| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Agricultura Digital; Embrapa Gado de Corte. |
|
Data corrente: |
28/01/2020 |
|
Data da última atualização: |
06/11/2020 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
GIACHETTO, P. F.; CUNHA, R. C.; NHANI JUNIOR, A.; GARCIA, M. V.; FERRO, J. A.; ANDREOTTI, R. |
|
Afiliação: |
POLIANA FERNANDA GIACHETTO, CNPTIA; RODRIGO CASQUERO CUNHA, UFPEL; ANTONIO NHANI JUNIOR, CNPTIA; MARCOS VALERIO GARCIA, CNPGC; JESUS APARECIDO FERRO, UNESP; RENATO ANDREOTTI E SILVA, CNPGC. |
|
Título: |
Gene expression in the salivary gland of Rhipicephalus (Boophilus) microplus fed on tick-susceptible and tick-resistant hosts. |
|
Ano de publicação: |
2020 |
|
Fonte/Imprenta: |
Frontiers in Cellular and Infection Microbiology, v. 9, p. 1-15, Jan. 2020. |
|
DOI: |
10.3389/fcimb.2019.0047 |
|
Idioma: |
Inglês |
|
Notas: |
Article 477. |
|
Conteúdo: |
The success of cattle tick fixation largely depends on the secretion of substances that alter the immune response of the host. The majority of these substances are expressed by the parasite salivary gland and secreted in tick saliva. It is known that hosts can mount immune responses against ticks and bovine European breeds, and bovine industrial crossbreeds are more susceptible to infestations than are Bos indicus cattle. To identify candidates for the development of novel control strategies for the cattle tick Rhipicephalus (Boophilus) microplus, a salivary gland transcriptome analysis of engorged females fed on susceptible or resistant hosts was performed. Using RNA-Seq, transcriptomes were de novo assembled and produced a total of 235,451 contigs with 93.3% transcriptome completeness. Differential expression analysis identified 137 sequences as differentially expressed genes (DEGs) between ticks raised on tick-susceptible or tick-resistant cattle. DEGs predicted to be secreted proteins include innexins, which are transmembrane proteins that form gap junction channels; the transporters Na+/dicarboxylate, Na+/tricarboxylate, and phosphate transporter and a putative monocarboxylate transporter; a phosphoinositol 4-phosphate adaptor protein; a cysteine-rich protein containing a trypsin inhibitor-like (TIL) domain; a putative defense protein 3 containing a reeler domain; and an F-actin-uncapping protein LRRC16A with a CARMIL_C domain; these genes were upregulated in ticks fed on tick-susceptible cattle. DEGs predicted to be non-secreted proteins included a small heat shock protein and the negative elongation factor B-like, both acting in a coordinated manner to increase HSP transcript levels in the salivary glands of the ticks fed on tick-susceptible cattle; the 26S protease regulatory subunit 6B and another chaperone with similarity to calnexin, also upregulated in ticks fed on tick-susceptible cattle; an EF-hand calcium binding protein and a serine carboxypeptidase (SCP), both involved in the blood coagulation cascade and upregulated in ticks fed on tick-susceptible cattle; and two ribosomal proteins, the 60S acidic ribosomal protein P2 and the 60S ribosomal protein L19. These results help to characterize cattle tick salivary gland gene expression in tick-susceptible and tick-resistant hosts and suggest new putative targets for the control of tick infestations, as those genes involved in the mechanism of stress response during blood feeding. MenosThe success of cattle tick fixation largely depends on the secretion of substances that alter the immune response of the host. The majority of these substances are expressed by the parasite salivary gland and secreted in tick saliva. It is known that hosts can mount immune responses against ticks and bovine European breeds, and bovine industrial crossbreeds are more susceptible to infestations than are Bos indicus cattle. To identify candidates for the development of novel control strategies for the cattle tick Rhipicephalus (Boophilus) microplus, a salivary gland transcriptome analysis of engorged females fed on susceptible or resistant hosts was performed. Using RNA-Seq, transcriptomes were de