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Registro Completo |
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
11/08/2021 |
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Data da última atualização: |
11/08/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GOGONE, I. C. V. P.; FERREIRA, G. H.; GAVA, D.; SCHAEFER, R.; PAULA-LOPES, F. F. de; ROCHA, R. de A.; BARROS, F. R. O. de. |
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Afiliação: |
IZABEL C. V. P. COGONE, UTFPR; GLAUCIA H. FERREIRA, UNIFESP/Diadema; DANIELLE GAVA, CNPSA; REJANE SCHAEFER, CNPSA; FABÍOLA F. DE PAULA-LOPES, UNIFESP/Diadema; RAQUEL DE A. ROCHA, UTFPR; FLAVIA REGINA OLIVEIRA DE BARROS, UTFPR. |
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Título: |
Applicability of Raman spectroscopy on porcine parvovirus and porcine circovirus type 2 detection. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, v. 249, n. 119336, 2021. |
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DOI: |
https://doi.org/10.1016/j.saa.2020.119336 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Porcine parvovirus (PPV) is one of the major infectious causes of reproductive failure of swine. This disease is characterized by embryonic and fetal infection and death, responsible for important economic losses. PPV is also implicated as a trigger in the development of post-weaning multisystemic wasting syndrome (PMWS) caused by Porcine circovirus type 2 (PCV2). Their detection is PCR-based, which is quite sensitive and specific, but laborious, costly and time-demanding. Therefore, this study aimed to assess Raman spectroscopy (RS) as a diagnostic tool for PPV and PCV2 due to its label-free properties and unique ability to search and identify molecular fingerprints. Briefly, swine testis (ST) cells were inoculated with PPV or PCV2 and in vitro cultured (37 C, 5% CO2) for four days. Fixed cells were then submitted to RS investigation using a 633 nm laser. A total of 225 spectra centered at 1300 cm1 was obtained for each sample (5 spectra/cell; 15 cells/replicate; 3 replicates) of PPV-, PCV2-infected and uninfected (control) ST cells. Clear statistical discrimination between samples from both virus-infected cells was achieved with a Principal Component ? Linear Discriminant Analysis (PCA-LDA) model, reaching sensitivity rates from 95.55% to 97.77%, respectively to PCV2- and PPV-infected cells. These results were then submitted to a Leave-One-Out (LOO) validation algorithm resulting in 99.97% of accuracy. Extensive band assignment was analyzed and compiled for better understanding of PPV and PCV2 virus-cell interaction, demonstrating that specific protein, lipids and DNA/RNA bands are the most important assignments related to discrimination of virus-infected from uninfected cells. In conclusion, these results represent promising bases for RS application on PCV2 and PPV detection for future diagnostic applications. MenosAbstract: Porcine parvovirus (PPV) is one of the major infectious causes of reproductive failure of swine. This disease is characterized by embryonic and fetal infection and death, responsible for important economic losses. PPV is also implicated as a trigger in the development of post-weaning multisystemic wasting syndrome (PMWS) caused by Porcine circovirus type 2 (PCV2). Their detection is PCR-based, which is quite sensitive and specific, but laborious, costly and time-demanding. Therefore, this study aimed to assess Raman spectroscopy (RS) as a diagnostic tool for PPV and PCV2 due to its label-free properties and unique ability to search and identify molecular fingerprints. Briefly, swine testis (ST) cells were inoculated with PPV or PCV2 and in vitro cultured (37 C, 5% CO2) for four days. Fixed cells were then submitted to RS investigation using a 633 nm laser. A total of 225 spectra centered at 1300 cm1 was obtained for each sample (5 spectra/cell; 15 cells/replicate; 3 replicates) of PPV-, PCV2-infected and uninfected (control) ST cells. Clear statistical discrimination between samples from both virus-infected cells was achieved with a Principal Component ? Linear Discriminant Analysis (PCA-LDA) model, reaching sensitivity rates from 95.55% to 97.77%, respectively to PCV2- and PPV-infected cells. These results were then submitted to a Leave-One-Out (LOO) validation algorithm resulting in 99.97% of accuracy. Extensive band assignment was analyzed and compiled for bette... Mostrar Tudo |
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Palavras-Chave: |
Molecular fingerprint; PCV2; PPV; Reproductive failure; Viral diagnostic. |
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Thesagro: |
Diagnostico; Parvovirose; Sanidade Animal; Suíno. |
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Thesaurus Nal: |
Animal diseases; Porcine circovirus-2; Raman spectroscopy; Reproductive success; Swine. |
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Categoria do assunto: |
-- |
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Marc: |
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008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.saa.2020.119336$2DOI
100 1 $aGOGONE, I. C. V. P.
