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![](/consulta/web/img/deny.png) | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo |
Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
10/05/2022 |
Data da última atualização: |
10/05/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SARAIVA, N. Z.; OLIVEIRA, C. S.; ALMEIDA, N. N. C.; FIGUEIRO, M. R.; QUINTAO, C. C. R.; GARCIA, J. M. |
Afiliação: |
NAIARA ZOCCAL SARAIVA, CNPGL; CLARA SLADE OLIVEIRA, CNPGL; NATHALIA N. C. ALMEIDA, Universidade Federal do Pará; MARIVALDO RODRIGUES FIGUEIRO, CPATU; CAROLINA CAPOBIANGO ROMANO QUINTAO, CNPGL; JOAQUIM M. GARCIA, Universidade Estadual Paulista. |
Título: |
Epigenetic modifiers during in vitro maturation as a strategy to increase oocyte competence in bovine. |
Ano de publicação: |
2022 |
Fonte/Imprenta: |
Theriogenology, v. 187, p. 95-101, 2022. |
DOI: |
https://doi.org/10.1016/j.theriogenology.2022.04.014 |
Idioma: |
Inglês |
Conteúdo: |
Oocyte in vitro maturation (IVM) is a key procedure for the in vitro production (IVP) of bovine embryos; however, the efficiency of this step is limited by the intrinsic developmental competence of oocytes. This study aimed to investigate possible epigenetic changes resulting from using of histone deacetylase (HDAC) inhibitors in the maturation of bovine oocytes and subsequent embryo development. Initially, we investigated the meiotic progression of bovine oocytes matured in vitro in the presence of trichostatin A (TSA). We then evaluated histone H3k9 acetylation levels in oocytes exposed to different TSA concentrations, and the relative expression of genes linked to oocyte competence. Finally, we studied preimplantation embryonic development by analyzing the cleavage, blastocysts, and hatching rates. Acetylation levels of H3k9 increased (p < 0.05) when oocytes were exposed to 50 nM or 100 nM TSA during IVM, but there were no significant changes in the relative expression of the evaluated genes p34cdc2, cyclin B1, MAPK, GDF9, G6PDH, and HSP70. We found that 5 nM TSA promoted the attenuation of meiotic progression and positively affected pre-implantation embryo development in bovine species, allowing a 10% increase in the blastocyst rate. We concluded that TSA treatment during IVM was efficient in promoting changes in H3k9 acetylation levels from 50 nM and promoted attenuated meiotic progression in bovine oocytes at all concentrations evaluated, with a positive impact on pre-implantation development when used at low concentrations. MenosOocyte in vitro maturation (IVM) is a key procedure for the in vitro production (IVP) of bovine embryos; however, the efficiency of this step is limited by the intrinsic developmental competence of oocytes. This study aimed to investigate possible epigenetic changes resulting from using of histone deacetylase (HDAC) inhibitors in the maturation of bovine oocytes and subsequent embryo development. Initially, we investigated the meiotic progression of bovine oocytes matured in vitro in the presence of trichostatin A (TSA). We then evaluated histone H3k9 acetylation levels in oocytes exposed to different TSA concentrations, and the relative expression of genes linked to oocyte competence. Finally, we studied preimplantation embryonic development by analyzing the cleavage, blastocysts, and hatching rates. Acetylation levels of H3k9 increased (p < 0.05) when oocytes were exposed to 50 nM or 100 nM TSA during IVM, but there were no significant changes in the relative expression of the evaluated genes p34cdc2, cyclin B1, MAPK, GDF9, G6PDH, and HSP70. We found that 5 nM TSA promoted the attenuation of meiotic progression and positively affected pre-implantation embryo development in bovine species, allowing a 10% increase in the blastocyst rate. We concluded that TSA treatment during IVM was efficient in promoting changes in H3k9 acetylation levels from 50 nM and promoted attenuated meiotic progression in bovine oocytes at all concentrations evaluated, with a positive impact on pre-... Mostrar Tudo |
Palavras-Chave: |
Cromatina; Epigenética; Oocito; Tricostatina A. |