novo assembled and produced a total of 235,451 contigs with 93.3% transcriptome completeness. Differential expression analysis identified 137 sequences as differentially expressed genes (DEGs) between ticks raised on tick-susceptible or tick-resistant cattle. DEGs predicted to be secreted proteins include innexins, which are transmembrane proteins that form gap junction channels; the transporters Na+/dicarboxylate, Na+/tricarboxylate, and phosphate transporter and a putative monocarboxylate transporter; a phosphoinositol 4-phosphate adaptor protein; a cysteine-rich protein containing a trypsin inhibitor-like (TIL) domain; a putative defense protein 3 containing a reeler domain; and an F-actin-uncapping protein LRRC16A with a CARMIL_C domain; these genes were upregulated in ticks fed ... Mostrar Tudo |
|
Palavras-Chave: |
Bioinformática; Cattle tick; Expressão gênica; Host-parasite interaction; RNA-Seq. |
|
Thesagro: |
Carrapato; Gado de Corte. |
|
Thesaurus Nal: |
Bioinformatics; Cattle; Rhipicephalus microplus; Sialome; Transcriptome. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/215164/1/AP-Gene-expression-in-the-salivary-2020.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/209727/1/Gene-expression-in-the-salivary.pdf
|
|
Marc: |
LEADER 03526naa a2200349 a 4500
001 2124241
005 2020-11-06
008 2020 bl uuuu u00u1 u #d
024 7 $a10.3389/fcimb.2019.0047$2DOI
100 1 $aGIACHETTO, P. F.
245 $aGene expression in the salivary gland of Rhipicephalus (Boophilus) microplus fed on tick-susceptible and tick-resistant hosts.$h[electronic resource]
260 $c2020
500 $aArticle 477.
520 $aThe success of cattle tick fixation largely depends on the secretion of substances that alter the immune response of the host. The majority of these substances are expressed by the parasite salivary gland and secreted in tick saliva. It is known that hosts can mount immune responses against ticks and bovine European breeds, and bovine industrial crossbreeds are more susceptible to infestations than are Bos indicus cattle. To identify candidates for the development of novel control strategies for the cattle tick Rhipicephalus (Boophilus) microplus, a salivary gland transcriptome analysis of engorged females fed on susceptible or resistant hosts was performed. Using RNA-Seq, transcriptomes were de novo assembled and produced a total of 235,451 contigs with 93.3% transcriptome completeness. Differential expression analysis identified 137 sequences as differentially expressed genes (DEGs) between ticks raised on tick-susceptible or tick-resistant cattle. DEGs predicted to be secreted proteins include innexins, which are transmembrane proteins that form gap junction channels; the transporters Na+/dicarboxylate, Na+/tricarboxylate, and phosphate transporter and a putative monocarboxylate transporter; a phosphoinositol 4-phosphate adaptor protein; a cysteine-rich protein containing a trypsin inhibitor-like (TIL) domain; a putative defense protein 3 containing a reeler domain; and an F-actin-uncapping protein LRRC16A with a CARMIL_C domain; these genes were upregulated in ticks fed on tick-susceptible cattle. DEGs predicted to be non-secreted proteins included a small heat shock protein and the negative elongation factor B-like, both acting in a coordinated manner to increase HSP transcript levels in the salivary glands of the ticks fed on tick-susceptible cattle; the 26S protease regulatory subunit 6B and another chaperone with similarity to calnexin, also upregulated in ticks fed on tick-susceptible cattle; an EF-hand calcium binding protein and a serine carboxypeptidase (SCP), both involved in the blood coagulation cascade and upregulated in ticks fed on tick-susceptible cattle; and two ribosomal proteins, the 60S acidic ribosomal protein P2 and the 60S ribosomal protein L19. These results help to characterize cattle tick salivary gland gene expression in tick-susceptible and tick-resistant hosts and suggest new putative targets for the control of tick infestations, as those genes involved in the mechanism of stress response during blood feeding.
