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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
04/10/2001 |
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Data da última atualização: |
04/10/2001 |
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Autoria: |
GARCIA DE SALAMONE, I. E.; HYNES, R. K.; NELSON, L. M. |
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Título: |
Cytokinin production by plant growth promoting rhizobacteria and slected mutants. |
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Ano de publicação: |
2001 |
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Fonte/Imprenta: |
Canadian Journal of Microbiology, Ottawa, v. 47, p. 404 - 411, |
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Idioma: |
Inglês |
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Conteúdo: |
One the proposed mechanisms by which rhizobacteria enhace plant growth is through the production of plant growth regulators. Five plant growth promoting rhizobacterial (PGPR) strains produced the cytokinin dihydrozeatin ribiside (DHZR) in pure culture. Cytokinin production by fluorescens G20 - 18, a rifampicin - resistant mutant (RIF), and two TnphoA-derived mutants (CNT1, CNT2), with reduced capacity to synthesize cytokinins, was further characterized in pure culture using immunoassay and thin layer chromatography. G20 - 18 produced higher amounts of three cytokinins, isopentenyl adenosine (IPA), trans-zeatin ribose (ZR), and DHZR than the three mutants during stationary phase. IPA was the major metabolite produced, but the proportion of ZR and DHZR accumulated by CNT1 and CNT2 increased with time. No differences were observed between strain G20 - 18 and the mutants in the amounts of indole acetic acid synthesized, nor were gibberellins detected in supernatants of any of the strains. Addition of 10-5 M adenine increased cytokinin production in 96 - and 168-h cultures of strain G20 - 18 by approximately 67%. G20 -18 and the mutants CNT1 and CNT2 may be useful for determination of the role of cytokinin production by PGPR. |
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Palavras-Chave: |
Chromatograph; Espectometro de massa; Growth; PGPR; Plats. |
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Thesagro: |
Bactéria; Crescimento; Cromatografia; Microrganismo; Planta; Pseudomonas Fluorescens; Rizosfera; Solo. |
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Thesaurus Nal: |
mass spectrometry; microorganisms; rhizosphere; soil. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02150naa a2200349 a 4500
001 1621264
005 2001-10-04
008 2001 bl --- 0-- u #d
100 1 $aGARCIA DE SALAMONE, I. E.
245 $aCytokinin production by plant growth promoting rhizobacteria and slected mutants.
260 $c2001
520 $aOne the proposed mechanisms by which rhizobacteria enhace plant growth is through the production of plant growth regulators. Five plant growth promoting rhizobacterial (PGPR) strains produced the cytokinin dihydrozeatin ribiside (DHZR) in pure culture. Cytokinin production by fluorescens G20 - 18, a rifampicin - resistant mutant (RIF), and two TnphoA-derived mutants (CNT1, CNT2), with reduced capacity to synthesize cytokinins, was further characterized in pure culture using immunoassay and thin layer chromatography. G20 - 18 produced higher amounts of three cytokinins, isopentenyl adenosine (IPA), trans-zeatin ribose (ZR), and DHZR than the three mutants during stationary phase. IPA was the major metabolite produced, but the proportion of ZR and DHZR accumulated by CNT1 and CNT2 increased with time. No differences were observed between strain G20 - 18 and the mutants in the amounts of indole acetic acid synthesized, nor were gibberellins detected in supernatants of any of the strains. Addition of 10-5 M adenine increased cytokinin production in 96 - and 168-h cultures of strain G20 - 18 by approximately 67%. G20 -18 and the mutants CNT1 and CNT2 may be useful for determination of the role of cytokinin production by PGPR.
650 $amass spectrometry
650 $amicroorganisms
650 $arhizosphere
650 $asoil
650 $aBactéria
650 $aCrescimento
650 $aCromatografia
650 $aMicrorganismo
650 $aPlanta
650 $aPseudomonas Fluorescens
650 $aRizosfera
650 $aSolo
653 $aChromatograph
653 $aEspectometro de massa
653 $aGrowth
653 $aPGPR
653 $aPlats
700 1 $aHYNES, R. K.
700 1 $aNELSON, L. M.
773 $tCanadian Journal of Microbiology, Ottawa$gv. 47, p. 404 - 411
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Embrapa Agrobiologia (CNPAB) |
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