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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
07/03/2012 |
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Data da última atualização: |
21/10/2015 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
SARAIVA, N. Z.; OLIVEIRA, C. S.; TETZNER, T. A. D.; LIMA, M. R. de; NICIURA, S. C. M.; GARCIA, J. M. |
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Afiliação: |
UNESP/JABOTICABAL; UNESP/JABOTICABAL; UNESP/JABOTICABAL; UNESP/JABOTICABAL; SIMONE CRISTINA MEO NICIURA, CPPSE; UNESP/JABOTICABAL. |
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Título: |
Effects of demecolcine on the meiotic cell-cycle and microtubular kinetics of activated bovine oocytes submitted to chemical enucleation. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
In: ANUAL CONFERENCE OF INTERNATIONAL EMBRYO TRANSFER SOCIETY, 24., Phoenix, Arizona. Phoenix: CSIRO, 2012 |
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Páginas: |
p. 119 |
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Idioma: |
Português |
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Conteúdo: |
Enucleation in traditional nuclear transfer (NT) is an invasive procedure for which alternative protocols have been sought. The present study was designed to explore the time course effects of demecolcine, a microtubule-depolymerizing agent, in bovine-activated oocytes submitted to induced chemical enucleation. For that purpose, after 26 h of in vitro maturation, the oocytes were parthenogenetically activated (5 mMionomycin for 5 min and 10 mgmL 1 cycloheximide for 4 h) and treated with demecolcine (0.05 mgmL 1 for 2 h) 2 h after activation. Three groups were established: control (untreated oocytes), activated (oocytes exposed to activation) and deme (oocytes activated and treated with demecolcine). Then the nuclear and microtubular dynamics of the oocytes were evaluated by immunofluorescence microscopy of tubulin and chromatin (Liu et al. 1998 Biol. Reprod. 59, 537?545). In each one of 3 replicates, 15 to 30 oocytes were evaluated per group. Oocytes were classified according to microtubule (MT) patterns as follows: present (evident MT), reduced (MT with reduced density), or absent. The results in percentage were submitted toANOVAand means were compared by Tukey test, with a significance level of 5%. Effects of activation were observed after 2 h, when higher rates of oocytes presenting second polar body (2nd PB) extrusion were observed in the groups activated and deme (49.3% in both groups) compared with control (1.7%). At the end of activation treatment (4 h), the activated group presented 81.8% of oocytes with 2nd PB extrusion, whereas it was observed only in 37.8% of oocytes in the deme group. Effects of demecolcine on the microtubules initiated after only 0.5 h of treatment, when an increase (Po0.05) of oocytes with reduced MT was observed in the deme group (26%; control ? 3%; activated ? 0%). After 6 h of culture in demecolcine-free medium, the deme group displayed ,50% of oocytes with reduced MT (control ? 0%; activated ? 39%) and absence of MT in 23% of oocytes, which was superior to other groups (control ? 0%; activated ? 2%). Therefore, we detected a reduction of MT density after exposition of activated oocytes to demecolcine. However, MT were not completely absent in most of the evaluated oocytes, as previously described for bovine oocytes submitted to chemically assisted enucleation (Saraiva et al. 2009 Cloning Stem Cells 11, 141?152; Meng et al. 2011 Cell. Reprogram., in press). Apparently, there was no immediate repolymerization of MT after culture in demecolcine-free medium and this could result in negative consequences for subsequent embryo development. Moreover, demecolcine impaired the second PB extrusion during the activation process, probably due to inhibition of spindle rotation caused by the MT-disrupting drug. Nonetheless, considering the higher cytoplasmatic volume obtained with chemical enucleation and the lesser extent of injuries suffered by recipient oocytes, further studies focusing on the potential of embryo development and the quality of embryos are advisable. MenosEnucleation in traditional nuclear transfer (NT) is an invasive procedure for which alternative protocols have been sought. The present study was designed to explore the time course effects of demecolcine, a microtubule-depolymerizing agent, in bovine-activated oocytes submitted to induced chemical enucleation. For that purpose, after 26 h of in vitro maturation, the oocytes were parthenogenetically activated (5 mMionomycin for 5 min and 10 mgmL 1 cycloheximide for 4 h) and treated with demecolcine (0.05 mgmL 1 for 2 h) 2 h after activation. Three groups were established: control (untreated oocytes), activated (oocytes exposed to activation) and deme (oocytes activated and treated with demecolcine). Then the nuclear and microtubular dynamics of the oocytes were evaluated by immunofluorescence microscopy of tubulin and chromatin (Liu et al. 1998 Biol. Reprod. 59, 537?545). In each one of 3 replicates, 15 to 30 oocytes were evaluated per group. Oocytes were classified according to microtubule (MT) patterns as follows: present (evident MT), reduced (MT with reduced density), or absent. The results in percentage were submitted toANOVAand means were compared by Tukey test, with a significance level of 5%. Effects of activation were observed after 2 h, when higher rates of oocytes presenting second polar body (2nd PB) extrusion were observed in the groups activated and deme (49.3% in both groups) compared with control (1.7%). At the end of activation treatment (4 h), the activated... Mostrar Tudo |
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Palavras-Chave: |
Demecolcine; Meiotic cell cycle. |
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Thesaurus Nal: |
oocytes. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 03762nam a2200217 a 4500
001 1917825
005 2015-10-21
008 2012 bl uuuu u00u1 u #d
100 1 $aSARAIVA, N. Z.