650 $aBioinformatics
650 $aCattle
650 $aRhipicephalus microplus
650 $aSialome
650 $aTranscriptome
650 $aCarrapato
650 $aGado de Corte
653 $aBioinformática
653 $aCattle tick
653 $aExpressão gênica
653 $aHost-parasite interaction
653 $aRNA-Seq
700 1 $aCUNHA, R. C.
700 1 $aNHANI JUNIOR, A.
700 1 $aGARCIA, M. V.
700 1 $aFERRO, J. A.
700 1 $aANDREOTTI, R.
773 $tFrontiers in Cellular and Infection Microbiology$gv. 9, p. 1-15, Jan. 2020.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Agricultura Digital (CNPTIA) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 272 | |
| 162. |  | CSORDAS, B. G.; ALMEIDA, R. F. C. de; COMINETTI, M. C.; CUNHA, R. C.; GARCIA, M. V.; ANDREOTTI, R. Coatis (Nasua nasua) as reservoirs and amplifiers pathogens transmitted by ticks. In: INTERNATIONAL SYMPOSIUM ON TICK CONTROL AND TICK-BORNE DISEASES, 2013, Campo Grande, MS. [Proceedings..]. Campo Grande, MS: Embrapa Gado de Corte, 2013. (Embrapa Gado de Corte. Documentos, 202). Comissão organizadora: Renato Andreotti, Fernando Paiva, Wilson Werner Koller, Jacqueline Cavalcante Barros, Luiz Antonio Dias Leal, Jaqueline Matias.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Corte. |
|    |
| 163. | | GONÇALVES, G. S.; DAMASCENO, L. A.; SILVA, J. F.; GARCIA, M. V. B.; CAVALCANTE, A. M. L. N. Controle de erva-de-passarinho em citros com herbicidas. In: CONGRESSO BRASILEIRO DA CIÊNCIA DAS PLANTAS DANINHAS, 28., 2012, Campo Grande, MS. A Ciência das plantas daninhas na era da biotecnologia. Palestras. Campo Grande, MS: Sociedade Brasileira da Ciência das Plantas Daninhas, 2012. p. 193-197.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
| |
| 165. | | MONTEIRO, G. F. P.; SILVA, J. F.; BORGES, J. E. C.; GARCIA, M. V. B.; GARCIA, T. B. Período crítico de interferência de plantas daninhas na cultura dos citros no Município de Manaus. In: CONGRESSO BRASILEIRO DA CIÊNCIA DAS PLANTAS DANINHAS, 28., 2012, Campo Grande, MS. A Ciência das plantas daninhas na era da biotecnologia. Palestras. Campo Grande, MS: Sociedade Brasileira da Ciência das Plantas Daninhas, 2012. p. 17-21.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
| |
| 166. | | KANO, C.; GARCIA, T. B.; ARRUDA, M. R. de; GARCIA, M. V. B.; AZEVEDO, C. L. L.; MARTINS, G. C. Crescimento, produção de massa seca e acúmulo de nutrientes em porta-enxertos cítricos cultivados em Manaus, Amazonas. Manaus: Embrapa Amazônia Ocidental, 2018. 18 p. (Embrapa Amazônia Ocidental. Boletim de Pesquisa e Desenvolvimento, 26).| Tipo: Boletim de Pesquisa e Desenvolvimento |
| Biblioteca(s): Embrapa Amazônia Ocidental; Embrapa Territorial. |
| |
| 167. | | RABELING, C.; GONZALES, O.; SCHULTZ, T. R.; BACCI JR. M.; GARCIA, M. V. B.; VERHAAGH, M.; ISHAK, H. D. Cryptic sexual populations account for genetic diversity and ecological success in a widely distributed, asexual fungus-growing ant. Proceedings of the National Academy of Sciences, v. 108, n. 30, p. 12366-12371, Jul., 2011.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
|  |
| 168. | | GARCIA, M. V.; MATIAS, J.; BARROS, J. C.; LIMA, D. P. de; LOPES, R. da S.; ANDREOTTI, R. Chemical identification of Tagetes minuta Linnaeus (Asteraceae) essential oil and its acaricidal effect on ticks. Revista Brasileira de Parasitologia Veterinária, Jaboticabal, v. 21, n. 4, p. 57-63, out.-dez. 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Gado de Corte. |
