Data/hora: 15/06/2024 18:12:44

Biblioteca(s): 	Embrapa Mandioca e Fruticultura.	
Data corrente: 	05/10/1993	
Data da última atualização: 	05/10/1993	
Autoria: 	GARCIA, M. L.; ARRESE, E. L.; GRAU, O.; SARACHU, A. N.	
Afiliação: 	Catedra de Quimica Biologica II, Facultad de Ciencias Exactas, U. N.,La Plata 47 y 115, 1900 La Plata, Argentina.	
Título: 	Citrus Psorosic Disease Agent Behaves as a two Componet ss RNA Virus.	
Ano de publicação: 	1991	
Fonte/Imprenta: 	Riverside, California: International Organization of Citrus Virologists, 1991.	
Páginas: 	337-344	
Idioma: 	Inglês	
Notas: 	Conference of the International Organization of Citrus Virologists, 11., 1991, Riverside, California, Proceedings...	
Conteúdo: 	Citrus seedings were graft-inoculated with psorosis (isolate 90-1-1;Concordia, Argentina).When symptomatic young leaves were ground, homogeniized and centrifuged at low speed, infectivity (local lesions on Chenopodium quinoa) remained in the supernatant.Polyethylene glycol precipitation and high-speed centrifugation yielded infective pellets. Infectivity was abolished by RNase treatment, even in 0.3 M NaCI,indicating that ss RNA (s) is essential for biological activity. Protein(s) is also required for infectivity, since it was sensitivy to phenol extraction and proteinase K treatment. When high speed pellts were subjected to sucrose density gradient centrifugation, none of the single fractios accounted for loaded infectivity, but some of the two-fold combinations were much more highy infections than expected from the contribution of single fraction. Therefore two components activate each other for infectivity.Protein analysis of slow- and fast- sedimenting components sugeested that a protein of about 50 KD MW is associated with infectivity. We interprete these and other results as indicating that a bipartit virus is associated with psorosis disease. The virus has ss RNA as its genetic material and a 50 Kd polypeptide as one of its structural protein(s).	
Categoria do assunto: 	--	
Registro original: 	Embrapa Mandioca e Fruticultura (CNPMF)	
Exemplares: 	CNPMF - (13324-1) - ADC - PL - -- - 634.3040601 - C748p - 1992000183 - DIS