Thesagro: |
Bovino; Embrião; Reprodução Animal. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 02377naa a2200277 a 4500 001 2142833 005 2022-05-10 008 2022 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1016/j.theriogenology.2022.04.014$2DOI 100 1 $aSARAIVA, N. Z. 245 $aEpigenetic modifiers during in vitro maturation as a strategy to increase oocyte competence in bovine.$h[electronic resource] 260 $c2022 520 $aOocyte in vitro maturation (IVM) is a key procedure for the in vitro production (IVP) of bovine embryos; however, the efficiency of this step is limited by the intrinsic developmental competence of oocytes. This study aimed to investigate possible epigenetic changes resulting from using of histone deacetylase (HDAC) inhibitors in the maturation of bovine oocytes and subsequent embryo development. Initially, we investigated the meiotic progression of bovine oocytes matured in vitro in the presence of trichostatin A (TSA). We then evaluated histone H3k9 acetylation levels in oocytes exposed to different TSA concentrations, and the relative expression of genes linked to oocyte competence. Finally, we studied preimplantation embryonic development by analyzing the cleavage, blastocysts, and hatching rates. Acetylation levels of H3k9 increased (p < 0.05) when oocytes were exposed to 50 nM or 100 nM TSA during IVM, but there were no significant changes in the relative expression of the evaluated genes p34cdc2, cyclin B1, MAPK, GDF9, G6PDH, and HSP70. We found that 5 nM TSA promoted the attenuation of meiotic progression and positively affected pre-implantation embryo development in bovine species, allowing a 10% increase in the blastocyst rate. We concluded that TSA treatment during IVM was efficient in promoting changes in H3k9 acetylation levels from 50 nM and promoted attenuated meiotic progression in bovine oocytes at all concentrations evaluated, with a positive impact on pre-implantation development when used at low concentrations. 650 $aBovino 650 $aEmbrião 650 $aReprodução Animal 653 $aCromatina 653 $aEpigenética 653 $aOocito 653 $aTricostatina A 700 1 $aOLIVEIRA, C. S. 700 1 $aALMEIDA, N. N. C. 700 1 $aFIGUEIRO, M. R. 700 1 $aQUINTAO, C. C. R. 700 1 $aGARCIA, J. M. 773 $tTheriogenology$gv. 187, p. 95-101, 2022.
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Registros recuperados : 71 | |
3. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | GARCIA, J. M.; LIMA, M. R. de; SARAIVA, N. Z.; OLIVEIRA, C. S. Evaluation of L-carnitine supplementation on the production and vitrification of bovine embryos produced in vitro. Animal Reproduction, v. 14, n. 3, p. 882, Jul./Sept. 2017. Proceedings of the 31st Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Cabo de Santo Agostinho, PE, Brazil, August 17th to 19th, 2017. Abstracts.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Amazônia Oriental. |
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5. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | ROSA, P.; RAGAGNIN, M.; GARCIA, J. M. J.; OLIVEIRA, C. S. Cytoplasmic granules in oocytes do not influence embryonic and early fetal development in bovine. In: ANNUAL MEETING OF THE BRAZILIAN EMBRYO TECHNOLOGY SOCIETY, 33., 2019, Ilha da Comandatuba, BA. Proceedings... Ilha da Comandatuba, BA: SBTE, 2019. p. 609.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Gado de Leite. |
| ![Visualizar detalhes do registro](/consulta/web/img/visualizar.png) ![Acesso ao objeto digital](/consulta/web/img/pdf.png) ![Imprime registro no formato completo](/consulta/web/img/print.png) |
7. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | LIMA, M. R. de; SARAIVA, N. Z.; OLIVEIRA, C. S.; GARCIA, J. M. The use of linoleic acid in in vitro culture of bovine embryos and its effects on production and survival to vitrification. Animal Reproduction, v. 14, n. 3, p. 894, Jul./Sept. 2017. Proceedings of the 31st Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Cabo de Santo Agostinho, PE, Brazil, August 17th to 19th, 2017. Abstracts.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Amazônia Oriental. |