| |
| 170. | | RICO, A.; GEBER-CORRÊA, R.; CAMPOS, P. S.; GARCIA, M. V. B.; WAICHMAN, A. V.; BRINK, P. J. van den. Effect of Parathion-Methyl on Amazonian fish and freshwater invertebrates: a comparison of sensitivity with temperate data. Archives of Environmental Contamination and Toxicology, v. 58, n. 3, p. 765-771, 2010.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
| |
| 171. | | ALMEIDA, R. F. C.; GARCIA, M. V.; CUNHA, R. C.; MATIAS, J; LABRUNA, M. B.; ANDREOTTI, R. The first report of Rickettsia spp. in Amblyomma nodosum in the State of Mato Grosso do Sul, Brazil. Ticks and Tick Borne Diseases, v. 4, n.1-2, p. 156-159,Feb. 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Gado de Corte. |
| |
| 172. | | ALMEIDA, R. F. C. de; GARCIA, M. V.; MATIAS, J.; MATOS, M. de F. C.; ANDREOTTI, R. Epidemiologia das riquétsia do grupo da febre maculosa brasileira no Estado de Mato Grosso do Sul. In: CONGRESSO BRASILEIRO DE PARASITOLOGIA VETERINÁRIA, 17., 2012, São Luis. Parasitologia veterinária, bem estar e produção animal: anais. São Luis: Colégio Brasileiro de Parasitologia Veterinária, 2012. p.264 PPR 253| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Corte. |
| |
| 173. | | CSORDAS, B. G.; ALMEIDA, R. F. C. de; COMINETTI, M. C.; CUNHA, R. C.; GARCIA, M. V.; ANDREOTTI, R. Quatis (nasua nasua) como reservatórios e amplificadores de patógenos transmitidos por carrapatos. In: CONGRESSO DA SOCIEDADE BRASILEIRA DE MEDICINA TROPICAL, 49., 2013, Campo Grande, MS. Anais... Campo Grande: SBMT, 2013.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Corte. |
| |
| 174. | | MARTIUS, C.; HOEFER, H.; BECK, L.; GARCIA, M. V. B.; LUIZAO, F.; FRANKLIN, E.; MORAIS, J. W. de; FORSTER, B. A quantitative model of the role of soil fauna in decomposition as affected by different forested cropping systems in central Amazonia. In: GERMAN-BRAZILIAN WORKSHOP ON NEOTROPICAL ECOSYSTEMS, 2000, Hamburg. Programa and abstracts... Hamburg: University, 2000. p. 108.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
| |
| 175. | | MARTIUS, C.; HOEFER, H.; BECK, L.; GARCIA, M. V. B.; LUIZÃO, F.; FRANKLIN, E.; MORAIS, J. W. de; FÖRSTER, B. A quantitative model of the role of soil fauna in decomposition as affected by different forested cropping systems in Central Amazonia. In: GERMAN-BRAZILIAN WORKSHOP ON NEOTROPICAL ECOSYSTEMS. ACHIEVEMENTS AND PROSPECTS OF COOPERATIVE RESEARCH, 2000, Hamburg. Proceedings... Hamburg: SHIFT: MADAM: WAVES, 2002. p. 204.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
| |
| 180. | | KUPERMAN, R. G.; CHECKAI, R. T.; GARCIA, M. V. B.; RÖMBKE, J.; STEPHENSON, G. L.; SOUSA, J. P. State of the science and the way forward for the ecotoxicological assessment of contaminated land. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 44, n. 8, p. 811-824, ago. 2009.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Amazônia Ocidental; Embrapa Unidades Centrais. |
| |
| Registros recuperados : 272 | |
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