245 $aEffects of demecolcine on the meiotic cell-cycle and microtubular kinetics of activated bovine oocytes submitted to chemical enucleation.
260 $aIn: ANUAL CONFERENCE OF INTERNATIONAL EMBRYO TRANSFER SOCIETY, 24., Phoenix, Arizona. Phoenix: CSIRO$c2012
300 $ap. 119
520 $aEnucleation in traditional nuclear transfer (NT) is an invasive procedure for which alternative protocols have been sought. The present study was designed to explore the time course effects of demecolcine, a microtubule-depolymerizing agent, in bovine-activated oocytes submitted to induced chemical enucleation. For that purpose, after 26 h of in vitro maturation, the oocytes were parthenogenetically activated (5 mMionomycin for 5 min and 10 mgmL 1 cycloheximide for 4 h) and treated with demecolcine (0.05 mgmL 1 for 2 h) 2 h after activation. Three groups were established: control (untreated oocytes), activated (oocytes exposed to activation) and deme (oocytes activated and treated with demecolcine). Then the nuclear and microtubular dynamics of the oocytes were evaluated by immunofluorescence microscopy of tubulin and chromatin (Liu et al. 1998 Biol. Reprod. 59, 537?545). In each one of 3 replicates, 15 to 30 oocytes were evaluated per group. Oocytes were classified according to microtubule (MT) patterns as follows: present (evident MT), reduced (MT with reduced density), or absent. The results in percentage were submitted toANOVAand means were compared by Tukey test, with a significance level of 5%. Effects of activation were observed after 2 h, when higher rates of oocytes presenting second polar body (2nd PB) extrusion were observed in the groups activated and deme (49.3% in both groups) compared with control (1.7%). At the end of activation treatment (4 h), the activated group presented 81.8% of oocytes with 2nd PB extrusion, whereas it was observed only in 37.8% of oocytes in the deme group. Effects of demecolcine on the microtubules initiated after only 0.5 h of treatment, when an increase (Po0.05) of oocytes with reduced MT was observed in the deme group (26%; control ? 3%; activated ? 0%). After 6 h of culture in demecolcine-free medium, the deme group displayed ,50% of oocytes with reduced MT (control ? 0%; activated ? 39%) and absence of MT in 23% of oocytes, which was superior to other groups (control ? 0%; activated ? 2%). Therefore, we detected a reduction of MT density after exposition of activated oocytes to demecolcine. However, MT were not completely absent in most of the evaluated oocytes, as previously described for bovine oocytes submitted to chemically assisted enucleation (Saraiva et al. 2009 Cloning Stem Cells 11, 141?152; Meng et al. 2011 Cell. Reprogram., in press). Apparently, there was no immediate repolymerization of MT after culture in demecolcine-free medium and this could result in negative consequences for subsequent embryo development. Moreover, demecolcine impaired the second PB extrusion during the activation process, probably due to inhibition of spindle rotation caused by the MT-disrupting drug. Nonetheless, considering the higher cytoplasmatic volume obtained with chemical enucleation and the lesser extent of injuries suffered by recipient oocytes, further studies focusing on the potential of embryo development and the quality of embryos are advisable.
650 $aoocytes
653 $aDemecolcine
653 $aMeiotic cell cycle
700 1 $aOLIVEIRA, C. S.
700 1 $aTETZNER, T. A. D.
700 1 $aLIMA, M. R. de
700 1 $aNICIURA, S. C. M.
700 1 $aGARCIA, J. M.