| ![Visualizar detalhes do registro](/consulta/web/img/visualizar.png) ![Acesso ao objeto digital](/consulta/web/img/pdf.png) ![Imprime registro no formato completo](/consulta/web/img/print.png) |
9. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | OLIVEIRA, C. S.; SARAIVA, N. Z.; OLIVEIRA, L. Z.; SERAPIÃO, R. V.; LIMA, M. R. DE; GARCIA, J. M. Global H3K9AC and H3K27ME3 expression in blastomeres from 8-to 16-cell stage bovine embryos. Reproduction, Fertility and Development , v. 26, n. 1, p. 148-149, 2014. Suplemento. Proceedings da Annual Conference of the International Embryo Transfer Societty, 2014, Reno.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Gado de Leite. |
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10. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | SARAIVA, N. Z.; FIGUEIRO, M. R.; MARQUES, J. R. F.; LIMA, M. R. de; GARCIA, J. M. Effect of in vitro co-culture of buffalo embryos with bovine cumulus cells on the potential for early embryonic development. Animal Reproduction, v. 14, n. 3, p. 858, Jul./Sept. 2017. Proceedings of the 31st Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Cabo de Santo Agostinho, PE, Brazil, August 17th to 19th, 2017. Abstracts.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Amazônia Oriental. |
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13. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | SARAIVA, N. Z.; PERECIN, F.; MÉO, S. C.; FERREIRA, C. R.; TETZNER, T. A. D.; VANTINI, R.; GARCIA, J. M. Efeitos da demecolcina sobre a cinética da maturação nuclear e a migração dos grânulos corticais em oócitos bovinos. Acta Scientiae Veterinariae, v. 35, supl. 3, p. s1276, 2007; In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 21., 2007, Salvador. Anais... Salvador: UFRGS, 2007. p. 1276.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Pecuária Sudeste. |
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17. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | SARAIVA, N. Z.; PERECIN, F.; MÉO, S. C.; FERREIRA, C. R.; TETZNER, T. A. D.; GARCIA, J. M. Demecolcine effects on microtubule kinetics and chemically assisted enucleation of bovine oocytes. Cloning and Stem Cells, v. 11, n. 1, p. 141-152, mar. 2009.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Pecuária Sudeste. |
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18. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | OLIVEIRA, C. S.; JEDRUSIK, A.; SARAIVA, N. Z.; CAMARGO, L. S. de A.; GARCIA, J. M.; ZERNICKA-GOETZ, M. PAR6C polarity protein expression pattern in male and female bovine embryos. Animal Reproduction, v. 11, n. 3, p. 455, 2014. Edição dos proceedings do 28º Annual Meeting of the Brazilian Embryo Technology Society, 2014, Natal.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Amazônia Oriental; Embrapa Gado de Leite. |
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19. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | MÉO, S. C.; YAMAZAKI, W.; FERREIRA, C. R.; PERECIN, F.; SARAIVA, N. Z.; LEAL, C. L. V.; GARCIA, J. M. Parthenogenetic activation of bovine oocytes using single and combined strontium, ionomycin and 6-dimethylaminopurine treatments. Zygote, v. 15, p. 295-306, nov. 2007.Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
Biblioteca(s): Embrapa Pecuária Sudeste. |
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20. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | ROSA, P. M. S.; MONTEIRO, C. A. S.; LEAL, G. R.; CAMARGO, A. J. R.; SERAPIÃO, R. V.; LIMA, M. R.; GARCIA, J. M.; OLIVEIRA, C. S. Association of two epigenetic modulators on the initial development of female bovine embryos. Animal Reproduction, v. 15, n. 3, p. 484, 2018. Edição dos proceedings do 32º Annual Meeting of the Brazilian Embryo Technology Society, Florianópolis, 2018.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Gado de Leite. |
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Registros recuperados : 71 | |
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Nenhum registro encontrado para a expressão de busca informada. |
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