not present phenotypic differences in relation to non transformed plants. Physiological and histoch... Mostrar Tudo |
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Thesaurus Nal: |
Populus alba; Populus tremula. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02291nam a2200181 a 4500
001 2118433
005 2020-02-04
008 2019 bl uuuu u01u1 u #d
100 1 $aZANELLA, L.
245 $aRNA interference supression of DOF transcription factor from Populus tremula x Populus alba.$h[electronic resource]
260 $aPesquisa Florestal Brasileira, v. 39, e201902043, p. 322$c2019
500 $aNa publicação: Isabel Gerhardt. Edição especial dos resumos do IUFRO World Congress, 25., 2019, Curitiba.
520 $aFrom the total biomass produced by plants, 70% is represented by cell wall, in which carbon is mainly accumulated in the secondary wall. Therefore, the understanding of the mechanisms that regulate cell wall synthesis and its modification through genetic engineering may lead to the increase of lignocellulosic biomass. Among several functions already attributed to the proteins of the Dof transcription factors, its relation with cell wall formation is one of the most studied. However, the mechanisms involved in this process are not fully elucidated. Populus is the most used model plant for studies with forest species, including functional genomics. The objective of this work was to silence one gene of the Dof family by RNAi in plants of Populus tremula x P. alba, and investigate its function, aiming at the future production of Eucalyptus and Pinus plants with higher biomass production. For this, a silencing vector was constructed with specific primers of a Dof family gene from Populus. To obtain the transgenic plants via Agrobacterium tumefaciens, root segments were used as initial explants. Fifteen events were obtained, and in twelve the Dof expression was suppressed at different levels, according to qPCR analyzes. Of these, on five the expression was highly suppressed, indicating that the gene was successfully silenced. In morphological evaluations after 90 days the events did not present phenotypic differences in relation to non transformed plants. Physiological and histochemical analyzes are underway to evaluate lignin and cellulose contents and structural changes in transformed plants.
650 $aPopulus alba
650 $aPopulus tremula
700 1 $aDEGENHARDT-GOLDBACH, J.
700 1 $aGERHARDT, I. R.
700 1 $aBESPALHOK FILHO, J. C.
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Registro original: |
Embrapa Agricultura Digital (CNPTIA) |
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| Registros recuperados : 6 | |
| 4. |  | SILVA, L. M. A. e; CAVALCANTE, J. M.; RIBEIRO, P. R. V.; CANUTO, K. M.; BRITO, E. S. de. Uso da cromatografia flash como método alternativo e de baixo custo para a separação dos principais componentes do LCC. In: ENCONTRO NACIONAL SOBRE METODOLOGIAS E GESTÃO DE LABORATÓRIOS DA EMBRAPA, 19.; SIMPÓSIO SOBRE PROCEDIMENTOS ANALÍTICOS E A RASTREABILIDADE DOS RESULTADOS DA AGROPECUÁRIA, 6., 2014, Fortaleza, CE. Unir talentos para ultrapassar fronteiras: resumos. Fortaleza: Embrapa Agroindústria Tropical, 2014.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroindústria Tropical. |
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| 6. | | CAVALCANTE, J. M.; PEIXOTO, F. M.; BORGUINI, R. G.; PACHECO, S.; GOUVÊA, A. C. M. S.; SANTIAGO, M. C. P. de A.; GODOY, R. L. de O. Avaliação da bioacessibilidade de carotenoides em cenoura (Daucus carota). In: CONGRESSO LATINO AMERICANO DE CROMATOGRAFIA E TÉCNICAS RELACIONADAS, 14., 2012, Florianópolis. Livro de resumos. [S.l.: s.n.], 2012. p. 324.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| Registros recuperados : 6 | |
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