Data/hora: 23/09/2024 13:59:59

Biblioteca(s): 	Embrapa Mandioca e Fruticultura.	
Data corrente: 	14/08/1995	
Data da última atualização: 	17/04/2023	
Autoria: 	SHALITIN, D.; MAWASSI, M.; GAFNY, R.; LEITNER, O.; CABILLY, S.; ESHHAR, Z.; BAR-JOSEPH, M.	
Afiliação: 	Department of Biological Sciences, Department of Membrane and Biophysics and Department of Chemical Immunology, The Weizman Institute of Science, Rehovot, Israel.	
Título: 	Serological characterisation of citrus tristeza virus isolates from Israel.	
Ano de publicação: 	1994	
Fonte/Imprenta: 	Annals of Applied Biology, v.125, n.1, p.105-113, 1994	
ISSN: 	0003-4746	
Idioma: 	Inglês	
Conteúdo: 	Seven monoclonal antibodies (MAbs) showing homologous reactions with the VT strain of tristeza virus (CTV) were tested against 21 CTV strains or isolates, representing the range of biological diversity and the geographical distribution of CTV in Israel. All the CTV strains gave positive reaction in ELISA with polyclonal antibodies and with one MAb (#25/2). Two MAbs; #13/21 and #3/7 reacted with 19 and 12 out of the 21 CTV strains, respectively. Seventeen CTV strains, including all those previously assigned by sequencing their coat protein gene (CPG) to the CPG-VT group (Mawassi, Gafny & Bar-Joseph, 1993) reacted with five or more MAbs. Four CTV strains of minor epidemiological importance, including two members of the CPG-MT group, did not react with five or more of the MAbs. These results indicated the existence of two serogroups of Israeli CTV strains that can be differentiated by MAbs and which closely correlate with and extend the previous CPG grouping. The extensive biological variation within each CPG group confirms recent analyses suggesting that the CTV pathogenic traits are not necessarily associated with a sequence or antigenic variation of the CTV-CPG.	
Palavras-Chave: 	Aphid transmissibility; Coat protein gene; Pathogenic isolates; Tristeza-epidemiology.	
Thesaurus Nal: 	monoclonal antibodies.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Mandioca e Fruticultura (CNPMF)	
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