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Embrapa Pecuária Sudeste (CPPSE) |
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| Registros recuperados : 71 | |
| 41. |  | OLIVEIRA, C. S.; SARAIVA, N. Z.; CRUZ, M. H. C.; MAZETI, B.; OLIVEIRA, L. Z.; LOPES, F. L.; GARCIA, J. M. HDAC inhibition decreases XIST expression on female IVP bovine blastocysts. Reproduction, v. 145, n. 1, p. 9-17, 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Gado de Leite. |
|    |
| 42. | | NICIURA, S. C. M.; FERREIRA, C. R.; PERECIN, F.; YAMAZAKI, W.; LEAL, C. L. M.; GARCIA, J. M.; MEIRELLES, F. V. Desenvolvimento embrionário, visualização de pronúcleos e transferência pronuclear após centrifugação de zigotos bovinos em meio com citocalasina. São Carlos, SP: Embrapa Pecuária Sudeste, 2007 34 p. (Embrapa Pecuária Sudeste, Boletim de pesquisa e desenvolvimento,11). ISSN 1981-2078| Tipo: Boletim de Pesquisa e Desenvolvimento |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
|  |
| 43. | | MEO, S. C.; FERREIRA, C. R.; PERECIN, F.; SARAIVA, N. Z.; TETZNER, T. A. D.; YAMAZAKI, W.; LEA, C. L. V.; MEIRELLES, F. V.; GARCIA, J. M. Karyoplast exchange between strontium- and 6-DMAP-parthenogenetically activated zygotes of cattle. Animal Reproduction Science, v. 116, n. 3-4, p. 381-385, dec. 2009.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 44. | | OLIVEIRA, C. S.; SARAIVA, N. Z.; LIMA, M. R. de; OLIVEIRA, L. Z.; SERAPIÃO, R. V.; BORGES, C. A. V.; GARCIA, J. M.; CAMARGO, L. S. de A. Kinetics data from bovine sex-specific embryo development from three different bulls. Data in Brief, v. 7, p. 1211-1216, June 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 4 |
| Biblioteca(s): Embrapa Amazônia Oriental; Embrapa Gado de Leite. |
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| 45. | | FERREIRA, C. R.; MEIRELLES, F. V.; YAMAZAKI, W.; CHIARATTI, M. R.; NICIURA, S. C. M.; PERECIN, F.; SMITH, L. C.; GARCIA, J. M. The kinetics of donor cell mtDNA in embryonic and somatic donor cell-derived bovine embryos. Cloning and Stem Cells. v. 9, n. 4, p. 618-629, dec. 2007.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 46. | | SARAIVA, N. Z.; OLIVEIRA, C. L.; DEL COLLADO, C. L.; LIMA, M. R. de; VANTINI, R.; MONTEIRO, F. M.; NICIURA, S. C. M.; GARCIA, J. M. Effects of trichostatina, a chromatin-modifying agent, on meiotic progression of bovine oocytes and embryonic development. In: CONGRESSO BRASILEIRO BRASILEIRO DE GENÉTICA, 59., 2013, Águas de Lindóia. Resumos... Águas de Lindóia: SBG, 2013. p. 54.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 47. | | SARAIVA, N. Z.; OLIVEIRA, C. S.; DEL COLLADO, M.; LIMA, M. R. DE; VANTINI, R.; MONTEIRO, F. M.; NICIURA, S. C. M.; GARCIA, J. M. Effects of Trichostatin A, A chromatin-modifying agent, on meiotic progression of bovine oocutes and embryonic development. In: CONGRESSO BRASILEIRO BRASILEIRO DE GENÉTICA, 59., 2013, Águas de Lindóia. Resumos... Águas de Lindóia: Sociedade Brasileira de Genética, 2013.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 48. | | COLLADO, M. del; SARAIVA, N. Z.; LOPES, F. L.; GASPAR, R. C.; PADILHA, L. C.; COSTA, R. R.; ROSSI, G. F.; VANTINI, R.; GARCIA, J. M. Influence of bovine serum albumin and fetal bovine serum supplementation during in vitro maturation on lipid and mitochondrial behaviour in oocytes and lipid accumulation in bovine embryos. Reproduction, Fertility and Development, v. 28, n. 11, p. 1721-1732, 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 49. | | FERREIRA, C. R.; BURGSTALLER, J. P.; PERECIN, F.; GARCIA, J. M.; CHIARATTI, M. R.; MÉO, S. C.; MULLER, M.; SMITH, L. C.; MEIRELLES, F. V.; STEINBORN, R. Pronounced segregation of donor mitochondria introduced by bovine Ooplasmic tranfer to the female germ-line. Biology of Reproduction, v. 82, n. 03, p. 563-571, mar. 2010.