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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
01/11/2019 |
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Data da última atualização: |
01/11/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
HAACH, V.; GAVA, D.; CANTAO, M. E.; FRANCO, A. C. |
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Afiliação: |
VANESSA HAACH, UFRGS; DANIELLE GAVA, CNPSA; MAURICIO EGIDIO CANTAO, CNPSA; ANA CLÁUDIA FRANCO, UFRGS. |
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Título: |
One-step multiplex RT-qPCR for the detection and subtyping of influenza A virus in swine in Brazil. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Journal of Virological Methods, v. 269, p. 43-48, 2019. |
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DOI: |
10.1016/j.jviromet.2019.04.005 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Pandemic H1N1, human-like H1N2 and H3N2 influenza A (IAV) viruses are co-circulating in swine herds in Brazil. The genetic analysis of the Brazilian IAVs has shown that they are genetically distinct from viruses found in swine in other countries; therefore, an update of the diagnostic assays for IAV detection and subtyping is needed. This study describes the development and validation of a TaqMan based ? one-step multiplex RT-qPCR to discriminate the hemagglutinin and neuraminidase genes of the three major IAV ubtypes circulating in pigs in Brazil. The RT-qPCR assays presented 100% (95.7?100, CI 95%) of diagnostic sensitivity in the analysis of 85 IAVs, previously characterized by sequencing. The limits of detection ranged from 5.09 × 101 to 5.09 × 103 viral RNA copies/μL. For the analytical specificity, 73 pig samples collected during 2017 and 2018 were analyzed, resulting in the identification of the subtype in 74.0% (62.9?82.7, CI 95%) of samples. From these, 46.3% were H3N2, 33.3% were H1N1, 11.1% were H1N2 and 3.7% were HxN1. Mixed viral infections (3.7%) and reassortant viruses (1.9%) were also detected by the test. This multiplex RT-qPCR assay provides a fast and specific diagnostic tool for identification of different subtypes and lineages of IAV in pigs, contributing to the monitoring of influenza in swine. |
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Palavras-Chave: |
Diagnóstico rápido; Multiplex RT-qPCR; Rapid diagnosis; RT-qPCR multiplex; Subtipagem; Subtyping; Vírus da gripe A. |
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Thesagro: |
Suíno. |
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Thesaurus Nal: |
Influenza A virus; Swine. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02191naa a2200289 a 4500
001 2113827
005 2019-11-01
008 2019 bl uuuu u00u1 u #d
024 7 $a10.1016/j.jviromet.2019.04.005$2DOI
100 1 $aHAACH, V.
245 $aOne-step multiplex RT-qPCR for the detection and subtyping of influenza A virus in swine in Brazil.$h[electronic resource]
260 $c2019
520 $aAbstract: Pandemic H1N1, human-like H1N2 and H3N2 influenza A (IAV) viruses are co-circulating in swine herds in Brazil. The genetic analysis of the Brazilian IAVs has shown that they are genetically distinct from viruses found in swine in other countries; therefore, an update of the diagnostic assays for IAV detection and subtyping is needed. This study describes the development and validation of a TaqMan based ? one-step multiplex RT-qPCR to discriminate the hemagglutinin and neuraminidase genes of the three major IAV ubtypes circulating in pigs in Brazil. The RT-qPCR assays presented 100% (95.7?100, CI 95%) of diagnostic sensitivity in the analysis of 85 IAVs, previously characterized by sequencing. The limits of detection ranged from 5.09 × 101 to 5.09 × 103 viral RNA copies/μL. For the analytical specificity, 73 pig samples collected during 2017 and 2018 were analyzed, resulting in the identification of the subtype in 74.0% (62.9?82.7, CI 95%) of samples. From these, 46.3% were H3N2, 33.3% were H1N1, 11.1% were H1N2 and 3.7% were HxN1. Mixed viral infections (3.7%) and reassortant viruses (1.9%) were also detected by the test. This multiplex RT-qPCR assay provides a fast and specific diagnostic tool for identification of different subtypes and lineages of IAV in pigs, contributing to the monitoring of influenza in swine.
650 $aInfluenza A virus
650 $aSwine
650 $aSuíno
653 $aDiagnóstico rápido
653 $aMultiplex RT-qPCR
653 $aRapid diagnosis
653 $aRT-qPCR multiplex
653 $aSubtipagem
653 $aSubtyping
653 $aVírus da gripe A
700 1 $aGAVA, D.
700 1 $aCANTAO, M. E.
700 1 $aFRANCO, A. C.
773 $tJournal of Virological Methods$gv. 269, p. 43-48, 2019.
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| 1. |  | SARRIA, A. L. F.; PEREIRA, J. F.; PEREIRA, P. R. V. da S.; ARADOTTIR, G. I.; MARTIN, J.; SMART, L.; PICKETT, J. A.; BIRKETT, M. A. Aphid resistance in brazilian hexaploid wheat does not correlate with levels of the benzoxazinoid natural product DIMBOA. In: JOINT MEETING, 1., 2016, Iguassu Falls. Abstracts... [Iguassu Falls]: International Society of Chemical Ecology: Latin American Association of Chemical Ecology, 2016. p. 80. Evento conjunto - 32nd Annual Meeting of the International Society of Chemical Ecology e 4th Congress of the Latin American Association of Chemical Ecology.| Tipo: Resumo em Anais de Congresso |
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| 2. | | MORAES, M. C. B.; BIRKETT, M. A.; GORDON WEEKS, R.; SMART, L. E.; MARTIN, J. L.; PYE, B. J.; BROMILOW, R.; PICKETT, J. A. Cis-jasmone induces accumulation of defence compounds in wheat, Triticum aestivum. Phytochemistry, v. 69, p. 9-17, 2008.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 3. | | PEREIRA, J. F.; SARRIA, A. L. F.; POWERS, S. J.; ARADOTTIR, G. I.; CAULFIELD, J. C.; MARTIN, J.; SMART, L. E.; PICKETT, J. A.; BIRKETT, M. A.; PEREIRA, P. R. V. da S. DIMBOA levels in hexaploid Brazilian wheat are not associated with antibiosis against the cereal aphids Rhopalosiphum padi and Sitobion avenae. Theoretical and Experimental Plant Physiology, Campo dos Goytacazes, v. 29, n. 2, p. 61-75, June 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Gado de Leite; Embrapa Trigo. |
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| 4. | | TUSKAN, G. A.; GROOVER, A. T.; SCHMUTZ, J.; DIFAZIO, S. P.; MYBURG, A.; GRATTAPAGLIA, D.; SMART, L. B.; YIN, T.; AURY, J.-M.; KREMER, A.; LEROY, T.; LE PROVOST, G.; PLOMION, C.; CARLSON, J. E.; RANDALL, J.; WESTBROOK, J.; GRIMWOOD, J.; MUCHERO, W.; JACOBSON, D.; MICHENER, J. K. Hardwood tree genomics: unlocking woody plant biology. Frontiers in Plant Science, v. 9, article 1799, 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
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