245 $aApplicability of Raman spectroscopy on porcine parvovirus and porcine circovirus type 2 detection.$h[electronic resource]
260 $c2021
520 $aAbstract: Porcine parvovirus (PPV) is one of the major infectious causes of reproductive failure of swine. This disease is characterized by embryonic and fetal infection and death, responsible for important economic losses. PPV is also implicated as a trigger in the development of post-weaning multisystemic wasting syndrome (PMWS) caused by Porcine circovirus type 2 (PCV2). Their detection is PCR-based, which is quite sensitive and specific, but laborious, costly and time-demanding. Therefore, this study aimed to assess Raman spectroscopy (RS) as a diagnostic tool for PPV and PCV2 due to its label-free properties and unique ability to search and identify molecular fingerprints. Briefly, swine testis (ST) cells were inoculated with PPV or PCV2 and in vitro cultured (37 C, 5% CO2) for four days. Fixed cells were then submitted to RS investigation using a 633 nm laser. A total of 225 spectra centered at 1300 cm1 was obtained for each sample (5 spectra/cell; 15 cells/replicate; 3 replicates) of PPV-, PCV2-infected and uninfected (control) ST cells. Clear statistical discrimination between samples from both virus-infected cells was achieved with a Principal Component ? Linear Discriminant Analysis (PCA-LDA) model, reaching sensitivity rates from 95.55% to 97.77%, respectively to PCV2- and PPV-infected cells. These results were then submitted to a Leave-One-Out (LOO) validation algorithm resulting in 99.97% of accuracy. Extensive band assignment was analyzed and compiled for better understanding of PPV and PCV2 virus-cell interaction, demonstrating that specific protein, lipids and DNA/RNA bands are the most important assignments related to discrimination of virus-infected from uninfected cells. In conclusion, these results represent promising bases for RS application on PCV2 and PPV detection for future diagnostic applications.
650 $aAnimal diseases
650 $aPorcine circovirus-2
650 $aRaman spectroscopy
650 $aReproductive success
650 $aSwine
650 $aDiagnostico
650 $aParvovirose
650 $aSanidade Animal
650 $aSuíno
653 $aMolecular fingerprint
653 $aPCV2
653 $aPPV
653 $aReproductive failure
653 $aViral diagnostic
700 1 $aFERREIRA, G. H.
700 1 $aGAVA, D.
700 1 $aSCHAEFER, R.
700 1 $aPAULA-LOPES, F. F. de
700 1 $aROCHA, R. de A.
700 1 $aBARROS, F. R. O. de
773 $tSpectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy$gv. 249, n. 119336, 2021.
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Registro original: |
Embrapa Suínos e Aves (CNPSA) |
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| Registros recuperados : 149 | |
| 81. |  | MORI, A. P.; PERETTI, R. C.; FERRAZ, R.; FERRAZ, G. M.; GAVA, D.; HAACH, V.; SCHAEFER, R. Occurrence of influenza A virus subtypes in Brazil. In: ALLEN D. LEMAN SWINE CONFERENCE, 2020, Saint Paul. Research Abstract. Saint Paul: University of Minnesota/College of Veterinary Medicine, 2020. Poster number: 70.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 82. | | SCHAEFER, R.; GAVA, D.; CANTAO, M. E.; SERÃO, V. H. B.; SILVA, M. C.; MORES, N.; ZANELLA, J. R. C. PCV2 disease in vaccinated growing pigs in Southern Brazil. In: INTERNATIONAL PIG VETERINARY SOCIETY CONGRESS, 23., Cancun. Proceedings... León, México: IPVS, 2014. p. 522| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 83. | | ZANELLA, J. R. C.; GAVA, D.; SCHAEFER, R.; COLDEBELLA, A.; SILVEIRA, S.; SIMON, N. L.; SCHIOCHET, M. F.; SCHWARZ, P. Perfil da infecção por vírus de influenza em suínos no Brasil após a introdução do vírus pandêmico H1N1 em 2009. In: CONGRESSO BRASILEIRO DE VETERINÁRIOS ESPECIALISTAS EM SUÍNOS, 16., 2013, Cuiabá. Anais... Guiabá: Abraves; UFMT, 2013. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 84. | | MORES, N.; CARON, L.; MORES, M. A. Z.; GAVA, D.; ESTEVES, P. A.; ZANELLA, J. R. C. Mumificação fecal associado ao circovírus suíno tipo 2 (PCV2) em um rebanho livre de patógenos específicos (SPF). In: SIMPÓSIO INTERNACIONAL DE SUINOCULTURA, 7., Porto Alegre, RS. Anais... Porto Alegre: UFRGS, 2012. p. 283-286. Projeto: 03.07.05.019. SINSUI.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 85. | | GAVA, D.; SCHAEFER, R.; SERRÃO, V. H. B.; CANTAO, M. E.; SILVA, M. C.; ZANELLA, J. R. C. Mutations in the capsid protein of porcine circovirus type 2 indicates a viral immune evasion. In: Journal of the Brazilian Society for Virology, Ribeirão Preto, v. 19, p. 234-235, 2014. Suppl. 2. Edição dos anais do 25º. Brazilian Congress of Virology e 9º. Mercosur Meeting of Virology, Ribeirão Preto, set./out, 2014.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 86. | | GAVA, D.; SOUZA, C. K.; SCHAEFER, R.; VINCENT, A. L.; CANTAO, M. E.; COLDEBELLA, A.; ZANELLA, J. R. C. A TaqMan-based real-time PCR for detection and quantification of porcine parvovirus 4. Journal of Virological Methods, v. 219, p. 14-17, 2015.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 87. | | SOUZA, T. C. G. D. de; GAVA, D.; SCHAEFER, R.; LEME, R. A.; PORTO, G. da S.; ALFIERI, A. A. Porcine circovirus 3a field strains in free-livingwild boars in Paraná state, Brazil. Animals, v. 11, n. 1634, 2021.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 88. | | SOUZA, T. C. G. D. de; GAVA, D.; LEME, R. A.; SCHAEFER, R.; PORTO, G. S.; ALFIERI, A. A. Porcine circovirus type 3 in free-living wild boars in Brazil. In: INTERNATIONAL PIG VETERINARY SOCIETY CONGRESS, 26., 2020, Rio de Janeiro. Proceedings... Rio de Janeiro, RJ: IPVS, 2020. p. 757.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 89. | | RECH, R. R.; GAVA, D.; SILVA, M. C. da; FERNANDES, L. T.; HAACH, V.; ZANELLA, J. R. C.; SCHAEFER, R. Porcine respiratory disease complex after the introduction of H1N1/2009 influenza virus in Brazil. Zoonoses and Public Health, v. 65, n.1, p. e155-e161, 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 90. | | SILVA, M. C.; RECH, R. R.; GAVA, D.; KUCHIISHI, S. S.; SCHAEFER, R.; LANGOHR, I. M.; ZANELLA, J. R. C. Porcine respiratory disease complex in southern Brazil: pathogens and associated histopathology. In: CONCURRENT ANNUAL METING, 2013, Montreál. Abstract detail. Montreál: American College of Veterinary Pathologists, 2013. Poster D-32.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 91. | | GAVA, D.; SERRÃO, V. H. B.; FERNANDES, L. T.; CANTAO, M. E.; ZANELLA, J. R. C.; MORES, N.; SCHAEFER, R. Structure analysis of capsid protein of porcine circovirus type 2 from pigs with systemic disease. Brazilian Journal of Microbiology, v. 49, p. 351-357, 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 92. | | ZANELLA, J. R. C.; GAVA, D.; SCHAEFER, R.; CARON, L.; SILVA, V. S.; ARAÚJO JÚNIOR, J. P.; CRUZ, T. F.; ZIMMERMANN, S.; ZIMMERMANN, J. J. Absence of PRRSV antibodies and antigen in sera and oral fluids samples from Brazilian swine herds. In: BRAZILIAN CONGRESS OF VIROLOGY, 23.; MERCOSUR MEETING OF VIROLOGY, 7., 2012, Foz do Iguaçu. Virus and Research? Foz do Iguaçu: SBV, 2012. p.424. Journal of the Brazilian Society for Virology, v. 17, n. 2, suppl. 1, 2012. Projeto/Plano de Ação: 02.11.01.006.