Data/hora: 16/06/2024 05:13:00

Biblioteca(s): | Embrapa Caprinos e Ovinos.
Data corrente: | 23/08/2023
Data da última atualização: | 24/08/2023
Autoria: | LEÃO, U. S.; BUENO, L. G.; NEGREIROS, A. B.; SILVA, G. R.; MAGGIONI, R.; BRITTO. F. B.; SARMENTO, J. L. R.; GALVANI, D. B.; DINIZ, F. M.
Afiliação: | UESLEI S. LEÃO, Northeast Biotechnology Network (RENORBIO); UNIVERSIDADE FEDERAL DO PIAUÍ; LUICE GOMES BUENO GALVANI, CNPC; ALINE B. NEGREIROS, Northeast Biotechnology Network (RENORBIO); UNIVERSIDADE FEDERAL DO PIAUÍ; GEICE R. SILVA; RODRIGO MAGGIONI, INSTITUTE OF MARINE SCIENCES (LABOMAR), UNIVERSIDADE FEDERAL DO CEARÁ; FABIO B. BRITTO, UNIVERSIDADE FEDERAL DO PIAUÍ; JOSE L. R. SARMENTO, UNIVERSIDADE FEDERAL DO PIAUÍ; DIEGO BARCELOS GALVANI, CNPC; FABIO MENDONCA DINIZ, CNPC.
Título: | Unveiling the demographic background and genetic diversity of Urochloa mosambicensis (Poaceae) through genome-wide identification of simple sequence repeats and molecular marker development.
Ano de publicação: | 2023
Fonte/Imprenta: | Conservation Genetics Resources, v. 15, p. 1-9, Aug. 2023.
DOI: | https://doi.org/10.1007/s12686-023-01312-8
Idioma: | Inglês
Conteúdo: | Abstract: The first set of simple sequence repeat (SSR) markers for Urochloa mosambicensis (Hack.) Dandy is described in this study. SSRs were isolated from a genomic library with 3491 contigs obtained by high-throughput sequencing. Primers were designed for twenty loci, and all primer pairs were successfully amplified in 39 genotypes, yielding an average of 4.95 alleles per locus. The mean polymorphic information content (PIC) was 0.559, and the mean discriminating power (DP) was 0.520 for all loci considering also all U. mosambicensis genotypes. The non?Bayesian clustering analysis has confirmed clustering consistency of the individuals and provides enough support for the existence of the pattern that also emerged from PCoA and UPGMA. These markers demonstrated potential transferability to U. advena, U. oligotricha, U. brachyura and U. xantholeuca. Our findings showed that the set of molecular markers described here have strong potential for fingerprinting and characterizing genetic diversity in intra-specific plant collections, whose breeding programs could be accelerated by the effective use of these molecular tools, not only in U. mosambicensis, but also within the genus Urochloa.
Palavras-Chave: | Capim-corrente; Genetic diversity; High-throughput sequencing; Microsatellites; Next-generation sequencing; SSR; Transferability.
Thesagro: | Capim Urochloa; Genética Molecular; Gramínea Forrageira; Marcador Genético; Marcador Molecular; Urochloa Mosambicensis.
Thesaurus Nal: | Forage grasses; Genetic markers; Molecular genetics; Plant genetics; Sequence analysis.
Categoria do assunto: | G Melhoramento Genético
Registro original: | Embrapa Caprinos e Ovinos (CNPC)
Exemplares: | CNPC - (40608-1) - UPC - AP - DD -  -  -  - DIS