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 51. | | SARAIVA, N. Z.; OLIVEIRA, C. S.; COLLADO, M. del; LIMA, M. R. de; VANTINI, R.; MONTEIRO, F. M.; NICIURA, S. C. M.; GARCIA, J. M. Protocol optimization and evaluation of maturation promoting factor and mitogen-activated protein kina sea ctivities in bovine cytoplasts obtained by chemical enucleation techniques. Reproduction Fertility Devevelopment, v. 26, n. 1, p. 130, 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 52. | | SARAIVA, N. Z.; OLIVEIRA, C. S.; COLLADO, M. DEL; LIMA, M. R. DE; VANTINI, R.; MONTEIRO, F. M.; NICIURA, S. C. M.; GARCIA, J. M. Protocol optimization and evaluation of maturation promoting factor and mitogen-activated protein kinase activities in bovine cytoplasts obtained by chemical enucleation techniques. Reproduction Fertility and Development, v. 26, n. 1, p. 130, 2014. Suplemento. Proceedings da Annual Conference of the International Embryo Transfer Societty, 2014, Reno.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 53. | | MONTEIRO, F. M.; MERCADANTE, M. E.; BARROS, C. M.; SATRAPA, R. A.; SILVA, J. A.; OLIVEIRA, L. Z.; SARAIVA, N. Z.; OLIVEIRA, C. S.; GARCIA, J. M. Reproductive tract development and puberty in two lines of Nellore heifers selected for postweaning weight. Theriogenology, v. 80, n. 1, p. 10-17, 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 54. | | SARAIVA, N. Z.; OLIVEIRA, C. S.; TETZNER, T. A. D.; SOUZA, M. M.; LIMA, M. R.; NICIURA, S. C. M.; GARCIA, J. M. Métodos alternativos de enucleação oocitária utilizados na transferência nuclear em animais. Revista Brasileira de Reprodução Animal, v. 34, n. 4, p. 197-205, out./dez. 2010.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 3 |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 55. | | OLIVEIRA, C. S.; SARAIVA, N. Z.; LIMA, M. R.; SERAPIÃO, R. V.; OLIVEIRA, L. Z.; MARTINS, C. B.; VANTINI, R.; GARCIA, J. M.; CAMARGO, L. S. de A. Roles of cell death in sexual dimorphism during preimplantation development Animal Reproduction, v. 12, n. 3, p. 772, 2015. Edição dos proceedings do 29º Annual Meeting of the Brazilian Embryo Technology Society, 2015, Gramado.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental; Embrapa Gado de Leite. |
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| 56. | | FEUCHARD, V. L. da S.; MARQUES, S. C. de S.; SERAPIÃO, R. V.; SARAIVA, N. Z.; LIMA, M. R. de; GARCIA, J. M.; OLIVEIRA, C. S. Use of lipolysis supplement during IVM reduces lipid content of bovine oocytes but do not affect blastocyst cryosurvival. In: Annual Meeting of the Braziliam Embrio Technology Society, 33rd. Resumo em Anais. Ilha de Comandatuba, Bahia. SBTE, 2019. p 644.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 57. | | MONTEIRO, F. M.; MERCADANTE, M. E. Z.; BONILHA, S. F. M.; OLIVEIRA, L. Z.; SARAIVA, N. Z.; OLIVEIRA, C. S.; GARCIA, J. M. Ultrasonographic evaluation of reproductive tract measures and fat thickness traits in pre-pubertal nellore heifers. Boletim de Indústria Animal, v. 69, n. 2, p. 129-135, 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 4 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 58. | | TETZNER, T. A. D.; SARAIVA, N. Z.; PERECIN, F.; FERREIRA, C. R.; MÉO, S. C.; OLIVEIRA, C. S.; MELO, D. S.; MONTEIRO, F. M.; VANTINI, R.; GARCIA, J. M. Avaliação da influência da ovalbumina (OVA) como suplemento protéico durante a etapa de fecundação in vitro de oócitos bovinos. Acta Scientiae Veterinariae, v. 35, supl. 3, p. s1181, 2007; In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 21., 2007, Salvador. Anais... Salvador: UFRGS, 2007.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 59. | | TETZNER, T. A. D.; SARAIVA, N. Z.; PERECIN, F.; FERRERIA, C. R.; NICIURA, S. C. M.; OLIVEIRA, C. MELO, D. S.; MELO, D. S.; MONTEIRO, F. M.; VANTINI, R.; GARCIA, J. M. Avaliação da qualidade em embriões PIV com a substituição do soro fetal bovino e da albumina sérica bovina pela ovalbumina. Acta Scientiae Veterinariae, v. 36, (Supl. 2), 2008.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 60. | | SARAIVA, N. Z.; OLIVEIRA, C. S.; LEAL, C. L. V.; LIMA, M. R. de; CALLADO, M. del; VANTINI, R.; MONTEIRO, F. M.; NICIURA, S. C. M.; GARCIA, J. M. Chemically induced enucleation of activated bovine oocytes: chromatin and microtubule organization and production of viable cytoplasts. Zygote, v. 23, n. 6, p. 852-862, Dec. 2015.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| Registros recuperados : 71 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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