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 93. | | GOGONE, I. C. V. P.; FERREIRA, G. H.; GAVA, D.; SCHAEFER, R.; PAULA-LOPES, F. F. de; ROCHA, R. de A.; BARROS, F. R. O. de. Applicability of Raman spectroscopy on porcine parvovirus and porcine circovirus type 2 detection. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, v. 249, n. 119336, 2021.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 94. | | GAVA, D.; ARGENTI, L. E.; MORES, T. J.; SOUZA, C. K.; STRECK, A. F.; BERNARDI, M. L.; CANAL, C. W.; BORTOLOZZO, F. P.; WENTZ, I. Caracterização do perfil sorológico de nulíparas suínas e da progênie frente ao parvovírus suíno. In: CONGRESSO BRASILEIRO DE VETERINÁRIOS ESPECIALISTAS EM SUÍNOS, 15., 2011, Fortaleza. Anais de palestras e resumos. Fortaleza: Abraves, 2011. 1 CD-ROM. Projeto: 03.09.00.046.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 95. | | MACIAG, S. S.; BELLAVER, F. A. V.; FONSECA, F. M. da; BOMBASSARO, G. E.; GAVA, D.; LOPES, L. dos S.; BASTOS, A. P. A. Comparison of cell viability in fresh and frozen porcine colostrums. In: INTERNATIONAL PIG VETERINARY SOCIETY CONGRESS, 26., 2020, Rio de Janeiro. Proceedings... Rio de Janeiro, RJ: IPVS, 2020. p. 561.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 96. | | BORDIN, L. C.; GAVA, D.; SIMON, N. L.; ROCHA, C. S.; ESTEVES, P. A.; MORES, N.; COLDEBELLA, A.; ZANELLA, J. R. C.; VAZ, E. K. Comparison of conventional and real time PCR to detect Mycoplasma suis in Brazilian herds with reproductive disorders. In: INTERNATIONAL PIG VETERINARY SOCIETY CONGRESS, 22., 2012, Jeju. Proceedings... Jeju: IPVS, 2012. p. 689. Projeto/Plano de Ação: 02.11.01.006.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 97. | | BOMBASSARO, G. E.; BELLAVER, F. A. V.; FONSECA, F. M. da; MACIAG, S. S.; GAVA, D.; LOPES, L. dos S.; BASTOS, A. P. A. Comparison of immune cells of colostrum from gilts and sows. In: INTERNATIONAL PIG VETERINARY SOCIETY CONGRESS, 26., 2020, Rio de Janeiro. Proceedings... Rio de Janeiro, RJ: IPVS, 2020. p. 562.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 98. | | HAACH, V.; BASTOS, A. P. A.; GAVA, D.; FONSECA, F. M. da; MORES, M. A. Z.; COLDEBELLA, A.; FRANCO, A. C.; SCHAEFER, R. Cellular and humoral immunity elicited by an Influenza A polyvalent virosomal vaccine in pigs. In: INTERNATIONAL PIG VETERINARY SOCIETY CONGRESS, 26., 2022, Rio de Janeiro. Proceedings... Rio de Janeiro, RJ: IPVS: Abraves, 2022. p. 421. In: INTERNATIONAL PIG VETERINARY SOCIETY CONGRESS, 26., 2022, Rio de Janeiro. Proceedings... Rio de Janeiro, RJ: IPVS: Abraves, 2022. p. 421.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 99. | | BIONDO, N.; GAVA, D.; SIMON, N. L.; ROCHA, C. S.; SILVEIRA, S.; SCHIOCHET, M. F.; MORES, M. A. Z.; SCHAEFER, R.; ZANELLA, J. R. C. First detection and caracterization of pandemic H1N1 influenza virus and association with Mycoplasma hyopneumoniae in captive wild boar in Brazil. In: INTERNATIONAL PIG VETERINARY SOCIETY CONGRESS, 22., 2012, Jeju. Proceedings... Jeju: IPVS, 2012. p. 413. Projeto/Plano de Ação: 02.11.01.006.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 100. | | SCHAEFER, R.; LOPES, L. dos S.; GAVA, D.; ZANELLA, J. R. C.; ANDERSON, T. K.; LEWIS, N. S.; VINCENT, A. L. Genetic and antigenic diversity of contemporary influenza A virus in swine in Brazil. In: INTERNATIONAL PIG VETERINARY SOCIETY CONGRESS, 26., 2020, Rio de Janeiro. Proceedings... Rio de Janeiro, RJ: IPVS, 2020. p. 828.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| Registros recuperados : 149 | |
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